USF Libraries
USF Digital Collections

Physical, kinetic, and immunological studies of monomeric (Periplaneta americana) and dimeric (Isostychopus badonotus) a...

MISSING IMAGE

Material Information

Title:
Physical, kinetic, and immunological studies of monomeric (Periplaneta americana) and dimeric (Isostychopus badonotus) arginine kinases
Physical Description:
Book
Language:
English
Creator:
Wright-Weber, Brianne
Publisher:
University of South Florida
Place of Publication:
Tampa, Fla.
Publication Date:

Subjects

Subjects / Keywords:
Phosphotransferase
Enzyme characterization
Purification
Echinoderm
Quaternary structure
Dissertations, Academic -- Chemistry -- Doctoral -- USF   ( lcsh )
Genre:
bibliography   ( marcgt )
theses   ( marcgt )
non-fiction   ( marcgt )

Notes

Summary:
ABSTRACT: Arginine kinase catalyzes the reversible phosphorylation of arginine using ATP. This phosphotransferase is found throughout invertebrate species; whereas a homologous enzyme, creatine kinase, is found in both vertebrate and invertebrate species. Arginine kinases are found as monomers of 40 kDa or 80 kDa and dimers of 80 kDa while creatine kinases are found as dimers of 80 kDa, monomers of 150 kDa, or octamers of 320 kDa. The significance or advantage of the dimeric state or various quaternary structures is still not understood for this family of enzymes. Here, arginine kinase from Isostychopus badonotus muscle was purified to homogeneity and analyzed for physical, kinetic, and immunological characteristics. The results indicate that arginine kinase from the sea cucumber, I. badonotus, is a dimer with a molecular weight of 87 kDa that displays physical and kinetic characteristics similar to other arginine kinases regardless of their weight or subunit composition.However, immunological cross-reactivity using I. badonotus polyclonal antibodies shows that dimeric arginine kinase from the sea cucumber can react with dimeric arginine and creatine kinases but not with monomeric arginine or creatine kinases. Comparable results are seen with polyclonal antibodies raised against purified monomeric arginine kinase from the American cockroach, Periplaneta americana. Monomeric arginine kinase from the cockroach reacted with monomeric arginine kinases but not with dimeric arginine or creatine kinases or monomeric creatine kinases. Arginine kinase from the sea cucumber and the cockroach is substantially inhibited by the anion nitrate which mimics the transferable phosphoryl group in the assumed rapid equilibrium, random addition reaction. Here, nitrate has been shown to inhibit both the initial velocity and percent of product formed from arginine kinase in I. badonotus and P. americana.Difference spectra for each enzyme in the presence of varying components of the transition state analog suggest that nitrate has an effect on the enzyme itself and inhibits through a mechanism beyond that of stabilization of the dead-end complex. Further characterization of the dimeric state in these enzymes on a structural level included the elucidation of the protein sequence from the American cockroach and a comparison with dimeric arginine and creatine kinases.
Thesis:
Dissertation (Ph.D.)--University of South Florida, 2007.
Bibliography:
Includes bibliographical references.
System Details:
System requirements: World Wide Web browser and PDF reader.
System Details:
Mode of access: World Wide Web.
Statement of Responsibility:
by Brianne Wright-Weber.
General Note:
Title from PDF of title page.
General Note:
Document formatted into pages; contains 193 pages.
General Note:
Includes vita.

Record Information

Source Institution:
University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 001917741
oclc - 182554452
usfldc doi - E14-SFE0002057
usfldc handle - e14.2057
System ID:
SFS0026375:00001


This item is only available as the following downloads:


Full Text
xml version 1.0 encoding UTF-8 standalone no
record xmlns http:www.loc.govMARC21slim xmlns:xsi http:www.w3.org2001XMLSchema-instance xsi:schemaLocation http:www.loc.govstandardsmarcxmlschemaMARC21slim.xsd
leader nam Ka
controlfield tag 001 001917741
003 fts
005 20071128105104.0
006 m||||e|||d||||||||
007 cr mnu|||uuuuu
008 071128s2007 flu sbm 000 0 eng d
datafield ind1 8 ind2 024
subfield code a E14-SFE0002057
035
(OCoLC)182554452
040
FHM
c FHM
049
FHMM
090
QD31.2 (ONLINE)
1 100
Wright-Weber, Brianne.
0 245
Physical, kinetic, and immunological studies of monomeric (Periplaneta americana) and dimeric (Isostychopus badonotus) arginine kinases
h [electronic resource] /
by Brianne Wright-Weber.
260
[Tampa, Fla.] :
b University of South Florida,
2007.
520
ABSTRACT: Arginine kinase catalyzes the reversible phosphorylation of arginine using ATP. This phosphotransferase is found throughout invertebrate species; whereas a homologous enzyme, creatine kinase, is found in both vertebrate and invertebrate species. Arginine kinases are found as monomers of 40 kDa or 80 kDa and dimers of 80 kDa while creatine kinases are found as dimers of 80 kDa, monomers of 150 kDa, or octamers of 320 kDa. The significance or advantage of the dimeric state or various quaternary structures is still not understood for this family of enzymes. Here, arginine kinase from Isostychopus badonotus muscle was purified to homogeneity and analyzed for physical, kinetic, and immunological characteristics. The results indicate that arginine kinase from the sea cucumber, I. badonotus, is a dimer with a molecular weight of 87 kDa that displays physical and kinetic characteristics similar to other arginine kinases regardless of their weight or subunit composition.However, immunological cross-reactivity using I. badonotus polyclonal antibodies shows that dimeric arginine kinase from the sea cucumber can react with dimeric arginine and creatine kinases but not with monomeric arginine or creatine kinases. Comparable results are seen with polyclonal antibodies raised against purified monomeric arginine kinase from the American cockroach, Periplaneta americana. Monomeric arginine kinase from the cockroach reacted with monomeric arginine kinases but not with dimeric arginine or creatine kinases or monomeric creatine kinases. Arginine kinase from the sea cucumber and the cockroach is substantially inhibited by the anion nitrate which mimics the transferable phosphoryl group in the assumed rapid equilibrium, random addition reaction. Here, nitrate has been shown to inhibit both the initial velocity and percent of product formed from arginine kinase in I. badonotus and P. americana.Difference spectra for each enzyme in the presence of varying components of the transition state analog suggest that nitrate has an effect on the enzyme itself and inhibits through a mechanism beyond that of stabilization of the dead-end complex. Further characterization of the dimeric state in these enzymes on a structural level included the elucidation of the protein sequence from the American cockroach and a comparison with dimeric arginine and creatine kinases.
502
Dissertation (Ph.D.)--University of South Florida, 2007.
504
Includes bibliographical references.
516
Text (Electronic dissertation) in PDF format.
538
System requirements: World Wide Web browser and PDF reader.
Mode of access: World Wide Web.
500
Title from PDF of title page.
Document formatted into pages; contains 193 pages.
Includes vita.
590
Advisor: Steven H. Grossman, Ph.D.
653
Phosphotransferase.
Enzyme characterization.
Purification.
Echinoderm.
Quaternary structure.
690
Dissertations, Academic
z USF
x Chemistry
Doctoral.
773
t USF Electronic Theses and Dissertations.
4 856
u http://digital.lib.usf.edu/?e14.2057



