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Ammonium, nitrate, and nitrite in the oligotrophic ocean

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Material Information

Title:
Ammonium, nitrate, and nitrite in the oligotrophic ocean detection methods and usefulness as tracers
Physical Description:
Book
Language:
English
Creator:
Masserini, Robert T
Publisher:
University of South Florida
Place of Publication:
Tampa, Fla.
Publication Date:

Subjects

Subjects / Keywords:
Chemical
Oceanography
Submersible
Analyzer
Sensor
Auv
Ammonium
Nitrate
Nitrite
Dissertations, Academic -- Marine Science -- Doctoral -- USF   ( lcsh )
Genre:
government publication (state, provincial, terriorial, dependent)   ( marcgt )
bibliography   ( marcgt )
theses   ( marcgt )
non-fiction   ( marcgt )

Notes

Summary:
ABSTRACT: The overall focus of this research was to achieve the first detailed understanding of temporal and geographical distributions of inorganic-nitrogen-nutrients within an oligotrophic euphotic zone. In addition to low supply of nutrients, the uptake of nutrients by phytoplankton within the euphotic zone draws the nutrient concentrations down, resulting in very low concentrations of these nutrients and results in these regions being classified as oligotrophic. The site selected for the research was the West Florida Shelf (WFS). There were two main challenges. One was that the detection limits of the standard chemistries used to determine inorganic nitrogen nutrients are not low enough to permit the evaluation of the concentration of these nutrients within an oligotrophic euphotic zone.The other challenge was the adaptation and design of highly sensitive, robust, and simple instrumentation to resolve and evaluate horizontal nutrient distributions within the euphotic zone for both ship-based and Autonomous Underwater Vehicle (AUV) based platforms in near real time. With these obstacles in mind three major goals were set. First was the development of a simple and robust chemistry that could detect nitrite and nitrate with a suitably low detection limit (approximately 10 nanomolar) and could also be coupled with a highly sensitive chemistry previously developed for ammonium with the same characteristics, and incorporate these chemistries into a single laboratory analyzer designed to monitor the surface distribution of these nutrients in the water sampled with a ships flow-through system.
Thesis:
Thesis (Ph.D.)--University of South Florida, 2005.
Bibliography:
Includes bibliographical references.
System Details:
System requirements: World Wide Web browser and PDF reader.
System Details:
Mode of access: World Wide Web.
Statement of Responsibility:
by Robert T. Masserini.
General Note:
Title from PDF of title page.
General Note:
Document formatted into pages; contains 211 pages.
General Note:
Includes vita.

Record Information

Source Institution:
University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 001681034
oclc - 62705742
usfldc doi - E14-SFE0001093
usfldc handle - e14.1093
System ID:
SFS0025414:00001


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Full Text

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61B $rft=67B7f#rn#)rfnf#f;r*n 760E76. f5ff?r5r-$;fr$67B1rrfnfrrffnf ffr f+rfrrf @>870AE87/. ,frff #67B6rfnfrffnnfnrfnnf fnf2fnrfr#rn &>607E6// ,f+)r#&f(*;(trrr?;rnr(n f5$)f%r -f f?r$tnrnf%ffnn 3ftf&" nrrnf-rn&f$r-fn5rr 3ftf \#nrnf$rrrfnnr -f 67BBnrtfnr#nrnf$r%rfr"r9 -f(r f-fn 6776fr*fr rf2fnr f+rfrrf @9BA@@EBA.0 -f)n5+-rf,f+nr"?r-) $r"5fr&f(fRf 677A +fr*frrffn%nnr2rn&fr rnffffnfrn+rfrrf;rnnr (.6A8AE6AA0 -rrr"f(5f5$6770rnnfnrrnr rffn"f ,06/8AE6/AB -f=frr/006tnrnnnffnrnr nrfnnfnrf2nrrrrnfffrnfn rr$rfrrf .=AA1EA7@
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686 bt47fbt8 fnfr2fnfnfr*rnrnrnf3fn5r*nnnrF$rr &rGrfrnnrrrfrrrnf5r*nnnrF$rr&r /GrrfnrEnrrrrnrfrF$rr&r@Grt9EHr%r %rf;f-2K#,9$nnnrnfrnrff%rf &r@G&nrErrrnnnffnt9EH n2fr nfnrffEnEnf%rnrfnnrnrfrn nrH#fE6E68I=E10fnff%ffrnfnnrnrtr*n rnrt9EHrfrrnfnfnn2frnfrfrrnr ffff%r#rrrrrrfr2r%rf5( Frr&r.Grnr#fnrnrrnfr&r. rrnr%rf9nrfrfnrrrnnrH#fE9E68I=E10Fr%r6GFn Gr5nr"rnrnnFnGfrrnnr9nfnr 2nfnrFrr&rGr"fnKfrrrnE60,ED60,f rrrrnrrrr$r=rf&rrnrr%r frrr5(fr*nnrt9EHrfrrnfnfn =*rrffZnEnf%rnrfrfnf!rf rffffrfnrnfnnfnnrfnff!nfrnrfr2nnr H#fE9E68I=E10%ffrf5n*Ftfnf9nrn$#?E8BGffr rnr8BfrFtfnf9nrn6B.A.7#E06Gfnrnf68rnrnr frFtfnf9nrn6B@187#Gnfnrnrn2rrnrH#f

