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Examining the evolution of phosphagen kinases

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Material Information

Title:
Examining the evolution of phosphagen kinases a study of a dimeric arginine kinase from sea urchin (Strongylocentrotus purpuratus) eggs
Physical Description:
Book
Language:
English
Creator:
Held, Brenda Christine
Publisher:
University of South Florida
Place of Publication:
Tampa, Fla
Publication Date:

Subjects

Subjects / Keywords:
Enzyme characterization
Purification
Kinetics
Echinoderm
Sequence homolgy
Dissertations, Academic -- Chemistry -- Doctoral -- USF   ( lcsh )
Genre:
bibliography   ( marcgt )
theses   ( marcgt )
non-fiction   ( marcgt )

Notes

Abstract:
ABSTRACT: The phosphagen kinases are a family of enzymes that catalyze the reversible phosphorylation of specific phosphagens using ATP as the phosphate donor. The evolutionary relationships between the enzymes in this family have been studied for over 30 years and yet aspects of the relationships remain unclear. Here, arginine kinase from Strongylocentrotus purpuratus eggs was purified to homogeneity and analyzed for physical and kinetic characteristics as well as its sequence homology with other phosphagen kinases. The results indicate that dimeric arginine kinase from S. purpuratus eggs evolved from a dimeric creatine kinase after dimeric creatine kinase evolved from a monomeric arginine kinase. The molecular weight/subunit composition and sequence of dimeric arginine kinase from S. purpuratus eggs is more comparable with dimeric creatine kinases than monomeric arginine kinases. However, the kinetic characteristics of dimeric arginine kinase from S. purpuratus eggs, including the absence of substrate cooperativity, are more comparable with other arginine kinases than creatine kinases. These results indicate a unique evolution for the dimeric arginine kinase, as well as the importance sequence composition can have on the three dimensional structure of an enzyme and its kinetic characteristics.
Thesis:
Dissertation (Ph.D.)--University of South Florida, 2007.
Bibliography:
Includes bibliographical references.
System Details:
System requirements: World Wide Web browser and PDF reader.
System Details:
Mode of access: World Wide Web.
Statement of Responsibility:
by Brenda Christine Held.
General Note:
Title from PDF of title page.
General Note:
Document formatted into pages; contains 126 pages.
General Note:
Includes vita.

Record Information

Source Institution:
University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 001913699
oclc - 174145671
usfldc doi - E14-SFE0001951
usfldc handle - e14.1951
System ID:
SFS0026269:00001


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Full Text

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QD31.2 (ONLINE)
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Held, Brenda Christine.
0 245
Examining the evolution of phosphagen kinases :
b a study of a dimeric arginine kinase from sea urchin (Strongylocentrotus purpuratus) eggs
h [electronic resource] /
by Brenda Christine Held.
260
[Tampa, Fla] :
University of South Florida,
2007.
3 520
ABSTRACT: The phosphagen kinases are a family of enzymes that catalyze the reversible phosphorylation of specific phosphagens using ATP as the phosphate donor. The evolutionary relationships between the enzymes in this family have been studied for over 30 years and yet aspects of the relationships remain unclear. Here, arginine kinase from Strongylocentrotus purpuratus eggs was purified to homogeneity and analyzed for physical and kinetic characteristics as well as its sequence homology with other phosphagen kinases. The results indicate that dimeric arginine kinase from S. purpuratus eggs evolved from a dimeric creatine kinase after dimeric creatine kinase evolved from a monomeric arginine kinase. The molecular weight/subunit composition and sequence of dimeric arginine kinase from S. purpuratus eggs is more comparable with dimeric creatine kinases than monomeric arginine kinases. However, the kinetic characteristics of dimeric arginine kinase from S. purpuratus eggs, including the absence of substrate cooperativity, are more comparable with other arginine kinases than creatine kinases. These results indicate a unique evolution for the dimeric arginine kinase, as well as the importance sequence composition can have on the three dimensional structure of an enzyme and its kinetic characteristics.
502
Dissertation (Ph.D.)--University of South Florida, 2007.
504
Includes bibliographical references.
516
Text (Electronic dissertation) in PDF format.
538
System requirements: World Wide Web browser and PDF reader.
Mode of access: World Wide Web.
500
Title from PDF of title page.
Document formatted into pages; contains 126 pages.
Includes vita.
590
Advisor: Steven H. Grossman, Ph.D.
653
Enzyme characterization.
Purification.
Kinetics.
Echinoderm.
Sequence homolgy.
690
Dissertations, Academic
z USF
x Chemistry
Doctoral.
773
t USF Electronic Theses and Dissertations.
4 856
u http://digital.lib.usf.edu/?e14.1951