PAGE 1

nrnnnrnn rnrrnrn nrnnnrn rnnnnnn nn n nnn rn!"!r rn nnnnn nrn rnnnrn nrnr#rrnnrnrrrnn nnn rrnn$rn $rrnnnnrrn %&rnnn' nnnnn (nr)nr&rn*+n,n++n n-&n++n nn++n .rn*n++n nnnn rnn&)n .n/0n1223 nnn r4)nrrnr5rnr5 nn#rn rnrrnn n 6n$n1223nnrn!"!r rnn

PAGE 2

n nnrnrn rr rnrr rnnnrnrn nnrnrr nrrr nrnnrrnrrnn nnnnnr nrnnnnnrr rnn

PAGE 3

nr nrnnn nnn nnnrnn nnrrnr nnnnn rn nnn r !nrn"#nr #r$rnn rrnn%n& nnnn rrnnrrn%nn rrnr n rnr nn nrnnnnn nrr '()r*nrr nrnrn% rn rrnnrr n &nn+() n$nr #nnnrr nn )rnnnrn nnn nrnrn%n nnn,n nrnn &n

PAGE 4

nrrr rr rrrrrr rrrr r

PAGE 5

nn nrrnnrnr !"n!# $ %&''()'* +'$,!$!-r .& $(/ .))(+ $ (+ "'(+-% (+$"$01--2. 3r1-2.

PAGE 6

%$ 'n *2(2()4$n' 22 'r2& 56''2&5$n 2& 27n$'27"'27-'n 28r285 n%*n28 n 4 9 $'n*+ n + -49 + 49+. :* $'n *+( +(-)r+2 3n++ $n$ ++

PAGE 7

"$ ++ :%;* +7 ;+7 n +749+8"$ &< :)9 & ) & 3 & r &. "31&. "3' &/ "' &( $, &2 $,' &2 '"3 &+ &+ "2=/=' && 2=/=$, &8 $,' &8 / 7 % 7

PAGE 8

r 7 )4$n' 7 'r 7. 56'' 7. 5$n 8. 8. n$' 8. "' 8& -)n 8& r 87 5 n%*$n 87 n 4 9 $'n* <. n <. 49 <. $'n *<& <& -)r + 3n 8 $n$ 8 "$ .(

PAGE 9

r :%;* .+ .+ n .+ 49 .+ "$ .& :)9 // ) // $, // 2=/=' /( 2=/=$, /2 $,' /& (" /8 % /8 '' (&* 2.:' -r +. :+7 &/ n

PAGE 10

n nr n !"n#r$% &'r ()* +"n#r$% &'r ,* -n.r ..r/0 0nrr. 1, )*r 2+ 3n* r..r/n&$ 4 ..r / 5n...r 2nr$.6. .r ) + nrrr.1, )$r*r nr$r7*r7 *r *#r$% --

PAGE 11

n nr nr n !""#$r "% &n!r'"# ( )n#"*"r# +n*"r"rr, $" -n*"'# + %n#r" r(nr. /0& 1n#r &% n "2# r&( n "23,"r.24#*# )1 n*r'#5 r) &nr#r46 r) )n5r r"#)+ +n##&" %& -n'7" %) %n*2#6 "r" rr%-

PAGE 12

(n$r8*"4 r"#%% 1n$r 8#%( n."0r (1 n'9""##r5 *r( n'#" r"( &n0rrrr"# (& )n#r"r"# () +nr*r"r"r" #(+ -n rr$r" r,r#11 %n'r": r#1 (n8;r" #1 1n8;$"r *"r#1) n8;*"r 1+ n r0/ r1( n r#*0/r 1 &nrr )n r32#0/r +n r#.0/r -nr5*r & %n r$#r" #(n r4r r"#1

PAGE 13

7 &1n0/r#/ r 449 &nr0"4 r"# &n*rrr"# r4) &n8;r,r# ( &&n8; 1 &)n*rrr,r# r4 &+n*rrr,r# r; &-nr./# ";/0 % &%n" rr *" )&(n4r!r# "#"r r,r"#" #rr+&

PAGE 14

nr nnrnnrnnnnn r! r nr "rrn"nn#n!#$$nr$n!n#%r&%rnr"! "! n$$nrnnn$nr

PAGE 15

$'(nr ))nrn)) n*+,,!nrnn n r-n.!nn nrnn // n//r!n##n rn##n rn "n r"n,01( rn ""nr "2"nr n3 45 ","nr n 3 5 "n. r."n"0 r4 "n"1( r""n"10 r"n

PAGE 16

"n1, r,"n" rnn"."nn*.*rn )$nnr)$n **n+rnn+n.nn .. #%r& *% 6%% +"+! r *n$n$nrnnn3r5rrnnnnr6.n6

PAGE 17

nrnr r n r rn rr nr n nn !n "#$n $%%! n !$$&'()'( !!$)'(# !!$)'(%$$ *+'(%$,-'( n!n!$ !!$$ /% n$ nr $#!$n!! %%! $$n n$ $% nr %!$#$#n)0( !$ nn! #n$ /#%$$n rn nr !#!$n $ $#!$n! #$$ n $ #! !n!$$n $$%

PAGE 18

r r 1$n$ !# $$n#!$n $$ n$ $! !# $$ n$!!.$%!$!! /% #! !$!$n nr r ($ n$ nnn $!n $$! !!r!$ 2 !$$ !!! .$$ !$#!$n

PAGE 19

nrrn rrrr r r !" #$ $ $$ $$ %!" rr r &$ '"$(()*+, '-.$%///!" r r0 1 2r3.r 4"$(56!" 78r 0rr "r

PAGE 20

% r'9:4rr:4.r:;: "n
PAGE 21

= %">r#8r!$
PAGE 22

6 r r+"$((%! "r .r # r#r:4.r:;::4r r#4r4.r:r:;:@#:r r1 2 r" r! ;:"Ar + "$((%!"A(5$8 "> &$$ r"$ %//=!" 124"$( 56!"$ $ rB,8$(5* B,<$(5C!".<' D"$

PAGE 23

C (E6!$r$ !$r $ r.r;"$ ((C!"78rr" <& "< +$()=*4$()=!";$ $$ $$ +$()!" & $ n$(E6*,n$ (E(*n()6*?, '$((5!" #! 0 ,F$()(!$<' 78r ?$%//!*! *! ";$

PAGE 24

E 0F-r"$( )%!" $ $ -? $%//!" ;! >, >$%//6!"G 0$ $ 6/. 5/."A$ $$ "$(()*',4 $((5*B"$%//6! >,8$(55*+-+" $%//E!"?& C/.& n"$(E(! -5/.> "$((5*>, H$%///*>"$%//%!" 0 "< &&";$

PAGE 25

) "F $<'-44 !$<'-BB!$<'-4B !&5/.? "$(E)!<' =%/.+ "$((/!"G$ $ $ C/., >$(5C*"$(5)!"rB (%/I $"G '"(C6! <' ?"(CE! '"?" & $ r"$(E6!$ B,'$(E5!$ "$()C!$n ,<$(55!$8"$ (5E!$?$%///!" 0$ $