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68/ bt47fbt8 & r-2frfnrt9EHnfrnf3r

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68@ bt47fbt8 & r/-2fr;rrfrnfrt9EHn2fr

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68. bt47fbt8 & r@-2fr2rrrfnrt9EHn2frfrr ?nnrt9EHr%r%rf;f-K#,9$nnrnfr

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681 bt47fbt8 & r.-2fr2rrrrrrt9EHrfrrnf nfnn2frfrrrnr?rfnV=%rnr9fIWrrnr 22rrnr

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688 bt47fbt8 & r-2fr2rrrnrnrH#fE9E68I=E10fr rrnrr%fr2f%rnrrrnrnrfnnr nrnr

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68A bt47fbt8 fnrrrnrfrrrfn%rrnrfffnnr rnrfrnrnrnrfn8.FffG2nnrrf5n*r n%rrnrffffrrnrfrnnrfr rnrrnrnfr0fn8BF#)0Gnrnfnrnnrf rnrnfr6fn@@F#)6Gfnrffrnrn fr/fn81F#)/Grrfnrfnfrnfnnfnrnr nrfnfrnrn61f1/F+tf90rrn%rG2nf600 ffffnD1,rrnf1(rnf6. FD1,Gf1/F90Grrrnf+"$F+"$Gff&+"E@8 nrfnrnnrnr%ff?r5$?EnErf%rnrF&160@G%rnr rrnfnf%rrrfn2rnrfnfr r$?EnErf%rnrnfrfnrffnr>nn rnrnrffrfnnnrfrnrffnfnr2nnrfnf #rnn2fr 9rffrfrffnrnrfnfffnfrf rnnrfnf#rnn2frrnrrnnrfn2nnrn3fn n2frfffrrrrnrnrrrf%rrrnrnf5rfnf r%rnrnrnfrrnfnErrfnrnr tnrnfnf#rnn2frnfrrnnr#nfnr nffnrnnrnrfnrrnr*rnfr9fr% %rfnf#rnfrrnfrn2nr%rnrr%rnr n2frFrf%ffnfrnfnf%rrrrnrGrn2frrnr*rnfr

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68B bt47fbt8 nnrrffnnffnf#rn&nrErrrn nffnrnrn2frfrrnfrrnnrnr2rnrn nfnfrfnrrr ",bf @ rfrt(brtr*frf%nrt rtnrnfnf#rn$n2frfrnfnrrnrr5nr fnfrnnrr5nFrr&rGrrnnrfr fnr2nfnr @/ !ft!tb rfrrr2rnrtnrnfnf#rnn2frnfnr Frr&rAG=fnnnfnnfrEnfn%fnrrnrfErf rfnnnnfnfrEnrrf%ffrrfffnf r2rfr%frrfrrfrrnr nffnr2nfrnn @@ rb n%fnnr$rnnnFrr&rAGrf2nr$rn r&rBrnnrnfrrnnr2rrnrfnf ffrnrrrrnnrn2rrnnrrfnfrn fnfrfnrrrnfrfrnrfrrrfnf $r2rrnrnrfrnrrrrnrrnfr $nff&frrnr$nffrnnrfnff nfnfrf2rfrnnrfrnrrfnrr