PAGE 26

5 $&$ $" '<' "r! n(E)! .r! ;8$(5=! J "$%//C!" $ 6/.5/." $$ $ >.>-r8?$()/!" & $ $0 !" C/K /"4<'44 C/K /EL<8,4$()(!"4<'-BB$ '$'

PAGE 27

( C/KC/L<$C/L<$65L<8,4$ ()(*B"$%//6*+-+"$%//E !". $ nr $C/K=5L< $ n nrr $C/K%EL< %/L<$>,8$(55*+-+ "$%//E*n, <$(55!" $$ nn r n nrr " & A!" ; & $' ',4$((5! <'? "$(E)!" %!"' $ nr $

PAGE 28

/ AC"5+-+"$%//E! 0$ nrr $ AC"=>$())!";$& &<'-BB$ n $6"5 AE"(<'-44 8,4$()(!" <'5/K 4"$((6!$ 44 ; ; "? ; ;C(" ; 'I ;,>$()=!" ; r!ME"E)"% .r!M5"6("4$()=!">$ ; ME"C)"/ M)"C("C+$()=!" ; ";$ J ; ;"

PAGE 29

">< '# &C/K /" L 4<'-44 nn E 8,4$()( 4<'-BB nn C/ 8,4$()( ><' nrn =5 >,8$(55 >N?' nn %E +-+"$%//E >N?' nrrn %/ n,<$(55 <' nr C/ B"$%//6 B' nrn 65 +-+"$%//E >' nn =) "$(()

PAGE 30

% %"ArA!< '# 4<'-44 nn E"( 8,4$()( 4<'-BB nn 6"5 8,4$()( ><' nrn )"5 >,8$(55 >N?' nn E") +-+"$%//E <' nrn C"5 +-+"$%//E >0' nrrn C"= >$())

PAGE 31

= & 0$<.! $& $"< 0 0 <$(56!"<. & &" n<')"5K$ %K$%6K44$BB$ 4B$8,>$((5 !". $ r $=K8"$ %//=! $ nr $$ n $%KEK$ +-+"$ %//E* ,8$((E!" 0$ $0 0 "A <' "<'-44 <'-44! ?"$ (E)*8,4$()(!"

PAGE 32

6 '<' (55 >8 ";$ 0 rrr $ & "A 0 "n !"A()/$F "" $ 0$ & "n" ()E!F n

PAGE 33

C <' &"<<' '" <' 84()("r <'-44<'-44$<'-44$ <'-4B <'-BB" 0 <'-44<'-BB 0 "4 <'' B"$%//6!" '<' -"<$

PAGE 34

E !"# A<' (C/I '"$(C6!" 4%: 4.r4 r!" 0; 5"/$$0 $ 4,D$(EC*4,<$(EE* 4,+$(E)!" =! 0& &>$()C!";) "/$>< ()=! & 0 '' (C/I(E/I &4 "$(C)*8"$(C)* >,4$(E(!"A N"$ (EE!";$0$ F"$(EC*GI> "$(E(*B$()%*"$()%!" <'$

PAGE 35

) +$()=!" $GI>" (E(! & 4& r B$()%!"> <%:$O%:$<%:$4%: 4$()=!" <'' r!r! =B6B!" &" $'$&P?>Q' &P?>>%Q"$ ''$ '4'4$' "'4 '4 >$()C!"

PAGE 36

5 ="n?0n4 # r0 $ 4.r M r ?Mr ?4.r 4r Mr?4.r R?4r 4r Mr 4.r ? ? ? 4 r

PAGE 37

( <'-44<'-BB$$ 'r /"((="%E4'/"==/"5(4 =!4,D$(EC*; "$%///*<"$ %///!". E"/6C"E4 &6"E=("C4 &=!"6 <'44<'-BB "'.r/"/%/")4' /"/=/"/C44,D$(EC*+ "$%//*+"$ %//E!"& /"%% 5"E4& /"C%"(4" '4 &-$ ;$ !$ $ =B6B &! &''"/4>,8$(55 *N, >$%//6*B,8$%//6*" $%//)!" $$

PAGE 38

%/ '' "'.r/"/E/"%/4 $$0'/"/E/"/(4>$ ())*B,8$%//6*;"$%//)!" "/="C4'/"(6"=4"G '4 " "A$

PAGE 39

% =B"'Q&P?>>%Q" "# "# "# "# ><'"="E/"C")>,8$ (55 ><'/"5%"6E/"6"%CN,>$% //6 <'/")/"6/"6C/"6(B,8$ %//6 B'/"(%/"(C/"(("/"$%//) "# "# " n<'-44"%/"65C"EE"4,D$( EC <<'-BB="%E/"==6C"E6"E=;"$ %/// ;<'-44"%/"5(6"E("C<"$%/// ;<'-BB/"((/"5E"/6"(<"$%///

PAGE 40

%% 6B"'Q&P?>>%Q" "# "# "# "# >N'/"/E/"/E"=/"=;"$%//) <'/"%/"/("///"(6B,8$ %//6 >0'/"%/="C>$()) "# "# " n<'-44/")/"/C5"E%"(4,D$( EC ;<'-44/"/)/"/==")"==+"$%// ;<'-BB/"/%/"/6/"%%/"C+"$%//E

PAGE 41

%= A $'3'$ 12 >$()C!"> "> ()C! "r '3'" $ $ +$()=*D,'$()/*< ,+$()C!"G $ ' "$%//)*B, 8$%//6*F"$(EC!";$ $ nrn nr $& "$()C*>,8$ (55!"A%///$;" "G 4,D$(EC*@ "$%//6!"

PAGE 42

%6 0 "> "< $ +$()=*4 '$%//C!"N$ -"? r $ n $ nr $$.-n "$()$B$()%*B ,8$%//6!"? <'@"$(E*' "$()/!" -& 4-+,+ $()!"$@4n$ $S-0 -& $$<'--4 .r! -& $$$<'-4.r-!n, <$()%*n,$(5/*"$%//%! "4-++()!

PAGE 43

%C <'>@% $ $ !">$ & @4n B,<$()6*"$()C*B "$%//6!" 4-++()! <'$ $#;G6 %-$ ;rG6 %-$
PAGE 44

%E J -&" n"$ ()/*n"$(E5!"0 "n"()/! $ TU$r.r$ $ $$4r4.r!" r & +$()=*,+$ ()C*"$()5!"<'-BB&<'44 %E/ -4.r-!";$ ,+$()=!"8 8-n(5)!<'-BB &">$ ,+$()C!"