PAGE 184

687 bt47fbt8 & rrtnrnfnf#rn$n2frnrr5nr frrfnfrnfnrrnrnn

PAGE 185

6A0 bt47fbt8 & rArtnrnfnf#rn$n2frfr2$rrnr*n rnf

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6A6 bt47fbt8 & rBrtnrnfnf#rn$n2fr$rn2$rrnr*n rnf

PAGE 187

6A/ bt47fbt8 nfnfrf2rfrnnrfrnrrfnrr &rnnr2rr%rfrfrf!r%ffr2ff nrr2rnr%rnn3rnrfr2r nr2nnr+"$nrrrnrnnr2rfrf!r rnrrfnffnnrff#frnrffr-r nrrrnnFrr&rAGfn%fnrnrrrnfnf$rrf 2rFrr&rG2rrnrfnfffnr2nnrfrfr rnrrrrfnffrnrfnr2nnrfnfrnfnnrfrf 2nnrnfrr$fr#nrnnF&rBG2rrnrrfr nfrrnf&fnf$rfrrnrrfrf rrnrnrnrffnnrrrrnf nn r$frfnrnnF&rBG2rrnrfnfffnf rffrrrrnn/00rrfnfrrnr f'nrrt/t@ft).rrnnfrrnfnnrrnr nrnnffrnnrffnffnnrnr2rnrrf5 ffrnrrrrrnrnrrnrrnrnrr2rr nrfr2fnfrfnnrnrfrrnrf%rrn2rr nrnr2rnrfr2fnrnnrffnfffnrnrnfn nrrf5rfrnrrrrrnrnr+"$nr2nf nrnffnrnffrrnrrnf5nrnrnff

PAGE 188

6A@ bt47fbt8 & rrtnrnfnf#rnrrn$frfnf2$rrr*n rnf

PAGE 189

6A. bt47fbt8 frrf5nrrnrfnrnrnnrfn nrnrnrnrrnrrrfn2rrnnFrr &rBGnrrnrf'nrrrnrnrfnrnrrnnr ffnfnrrnfnrrfnr/r#rn= rnn&rBrnrnnrrrrrn2rrnrnfnr rnfnnrnffrrnrrrnrnrnfnrfnnfnr fnfrrnnrnffrnfnrffnffrrnrnnnrnnr frnnrnfffnrnnrnrrnnfnrnrf5rf rrnrrrnrnrrfrffnr2rr rfnr5rnffnfnrfrrnfn'nnnrnfnrr f%rfrnrnfnrrf5rn%rnrf%rfrnrnnrrf5rnn rnrrrnrfrn%frrnrrrnrnr f2fnnrrrrnfnnr rnrrnrnrnfnrrnfnffrnnrnnr rnfnfnffrnnr$nff#fnrrrr rnrnnrfrnrnfnrrnfnrnrnfnrfr rrrnf$nfffnf$rrnffnrnrnrn rr r+"$#"nrnn&rBrnrnrrf fnnn2nr+"$nfrnr&nr fnf$rrrrnf$nffnfnnr)r%rf

PAGE 190

6A1 bt47fbt8 &nfnr2nnnr+"$nrnrfnrf+"$9K r2 r;fnnrf;nrff*rnn&rBfrrnrn nrrfnff*%frnrrffrnrnrrfEnrf5 fnrnrnrnfnnr&fnf$rrrr %frfrrnrrnnrrrrnfnnrrfrrr nrr%frFnr2rnnr"rrffn0rrrfrrnrrf rfn%r%frffrfnnrnnr=!fnG @. rftf%t bfrt -rfn%fnrnr$nff#fnnnFrr&rAGrf 2Frr&rG2rrnrrrnrfrnfnnrnff nfnfrnrnnrffnfnrfrrrrrrnrnrnfnr rfrnfnnrnfffrr3r;t$-rnrfE nrnErf2fnrfrnfnf%rnf5nff2nfnrf nffnrnfnrrnrf?9?rnrfrfrnfnff rrrfrr3r2fnrnrf%rfr2nfnr fnffFnrfnr9Gffrrnrnr& rrnfnf$rrnrnnrfnfnrfr9rnrfnffnfn nrr2nrfrnrnnrrfnffE rf%frrnrnrnf9?;t$-rnrrfnff rnfn2nrr3rf2r%rnrnf9fr!rn