PAGE 45

%) ,+$()C!"A$ #r#$rr %&r r <' 6/. 66.4"$ ((6!"&5/K0 $ E/ECK0 4 "$((6!"A && <' 0 @??.-& !$ ?"$%///*<"$%//!"0((6 > ">$ 0 6/K0 >"$(((!"A$ nr

PAGE 46

%5 0$ C5-E%K! <'0 ==6CK!>"$(((!">$> "%///! &-E)! J' nr <'-44" >0> C'$'$ <'" r 3"A %//$<'0 0 0%( E 08B ?"$%//!"C= K<' =K' & 6!" 0)E <''0&

PAGE 47

%( 6">0;< '# 0%(E 0 <'! '!"< !V(/K$ !"B# <''*n# <''*8# <''" <''0 ?"$%//"

PAGE 48

=/ + $ ">"(()! 0 08>! "8> "8 $<' $''C $ C!"0 $ r $ &8> >"$(((!" 8>$N >%//6!8> nr '" 8>F& -" 8> rE=EC 8 '4

PAGE 49

= C".'<'? # n#*8# *B# *r# ;$%//)" >0#.-W55C56%)W*>N> -W%C=/%W*>
PAGE 50

=% $ & ?"$(((* -+"$((E*"$%//%!" ((E -+" 4-<'! @r" @%!<==5/!r E!"$ E/EC=E=%E$ & & 04"$((6! "r 3 r &0 n"$(5*<" $(5!"S%XTr $8%%E8%%)$ $%%5$ "r CX TC!" <'-44 AE(F=%C

PAGE 51

== E"n.><< '4# &r @< 4, '$%//C"
PAGE 52

=6 8%=%<%5="$ %//%!"$%%=$ F$"()(*4,'$(5C*8 "$((6!"$< %)5$ Tr 4"$(E%*+" $((=*B"$((%!" r ((5O" &" $' @<)!"$ E=-E5=/(-=5$ r '$$ $ r > E=$8E6$FEC$E5$8%%C$<%)$8 =6! C .r" <' -+"$((E!" ?"$ %//!"A & O"$((5!">E=$8E6$ FEC$E5

PAGE 53

=C r nr 'N,>$%//6! -0 N"$%//E!" '<'0 '<' 5!"<$ ";$ 4-<'' "!"A "4<' &><$ "$%//%!"

PAGE 54

=E )"n.;< '# @-<" &-"4.r# *@#*# E=-E5!=/(-=5!" O"$((5"

PAGE 55

=) 5"'>> <'># rB.n( "$%//6! r.BFnr"$ %//%!"

PAGE 56

=5 <'<' "N> %//6!E%$8> $(=$$ "A $ >"$ (()*<"$%//!";$" %//6! "<$ & O"$%///* "$%//6!" =/ 0"A #! *! *! *! *! 4 ,+$(E)*F$

PAGE 57

=( (E5*+$()!" "()C! "G $$ 0 4"$(E)! n"(5(! "? n"(5(!

PAGE 58

6/ 0$ 0 $ nr $0>"(((!$ $$$ $0 & -! 0%J"G $ $$' r ">"(((! $'<'$ " ?%///! <' "r $ n, 8$(5/!"n$ nr ?*<' & $ 0"? %//!'<' J "?<'

PAGE 59

6 8''! <' $$44$BB ( $$ ;$%//)!" nr "A $ r"%///! nr 0' A "> '<' <'" <'!

PAGE 60

6% ("rn FA# " ; $%//)" r#4 >#.'$.<' #4'$8'$'$;'$'$' 4#-.'$.' A #4' >N#.<'$<'$.' ><#.<'$<'$.' F#.<' <#4'

PAGE 61

6= >(E/I B,>$(E5!"> $& O"$((5*H"$%//!" $ rnr $ 8$ !nr "A B "$%//6!" I
PAGE 62

66 B"$%//!">$ nr $$ +;G3A NA > 8, >$%//)*>"$%//E!" ><' $ + "$((%*>"$ %//%!"4$<' $ +"$( (5!"A%///<'BB-& +, '-.$%///*+, >$%//%!">$ <' "A <' $$ J>"$((5! 8"$%//%!" $ 0 ".

PAGE 63

6C 0>"$ (((*>"$%///!"< 0?$%//!". 6/$ 0 "@ 0 $$ $$ 0 "<& $

PAGE 64

6E 'n!!rrA">r rr B()E! "r 4r .><<>" $4G!"B )%C "%# $ -r /!$!n(E)! "&/"C! /4$"%C4.r$/ "64@.r$/"CN3 &$N3-E$/4$=4 $%/4/"4 3;<$;5"/" $ =6/ B<.NE6/> $
PAGE 65

6) $(5( !' %!" &/4 $4.r$/4 $%/4$/"64 @.$/4.$/"CN3&$ N3-E" @. 6/C /!$.r$ ; =!$nBN (/<$A!" &%!%"/4 r$C4-$="/4 $%"/4J ;5"/". %CL<"/"/4 @G; ;
PAGE 66

65 nrn nr n n r n n r n n n n r 3;<;5"/" =6/ /"/C/" /"'n#

PAGE 67

6( "?-
PAGE 68

C/ %"?-<-@. # #$r% &"nn n n r n n r r r !" r r r r r r n r n n n r n n n r r n r n r r r r r r n r n n n r n r n

PAGE 69

C =".;> n # rrnn n n n n r r n n n n r n r r n r r r r n r

PAGE 70

C% (#)%%rr r >.>! ()/!"> %"CK 6K"r $ 0 /"C43;<;E"5$/K >.>$/4%-$%/K$%K C"? %C43;<; 5"=$(%4$/"K>.>" C/! "r B-n$n$.>-r8? >.> "8 6! =/4 !$%4.r$%/4$/4 $%4 @.r$%N3&$"EN3-E$%% 4 n $/"=E4 %C4

PAGE 71

C= 3$;)"C"$ )/K-CK =/"> ><'=! !" 6"<>@8 ?# rrrrr n n r n n r r r !" r r r r r n n n r n n r n n r n r r n n n r r n r r n r n n r r n r r n n r r r r r n r r

PAGE 72

C6 rAA"r<>< r 8 ><$ #n 8>4$A"$r$ !$ >8(55! rnr "=/-C/ C/43$/4%$4?.$%C 4 $C/ $ @@=$ /"!nA$A@!$;5"/ +" 6$C//&%C 6L< "5$///& 6L< $ C/K $ /$6$/// &%/" 5%K 6$///&%/" C/-5%K J'" C/43; 5"/$/4%-$ 4?.$%C 4r4> )"C <=6<$ $
PAGE 73

CC ;)"C"6 &%")!;& B-n$n$&8-//"( &C="=!C/ 43;<;5"/$/4%-$4? .$C 4 n @@= "3 44! !" >.>-r8?"4%/.!$-= .!$ 5).!$6%"%.!$ =%"=.!$ 5"5.!$5"%.! Y'"

PAGE 74

CE C" < '# '()(* n r n r # $ !r n % rr $ !r n % rr ! & n # $ !r n % rr ! & n # $ r # n rrrn ( ) nrr ) % $ ) r n rrrn ( n

PAGE 75

C) &r r -C$ $ -",; -@. %! ">'% Y!C & "/ "43; 6"CE"/$/"4 ;E"C )"C$/"43;<;5 "//"/"; ; $; &12 ;" ,; /"4 $4?.$%4.; E"6-5"%C" /"%!! "

PAGE 76

C5 r.r An rA? $A"$ $@D! 0Z\%%%:%=6/3=/$=//<"()%!"