PAGE 191

6A8 bt47fbt8 & rrtnrnfnf#rn$nff#fn22$rrnr*n rnf

PAGE 192

6AA bt47fbt8 rr9rnrnrrf5rrr*r*frf5rr rrnr%rnrnfff2rnnrrfrrrnfnn%r rfrrnrrnrrnfnnrrrnn rnfffnr%frfnffrnfnfnrffrrf!r fnrfnffrrnrrnrrnnrrnfn%fr ffn%fnrnr"rnnF&rAG @ #f rfrnfnffrfrrf!rrrrfnr;f nnfn%fnrFrr&rAG2rnr;f2&r nrfrrrnrfnr2rnfnf5nrrfn2rnrn nrnfffnrfr5Frr&r/G2rr nn!rnfrnrnfffnfffnfnnrr nfn2frnrfnnrnrr2frrrfrfnnfffr fnrffnfnffrfnnfnnrfrnrffn fnr $!#$3G!3%!$ nfnrnff nrfrfrnffrfr2rnnr!rnFrr &r/G2frnrfnfnrrr2nnrnfffnf ffnfnrnnr&r*frfrfn2rnrr nnrnfffnfffrfnfnf5rfr%ffnr r!rn2rrf!rnrr!rrrrrrr nffr2rrrf2rrnrE2Frr&r/Gn

PAGE 193

6AB bt47fbt8 & rrtnrnfnf#rn;f2$rrnr*nrnf & r/rtnrnfnf#rn;f$nff#fn&5 2$rrnr*nrnf

PAGE 194

6A7 bt47fbt8 fnrnfffn5rnfffnf2rrrrf rrrrr2nrrnrnfn$frrnrf nffrnrrrrnr%rff?f9nf fr2nrrrrf2rnrE2Frr&r/G fnnfnrnfffn5nrrnrnff fnnfnfrnrrrrrrnfffnf ffnfnnrffn%rnrrrrrnff 2nrrfrnnr2fr @ n n%fnnr"rnnFrr&rAGfrfrnrrf5 f5rrnr$frnnrrrnrfrfnrfnf rnfrnrtnrnfnf#rnn2fr9nrnrrfnf ffnrrfnfnf&r*frf$nfffnf$rr rrrnrrnfnr!rnrrn&fnf$r rfrnfnfnfrffnr9rnrfrnnf5rfrnnr r!rnrnrrfrnrnfffnrnnr rf2rtFrr&r/Gnr22rnrnnr nrnfffn5fnrfn%fnnr"rnnFrr&r AG-rnrrnnfn%fnrf2rFrr&r@G2rrnr !rnfnrnf5rr9rrrnrfff ffnrf5f5r2rnfnrnfnrnrnrr

PAGE 195

6B0 bt47fbt8 & r@rtnrnfnf#rnrnrf5r2$rrnr*n rnf f5rfrrn9rrnrffrf5f5rffnfr5rnff ffnrffnfrfrnrrf5r @A tf n%fnnr$fnnFrr&rAGfrnrnEfnfnr fffnrfrf2&r.fr*frf$nfffnf $rff%ffrfnrrfnf$rrrfr rnrnrrfrf2nrfrf5ffrfrfnr rrrf5f5rfrfnfnffrfnnfrfrrnn&nr ffrnrrf5rfrfrCfrrf5nrrfrr ffrfrrf5nrrf5nrfrff5rf5nrrfrr ffrrfrrffnrnrfrrrn2rrnrrf ff5rnffnrnrfrr%frrnrnrrf rf5f5r&nrnnrfnfnrfrn2f5rfrrfr

PAGE 196

6B6 bt47fbt8 f5rf5rrrf5rffrrf5f5r9nfrnfnnrr frrn%rnrnfnnrfrrfrn'rnrf5 f5rfrf'nfrnrffr%r5rfnrr*nr rf5f5rffnnnrrrnRnr5frrn f5rnrnrf5rfrrnfrn5nrfr 2fffrEnr*fnrfrfnrrn3f5n 5nrffnrrrnfrnr2f &r.&ff'nrrf5f5r%rrnnrrrnrnr ffnrrnfnffrnr"rnnnrrrFrr&r AG @B t n%fnnrrrnnFrr&rAGfrrfrr fnf$nfffnf$rrnrfrf&r rfrrnrnrrfrf2rrr2fr f%ffrfnfrnrnr$nfffnf$rrrrnrrf5 rnnfffnrrnfn%frrnrrnr$nff#fn FnfnrnnrrnrfrnrrGfrnnrnr n2f*rrrfn!frrfrrr!fnfrnrrrnf r2nnr / %frrrnrrf5rnfrnfnfrnnr f*rf%rrr2nrnrrnffffnfnnfnnr rfnf!5rn2rnrnrnrrn%frfrffrn