PAGE 77

C( %rrr '/"/C!'$ rnr $/"/C! <' "&r '/"! 54 /"!/"43;<$%4.$;5"/C 64" =/ !" /"%4" '/"!/"! ",rr #" r=Y! =EY!$ nn 4>$88$
PAGE 78

E/ & ">! '$!'$! &'$ !&3 '$!3 '$! 3'J 6L<"8/K 6K $66L
PAGE 79

E rAAA"rr+ B><' rr rr#r r' r>$A" r$!" 0-5/L<" rr %/ r+0r# ;& /"%C/ "/C43;<$/4%$4?.$C/[4@@=$%C[4r4> $;5"/" ; %%$///&/ 6L<"r "r+ $ /"C/ "@ &-$$ $$$

PAGE 80

E% 8 >4A"r$ !"> %n# !$ n >>!$ rnrr !$ &rn r!$ r r B!$ 'rnr !$ &nr !$ r (rr B!$ n#rrr >!$ #nr .!$ rn B!$ )rnr >! *rr(n B! "n# 8> .$ 88 $.>" J"8 /"/C43;<$/"(4 $%/K$;5"=/$ $ A-BrF. B-n $n$
PAGE 81

E= 3/"/%4 3;<$/"C4@<$;)"66/ %-=/"K3! -3"A #%$///!K-3 /"%K3! %/6C /"K-3 #%/$/// -A8J !K -3 ". C//"%4 3;<;("CC $=")C--6--=-.4 $"%4 "

PAGE 82

E6 rAF"'>< "# -" &/"C! -/"C4]="/4!$ .r/"%C4]="/4!$ /"==)C4]5"/4!$%/4 $%4.$/"64@.r$ N3-E-$N3 &/"48-8 ;E")C" %").r .r6$C/ 4-!4"$(E!" ; & -/"E4]% 4!$r/"/E%C4]/")C4!$ "/E%C4]=4!$ 4&r".& -B"n & &! F(C )!" $00?0 $%$=$6!4$%//=!>r5 "/B -" ?

PAGE 83

EC B<&n +' #r & PQ +! #r & P%Q <&n +!' ' & &P=Q +'! ! & &P6Q % r !rr&#rr -& '-4.r-!" -& ; C/4 #@<$@A$@><@$@@G%$@@G=$@<;=
PAGE 84

EE *r & -& !"A @G=-&$ '" ="%C4]C/4! ; " ; -.$$-$$-$$ /"" rr -&' "r

PAGE 85

E) /"/53!/"43$ 4?.$%4.$; 5"/ %//-E// %CL<"&/"(!/"%4.r $"%4 $C4-$C/4 ;J5"/" /"( /"( I" & & & B<.NE6/ >"

PAGE 86

E5 rF"<<' r1r r rnrn B"%//6!"> & 6$C//&6L<" C/L&8-//$6&)/! & >&.??-C/$%"C&6"C! /"=4@<" rr#rrr r "%! r>$A"r$
PAGE 87

E( +0r+ %"CK$6K! /K>.>" "8 /"/C43;<$/"(4$ %/K$;5"=/ $$ A-BrF. B-n$n$+ rnr !$ n >>
PAGE 88

)/ &rn r!$ rn N!";& + "r /"C/ +" rr -& "r /"%)C3!/"4 3$4?.$%4.$;5"/ %//-E// %CL<" &/"(!/"%4.r$"%4 $C4-$ C/4" ; J5"/" /"( /"( I & & &B< .NE6/>"

PAGE 89

) rFA"4B r!< C" -"F "+ % r" %/ &" $"G$ &// rrr r*# An@ n@-B-$A" $S!" C/! n@-B -"
PAGE 90

)% )CK$&$)$C//& C6L<" n@ /" .?r5[.@$%[S?$ %[r$ 4$+A!".@//!r4 $+A!

PAGE 91

)= *#.r rA.@$ >"@""r"8r 'A<$E"E4! $&$ 6%-C/L<" B)43;< !& "&>"@""r" =$///&=/%CL<"0 =$///& =/" .@" 0 /" 6S +6//4 @/"/6! /$///& .@".@ -%/L<" Ar
PAGE 92

)6 $///&C %CL<" /"/% "$ $$///& %%CL<"r#33""3 3 !"B 4#33""33 !" 0 <$.$?!" 088@>+@88n< -=I

PAGE 93

)C $r Gr/< G><< )nr =/ "-=/ 6%L< ">"G"<"/"%C! %// !=)L<"/-C/[! B C/[3[! =)L<"< =B3 =//!"

PAGE 94

)E rrr *#A.@ ?+$@H!rr4 "B"C! % $///&%/" /"> &/"> % ">=/" !& "% $///&=/ ".@B 4&/"6C! $&$ %$///&=/" /"6.r > %$///& E/" &r> >C/[! .@"r .@" "/[;$/"[B>$ /"%[?nr$4$ +A!$6")[$E[.@ /[" =)L<6.@ "04 B<

PAGE 95

)) 4
PAGE 96

)5 n@ECL0 CI=I $0$ n!"1 2 0' nr $CI=I" 8nCI#CI-<8<888<<888<<8-= I 8nCI#CI-88<<8<88<8 888-=I 8n=I#CI-8<8<<8<8<<8<8 -=I 8n=I#CI-<8<<8<88<8< 88-=I

PAGE 97

)( 2343$?0CI=I r"@""r"4'$ 4B< 40 @
PAGE 98

5/ "$(()!4?8'"$%// 6!"@J 40 "B C/K"

PAGE 99

5 4nrA"r< r8> <$ "n # $ rnr >,8$(55!" E!" ;& )!"C =/-C/ "C)$%K$5"5 >.>-r 8? 5!" !r+rrr >&8-// 44! !&5 /$///6/$///$

PAGE 100

5% "@ (!" >.>-r8?" %/!" & 6=$)//" "n# & 5)$/// "&r %CL
PAGE 101

5= %%!" ; &&;5"/ ; &&;E"/"n ;

PAGE 102

56 n r r r r r r r r r r r r r r r r r r r r r # $ % & '( )* ( + r r r r r r r r E"<=6
PAGE 103

5C n r r " " r # $ % & ( ) *+ ,+ / r " 0 % ) ( 1 2 % ) 3 4 1 & ( 5 ( & 6 r )";&
PAGE 104

5E C"r'> <# B ()E!" r r 5r PQ r r PQ r #& PY@.r; :;:6 Q #& P3Q .r % 7 8 ; %CC %=)("( E5"6 /"/= --// ;>< %C %C)"E EC"= /"/C "5 (C"6 >r )"C EC"6 ("( /"C C"= 6"E 8 <=6! 56 =") %"% /"EE %="% =/"( <;&! CC 5"5 6"C "EE C)"E %

PAGE 105

5) 5">.>-r8?n> >.>-r8?" #'" %#" =#N"6#> "C# <-=6"E#< <" )#<;& '91 421 921:14'191 ;1 4: 2 < ;

PAGE 106

55 ("4+.@ ><'# '/"%3! >&8-//"( &C="=!C/43;<;5"/$ /4%-$4 ?.$C / 4@@=" 3 44! >!" 4_!">< `!". a!" n r r r r r r r !" # $! % r & r r r & r r & r r & r r & r r & r r & r & r & r & r & r ( ) + ) / 0 12 3 4 5 6 7 6 8 59 r & r r r & r r & r r & r & r r & r & r r & ( ) + ) / 0 12 3 4 5 6 7 6 8 5 9 r & r r & r & r & r & : ; < ; = > ? @A B C D > E F G A G = H > ? B C I @= > ? @A F C

PAGE 107

5( %/"<<>4 +'# >.>-r8? $()/! n r n ! # $ % & ( & ) # $ % & + n & ! r + n # $ % / ) + n 01 $ % / n + ! r & 2 n # $ % 3 1 # $ % 3 4 2 # $ % & # $ %

PAGE 108

(/ n r r r %"A#r $ $ _!"C^!"/`!" Cb!"

PAGE 109

( n nr %%";G' n.# _! ;; & 12;" !-@. ">' % / #/" 43;6"C-E"/!$/"4 ;E"C-)"C!$/"4 3;<;5"/-/"/!"

PAGE 110

(% ,;' /"4$4?.$%4. ;E"65"%C!" & ; ; %=!" G-$ &%CK ;%E"r.r; =-/! E"/ % 6! AE"/AE"C '" ; nA?)"C&/="#rrr' %C/E// %C!"

PAGE 111

(= n r %=";>' ><# ;'/"%! /"4$4?. $%4.; E"6-5"%C!";E"6ccc!";E") ccc !";E"55 ccc !";)"/ ccc !";)"= ccc !";)"C ccc !";)") ccc !";)"( ccc !";5"%C ccc !"

PAGE 112

(6 n r r %6"A ><'# C/CZY @.;:;:3!' K;=-/!E EL<" ;n A?)"C&/=" _!";d!"

PAGE 113

(C n r r r r r r r r r r r r r r r r r r r r r %C">><'#'/"%%3!/"/C4 $;5"/ %C/E//"

PAGE 114

(E n r %E"<.>>< '# /"/)3! %4$4%$;5"/(/ %6/"4 %C

PAGE 115

() r %%% %/5 %E!"N <"()%! (K" $ <. "A $<. %rr!r@ %) !" ' nnr <'A!"> <'-44A)! <'-BBA6!" A$$ <'-44 <''"

PAGE 116

(5 &r5 4 C"@ =/ "& "A &$ $"A $ ,rr #"r n !& E4" J %5!"?& & 3 &-& 3' '" 3

PAGE 117

(( 0 $$ 0"

PAGE 118

// %)"?4>< <'# >/[! rn r !%/[! %/[!= <'A!" @8? 4"#A<'" %#<'"=#> <'" "(!!"(!0 "(00 '4

PAGE 119

/ 4: 2< %5";><>N '# r'=[!' =E[! =4! =/" &$J @8 ?4" 6L<" #@><'"%#@> N?'"=#4& @><>N?'"6# 4&.3n> <'>N?'"C#. 3n>N? '"E#.3n><'"

PAGE 120

/% rAA"rr+ B><'rr rr#rr r>$A"r$$0$-5/L<"r '"n# J>.>-r8? %(!" +0r%= E!" '' $ =/!" $ 5/."> <' n r 44BB!& <' =!"> -$ <' '"

PAGE 121

/= %("+Bn>< '# C[!J>.>-r8? 8 ><' -+" #%$/// #%/$///" .' .((6!"#' "%# 8><'"

PAGE 122

/6 E""$%//%!"4C/.n "$(5(!" nrrr nnrr+ n n r rr r r n r r rr n r rrr r r r rrr r rr n r n n # n r rn n $ % & r r n r rr % n r rrrr r r!r r r"rr ( r r!#r r rr ) % r rr ) % r r%rr n n n r +%n n $ % n r ,r$r%r r rrr % r r

PAGE 123

/C '4: 2 =/"+Bn4 .'# ?&/"%C /"/C43;<$ /4%-$4?.$C/[4@@=$%C[4r4> $;5"/" ?&%%$///&/ r+$ /"C/ ">C[! J>.>-r8? 8>< +Z# %$/// Z#%/$///!".'3<' .((6!" #>'"%#>'"=#> ><'"6# 8><'"C#'

PAGE 124

/E 4: 2< ="+Bn.< '# ?&/"%C /"/C43;<$ /4%-$4?.$C/[4@@=$%C[4r4> $;5"/" ?&%%$///&/ r+$ /"C/ ">C[! J>.>-r8? 8>< +Z# %$/// Z#%/$///!".'3<' .((6!" #'" %#8><'" =# &r !<'"6# r !<'"C#<'" E# <'"

PAGE 125

/) rAAA"'>< "#$$ 0$>,4 $(E(! =" "n $& 4.r4.r & -$=%==" &P?>Q'!-4.r "//4/"C/4$" =6 '!&P?>>%Q F(C)!" '-"//4'4.r/"==4" n $&4r 4r &-$ =C=E"'-4r/"=E4/"/=64$ '=)! /"=)4-!/"/)44r!"

PAGE 126

/5 )" $'3'$ & 12"> !"'3'' "// "C-4.r$ "'3'' /"()/"6(4r$" $ &$ & $$ '"

PAGE 127

/( n r r#$% r =%"?rA F# -& 4.r" %") .r .r6$C/4-!4"$(E!"="/4 4.rd!""/44.r_!"/"C/44.r7!" /"%C44.r^!" /"%/44.re!"/"%C44.r`!"

PAGE 128

/ n r r # $ % r r =="?.rAF#4.r& -" %") .r .r6$C/4-!4"$(E!"="/4Mr d!"%"/4Mr_!""/4Mr7!"/")C4 Mr^!"/"C4Mre!"

PAGE 129

n r =6">rnn# =%== F(C)!"Mr4 _!"4.r4d!"

PAGE 130

% n r #$ % & & & & & & =C"?-AF # -& 4r" 4&r "/")C44r_!"/"C 44rd!"/"%C44r^!"/"%C44r7!"/"/E%C44r `!"

PAGE 131

= n r ! =E"?rAF#4r& -" 4&r "%"/4-_!""/4 -d!"/"C/4-^!"/"%C4-7!"/"E4-`!"

PAGE 132

6 n r =)">r # =C=E F(C)!"4r4 d!"-4_!"

PAGE 133

C )".<' ><# .&4"' &P?>Q' &P?>>%Q"'3' 12 ". 004$%//=!> r5"/"?0 44" &r 'Mr Mr '3' Mr 4.r 4.r '3' 4.r "//f/"= "//f/"/5 "// /"C/f/"/6 /"==f/"/= "C .r=r '''3' 4r '4r '3' 4r /"=Ef/"/E /"=)f/"/6 /"() /"/=6f/"//5 /"/)f/"/ /"6(

PAGE 134

E % r !rr&#rr 0$ -&$ $'--4.r! $$$ '--4.r-!" #@<$@A$@><@$@@G%$@@G=$@<;=
PAGE 135

) n n r n =5"?4>< '# r' ; C/4 /"/C"@ cccccc!"@<;=<@ cccccc !" @@G% cccccc !"@@G= cccccc !"