PAGE 197

6B/ bt47fbt8 & r.rtnrnfnf#rn$f2$rrnr*nrnf

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6B@ bt47fbt8 & rrtnrnfnf#rnrr22$rrnr*nrnf

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6B. bt47fbt8 nrrnrnnnrrnrfrfrnfn rnfn9n2rnfnfnffrr%rnrrrff f5rrrf5rffrrf5f5r9nfrnfnnrr frrn%rnrnfnnrfrrfrn'rnrf5f5r rn5nr!frfnfffrrnr*rnrE 2nfnfrfFrr&rGrrfrn2nnE22 r*rfrnrrrnrrrnrfnr2frfnfn2rrnr rfnfnrrrrnrnrnrfnffrf5f 2rnrr*rnr%rnrrrnrrf5rrfrnnfnr nrrnrnrrr2r=*nnnr*nnr rrfnnf!fnnrnrnnrnrfnffr fr*nrfnrrffnfnfnfrf'nrf5f5r rrnrfrf5nr=*nnnrrrnrnffnr fnnrrnrrr!fnnrr"nnn nfnrnr>n25r-rnrrfrnnfn%fnrnrf 2Frr&rAG2fnrrfnnrrnnrfrnrf*rr rnnrnrfn%r22 @ bf n%fnnrrfnnFrr&rAGfrrffnf $nfffnf$rrnrfrf&rBrfr rnrnrrfrf2rrf2frf%ffr

PAGE 200

6B1 bt47fbt8 & rrtnrnfnf#rnrr=*rrnrE 2$rrnr*nrnf

PAGE 201

6B8 bt47fbt8 & rArtnrnfnf#rnrfrrr+f2$rrnr*n rnf

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6BA bt47fbt8 & rBrtnrnfnf#rnrf2$rrnr*nrnf

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6BB bt47fbt8 fnfrnrnr$nfffnf$rrrrffrffnrrf nffnfnnrfnfrnfnnrnfffrnrrnr $nff#fnnrrnfnnrnffffnrnrrf rrfnfffnrfnfrf5rnnrnffrrf frfr*rrn2frnr2rfffnfnrrfnr rfrfnnrrffn3rff%rfnff r%fnrnffr%fnnrrffrnr2rrnr nrrf2r#rnnnrrnrnr2r nrfn%rrf2 @ fn n%fnnrf5nnFrr&rAGr22fE rfnrnrnfnf&fnf$rrf &rrfrrnrnrrfrf2rr 2frf%ffr&fnf$rrrnfnf nrnfrrfnrfnrnrrf5f5rnf%rrrrnrr rnnrfrrnrrfrf%rnr$rnrnr nFfnnrfnrGfrnfnnrfnfrrfr frnrnfrnr2rrnfnfrfrrn rrnnrfrnnnnrrnnrfrf2Frr&r /0G2rrnrfrnrfnrnrnfnfrr rr%frfrrrnrnrffn

PAGE 204

6B7 bt47fbt8 & rrtnrnfnf#rnf52$rrnr*nrnf

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670 bt47fbt8 & r/0rtnrnfnf#rnf5rfr#rnfnfr 2$rrnr*nrnf

PAGE 206

676 bt47fbt8 nrrrrnfnnrrrrn%fnnrrfrnnrf 2F$rr&r/6G2rrnrfrnrffrrnrr%frff rrrnrnrffnnrr rrnfnnrrrrrrnnnrrnrnr 2rnrfn%rrf2F$rr&r/6G @ bt n%fnnrrrnnFrr&rAGr$f2nfnf frrfrfnrfnnnfnrrfnnn nrfrrnrrnrnrnfnfnnrnrrfnrfr nrrfnfnrnfnnrnrrfnrfnfnnnrfnE frnfnr2rfrfnfnrnrfnffr frffrnfnnr$frr2fnrrrnnEfnnr nrrrnrfnrrf5f5r @/ r n%fnnrnnnFrr&rAGnrfnrf#frr nf5r2rnfnfnnrrfrrnrrnr$rn22 $fr#nrnnnrrnfnfnnrfnfrnfn2rnrnnr nrffrnnnrffnfnrffnrrf5f5rnf frf%rrrfrfffrrrnfrrrnnr