PAGE 136

5 5"?4A F><'# ' 0 / /" // r 7r>(?r#&r>@?r 5r>5r? @<;=<@ -E @@G% -)E @@G= -55

PAGE 137

( *r. C/4! $ '" =(!$ $ 6/6! .r" $ -& $ & -&" N$ -" .-$$$-$-$$ "-& CK -(!" .-$$$-$$

PAGE 138

%/ n n r n =("?@<>< '# r' ; ="%C4]C/4!" /"/C"@ccccccc!"="%C4@@G= ccccccc !" E"%C4@@G= ccccccc !"C4@@G= ccccccc !"%C4@@G= ccccccc !"C/4 @@G= ccccccc !"

PAGE 139

% n r r r n n r r 6/"AF'F <@@G=# ="%C4]C/4! "

PAGE 140

%% n r n 6"rA>@ ><'# ' ="%C4]C/4! "' 0 //" //"

PAGE 141

%= (">>>< '# ; -C4!.-$ $-$-$$$" /" -" 75r // .-< C -; -G < 8

PAGE 142

%6 rr "B -&"A $ 6%!" $%)/ =//" %C/=/" "

PAGE 143

%C n r 6%".>><' r@#r/"/53! /"43$4?.$ %4.$;5"/ %//-E//" &/"(! /"%4.r$"%4$C4$C/4" 44"? -cccc!" ? cccc !"?$-$ cccc !"?$-$ $.r cccc !"?$$-$$.r cccc !"

PAGE 144

%E rAF"<<' r$ rnr $ B"% //6!" 6=$/// >.>-r8? rr#rrrr $ rnr.n r>$A"r$$0$ -5/L<"r rn r J>.>-r8? 6=!" +0rC

PAGE 145

%) $ '"n$$ $ 66!" rr $B 8$%//6!"A '"A C/4$ '(%K5(K /B"$%//6!"G $& -& '=/K CKB8$%//6!" '" & $%)/ =//&=%/ 6C!"F && $-$

PAGE 146

%5 $ $$4.r!"A$ J" "G-$ $ &" 6E! %5/-%(/ J" $$ =/K! $$$4.r! $ &%E/" &"

PAGE 147

%( 6="+Bn< '# C[ !J>.>-r8? -+" #%C/ #C$///". .((6!"#' "%# '"

PAGE 148

=/ 4:2 <; 66"+Bnr' # ?&/"%C /"/C43;<$ /4%-$4?.$C/[4@@=$%C[4r4> $;5"/" ?&%%$///&/ r+$ /"C/ ">C[! J>.>-r8? +Z# %C/Z #C$///!".'3<' .((6!" #'" %#'" =#>'"6#><'"C# <'"E#<'" )#>N><'"

PAGE 149

= n r 6C".><' r@# r/"%)C3! /"43$4?.$% 4.$;5"/ %//-E//" &/"(! /"%4.r$"%4$C4$C/4" 44"? -cccc!" ? cccc !"?$-$ cccc !"?$-$ $.r cccc !"?$$-$$.r cccc !"

PAGE 150

=% n r r 6E".><' rB# r/"%)C3! /"43$4?.$% 4.$;5"/ %//-E//" &/"(! /"%4.r$"%4$C4$C/4" 44"? -cccc!" ? cccc !"?$-$ cccc !"?$-$ $.r cccc !"?$$-$$.r cccc !"

PAGE 151

== rF"4B r!A "< &//" $ 0$-5/L<" & $ rnr $0 r0 @
PAGE 152

=6 CI-8<8<8<8<8<<<<<88888<8<8<<8< 88 <8<<<8<8<<8<<88<<<<88<888<<<<<<8 <8<<8<8<<88<888<88888<<8<8< < <8<8888<88<8<<888<888<<8<<8<888<8 8 <888<<888<8<<<<<<<8<<8<8<<8<88 8 <<<<<<<8<<<<<<<8888<<<<8 -=I23430 CI=I0 r0# $ %$n$n%" n%.@ =ICI$$ %n #CI-<8<<88<888<8-=I %#CI-8<888<<888<-=In#CI-8<<<<88<<<8<-=In%#CI-<8<<<8<<88<8-=I.0 8nCI=I$ ">.@

PAGE 153

=C 0CI =In n.@"0 !0 12 CI=I 0 #CI-8888888<888-=I %#CI-<<88<8<<8888<-=In#CI-8<<<<88<8<<88-=In%#CI-<<<8<<<<<<<-=I.G@?#CI-8<<8888888<-=I?n?F#CI-8<<<<<<<<888<<<<<8-=Ir+ #CI-<<88<88<<<-=Ir+% #CI-8<<<8<8<88-=Ir+n#CI-888<<8<<88-=Ir+%n#CI-8<88<<888-=I 2343$0 r
PAGE 154

=E "0 ?&r> #33"&"33" !" @>0CI-=I

PAGE 155

=) r>0!F.F?'?8 '>.>'> ''H'?F .@'''r> 8>.FA9>8?@ ;.>8F8AHr. ?HF .rAA?.H;88 ''. ';rr'.+8.F. 8@.r8?HAA>nFn<8n>498 Hr @r>>8?8?'89 Hr 84'?F9 9'A..; '?8.n 9@49488. 89FHnnF F@.A?'nAr >;..n8 F;A 'Fr'.''??F8'H@9Fn8 n8?;??8 8FH.A>@'nn48?H.F'?4@.8 A?A'?> >r $#J0 0 $ 0 nr 6)!"0 "'$ r $ <'

PAGE 156

=5 <4' ;B4' r4' @4' B' 8' 4-.' <-.' >N.' ><.' >N>4<'C/.! <<<' <4<' r .<' ,r .<' r' 6)"rF <'B FA#J0 ">0 @
PAGE 157

=( :nrrA"r< A $ " "G 0 $0$ A$ $ "n#. & $ &$ 5"5 C/" >.>-r8?$ 6=")." $ 0 "n# $"

PAGE 158

6/ &5/.$ >.>-r8?$ / '<' "&' 5/.>,8$(55*+-+" $%//E!"0 0 $ n 8,4$ ()(!";$J $ n r $ 6/.B"$ %//6!" $ $ $ $-$ >"$%//%*n"$(E(! "< & !! ,>$(5C*+"$((/!" "n# $ 0"

PAGE 159

6 /"<4+> <' r'# !r + rrr <' nrn 6=. B"$%//6 >' nn 6/. "$(() ;' $n(n 6/. n"$()) ><' "n#n 5). % +-+"$%//E >N?' nn 5. % +-+"$%//E ><' nrn 5/. % >,8$(55 <' nrn 5/. >"$%//% >' nrn C/. 6 n"$(E( 4<' nn 5%. % 8,4$()( ><' %nrn 5C. % 8"$()( >N><' nrrn C/. ,>$(5C 4<' nrn =%/. 5 +"$((/

PAGE 160

6% !" "n# C/K /6EL'$$ " "n# !$ n nrr +-+"$%//E*n,< $(55!" r; .r!r! "; $)"C-("C$; $E"C-)"/4 $()=*+$()=!" &; 5"/ ;E"/"