PAGE 207

67/ bt47fbt8 & r/6rtnrnfnf#rnf5rfr#rnfn+f 2$rrnr*nrnf

PAGE 208

67@ bt47fbt8 @@ )&r n%fnnrnnnFrr&rAGff2rrnrfn %rfn2Frr&r//Grfr2ffnrfnr frrnrfrffnr2nnrfnr@. %f @. %fr n%fnnr#rfnnFrr&rAGrfr2-2 rnfnffrfnfrfrfrffrrnnrnnn fn%fnrnrrr2FG ".fbff rrrfnrnrn2frfrfrnrnnrnfrr nr$rnr2rrfrnrrfrnrnfnfrrrfnrnr tnrnfnf#rnn2frffnfrrrfnrnfnnrfnr f2fnfrnrfrrfrf%rf f4 r*nr#fn fnfrf5f5rfnffrfnrfffnf f%rfn r*nrrnrnrnfnrrfnrffnr*nrfnFnf rnrG2nf ntn r*nrrr ntn rnfnrffnrrnfn frf2rffnffnnrfnrrnfrf nnrnrrfrrffnr ntn rrfffnr rfrfrrnrfnrfnrnrnfnnr rrnfnf$rfnrr2n9$22rnrfnr fnr&fnf$rfnrr2n&;-2

PAGE 209

67. bt47fbt8 & r//rtnrnfnf#rnn%rfn2$rrnr*n rnf

PAGE 210

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PAGE 211

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Masserini, Robert T.
0 245
Ammonium, nitrate, and nitrite in the oligotrophic ocean
h [electronic resource] :
b detection methods and usefulness as tracers /
by Robert T. Masserini.
260
[Tampa, Fla.] :
University of South Florida,
2005.
502
Thesis (Ph.D.)--University of South Florida, 2005.
504
Includes bibliographical references.
516
Text (Electronic thesis) in PDF format.
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System requirements: World Wide Web browser and PDF reader.
Mode of access: World Wide Web.
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Title from PDF of title page.
Document formatted into pages; contains 211 pages.
Includes vita.
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ABSTRACT: The overall focus of this research was to achieve the first detailed understanding of temporal and geographical distributions of inorganic-nitrogen-nutrients within an oligotrophic euphotic zone. In addition to low supply of nutrients, the uptake of nutrients by phytoplankton within the euphotic zone draws the nutrient concentrations down, resulting in very low concentrations of these nutrients and results in these regions being classified as oligotrophic. The site selected for the research was the West Florida Shelf (WFS). There were two main challenges. One was that the detection limits of the standard chemistries used to determine inorganic nitrogen nutrients are not low enough to permit the evaluation of the concentration of these nutrients within an oligotrophic euphotic zone.The other challenge was the adaptation and design of highly sensitive, robust, and simple instrumentation to resolve and evaluate horizontal nutrient distributions within the euphotic zone for both ship-based and Autonomous Underwater Vehicle (AUV) based platforms in near real time. With these obstacles in mind three major goals were set. First was the development of a simple and robust chemistry that could detect nitrite and nitrate with a suitably low detection limit (approximately 10 nanomolar) and could also be coupled with a highly sensitive chemistry previously developed for ammonium with the same characteristics, and incorporate these chemistries into a single laboratory analyzer designed to monitor the surface distribution of these nutrients in the water sampled with a ships flow-through system.
590
Adviser: Kent A. Fanning.
653
Chemical.
Oceanography.
Submersible.
Analyzer.
Sensor.
Auv.
Ammonium.
Nitrate.
Nitrite.
690
Dissertations, Academic
z USF
x Marine Science
Doctoral.
773
t USF Electronic Theses and Dissertations.
4 856
u http://digital.lib.usf.edu/?e14.1093