PAGE 161

6= ; ; ; .rr!";$; &" 4 .r;: 4.r ;:4r"; & 0 ; r!"; r4.r ;:+$()=!" A! $ & $ "n# E"/ %!"4 >$())*8, 4$()(*+-+"$%//E!";$ % <' E"(<'-446"5<'-

PAGE 162

66 BB8,4$()(!" 44 A" $ $ <' >.>!" & 0" <'-44A)! AE")! AC"5! <'-BBA6!+ -+"$%//E!" $ n $$ n r $$ A<'-BB$ '' & 0 $$0 +-+"$%/ /E!" 0$ $ $$J

PAGE 163

6C %5!" !' +-+"$%//E!"A 0 <' ? "$(E)*8,4$ ()(!'<' >, 8$(55!"4$ <' & '<'O"%//E!" '-<'<''!&C/K'<' %-' &! $$0$ $ "'<' '<' "< NF%C/!"<

PAGE 164

6E <$(56!"& $$ & (K %%% <"$()%!" 8 ,>$((5*8"$ %//=!"(K /-CKB "$%//6* ,8$((E!"A %%%%/5 <.' "n# $ $&

PAGE 165

6) rAA"A A 0"0 " 0 >$ 0 $$ &$ '' '<' 0" A %= 0 E!"@&'$ n $-

PAGE 166

65 5/.>"$%//%!" '$' "@ nr C/.n$(E(!"r$ <'-44 <'-BB";$$ )nr $$ *n $ $ n n"$(5(*,>$(5C!" $ nr $ "A $ $ "A' +$()*+$()C!"A% ///$? $ 0" 12' $ "n# $ (!$ 0

PAGE 167

6( "n #n "A()/$F" 0 $ 0 <'"<'-44 44 -BB 8,4$()(!" $ nr "0 $ "n# F"$()/*B"$%//6! ' '<'". -' <' '"

PAGE 168

C/ -' " & "

PAGE 169

C "
PAGE 170

C% rAAA"' $ &$ "r &P?>Q !& P?>>%Q! '"$(C6!" ''&" A$ ''"// 4 /"C4'!/"==4'! 4.r" $ '' &/"=E4 /"/=64'! /"/)4'!4r;"$%//)!" -B" "n # 0$ +$()=!" & 4,D$(EC*4,<$(EE* F"$(EC*GI> "$(E(!"

PAGE 171

C= 0 & &-?-4rr -?-4.r!>$()C!" $ $%" "@ /"/=6]"% 4#r$/"/C]/"C4#.r!J 4, D$(EC*B,8$%//6*;"$% //)!";$ "< 4,D$(EC*;"$%///*< "$%///!"G $ ''"//4 >,8$(55* N,>$%//6*>())!"& % 'B ,8$%//6*;"$ %//)!" '3' 1 2>$()C!"

PAGE 172

C6 &"// /"C"C 4.r4r;"$%//) !" $ & %$ $ +$()=* <,+$()C*4,D$()=*B ,8$%//6$ "$%//)!"A; "$%///! ";$ "n# &;"$ %//)!"

PAGE 173

CC %"<4'$.'$ .<'. < /"=E /"=) /"/=6 /"/) ;"$%//) n C"E E" "% /"65 4,D$ (EC & r r r !# !# < /"6C /"6( /") /"6 B,8$ %//6 >< "// "// /"C/ /"== ;"$%//) n 5"E %"( /") /"/C 4,D$ (EC

PAGE 174

CE > & ".-$ -$-$-$$ $ 65!" # &$ -$ -CK !+-+"$%//E!" 4$()=!" >$$ $ B,8$%//6*+-+"$%//E* n$())!" -65!"-.' -"$%//*B $()%*4$()=*B, 8$%//6!".& &" .$ -&!'

PAGE 175

C) n n n n G< r r r ;< 8 65"<>> >.#

PAGE 176

C5 0$ & $$4-+, +$()!" -& $$ $B,<$ ()6!"&&' .r! =(!"A -& .r +$()=!"G '$ C"CK" &" F)EK55K"@ 4-+,+$()$4$()=*B ,<$()6!"A

PAGE 177

C( =($6/$6 $ $ "n# $ nr $ '& J&B,8$%//6!"B8 $ "n# $-& =/K CK" & -'Z5"/4! -'ZC"C4!" '">$()C!"< & '$ "n# $$ nr ".0 %//-E //!"r

PAGE 178

E/ =// %5/-=//" M%5/ ,+$()C!"?& >& & ". ' 6%6C!" $$ %)/-=//" 'J <'8, 8-n$(5)!' ,+$()C! $ <'4, +$())!'n "$((E!" %5/-%(/ 6E!" $$

PAGE 179

E =/KF' B,8$%//6!" $$$4.r! $%E/ >" B8 %//6! -& -&

PAGE 180

E% rAF"?n CI=I0 $ rnr $"0 0 #n@ $ n@ .@$$r0 @E=$8E6$FEC$E5$8%%C$<%)$ 8=6 0 6(! r %6$%E$%%($ %5/$=/( $ 0 6(!"

PAGE 181

E= A(()>"0 0 8>! "0 "C 6( nr C E%E=$ N,>$%//6!" n r 6( @? ?.-& ?"$ %///*<"$%//!">$0 6(!<@ & & O"$ %///*"$%//6!"

PAGE 182

E6 !F.F?'?8 '>.>'> ''H'?F .@ '''r> 8>.FA9>8?@;.---->8F8AHr. ?H F .rAA?.H;88 ''.';rr '.+8.F.8@. r8?HAA>nFn<8n>498Hr @r< ?9H'?4?.'F>> >8?8?'89 Hr84'?F99' A..; '?8.n 9@4 9488.89FHnnFF@.A?'nAr >;..n8 F;A'Fr'.''?? F8'H@9Fn8n 8?;??88F H.A>@'nn48?H.F'?4@.8A ?A' ?>>r 6("@>0' < <><> # 0 44"8 ;#< "B; #< "H;#A C 8>8>!"N# @@-! <8-F!&"

PAGE 183

EC 5! ". $ ME/K0 >"$(((!" 0$ $0 "A%//=$<&" <'! "n85$C=$8C)$ E$>6E$6)$C/$ C$%/( 6)$C$%/( < &"$%//=!"4 $ nr $ $ ;"$%///!"A%//C J "E% (= r '! ">J "%//C! "' $ nr $

PAGE 184

EE B,8$%//6!"n 0 E%(= nr ;"$ %//)!"> $ n $E% (= & "$%//)!" 0"A $ 4,+$(E )*+$()*" ()C!"B ( $$ ;$%//)!"4 ". ' <'"A r' 0' r"$%///!"A $'<'

PAGE 185

E) $ '<' 6)$ 0 $ & 0"'0 nr ". $$ "r "%///! $ nr $ 0 A$$ r $ <'

PAGE 186

E5 rF"< r r" r r 0$ $ " $ r r $$ "n# $ $ $$ "n# M5/."A$ n r B"%//6! M6/."+ $0 ".$ $ "0 0 $$ g

PAGE 187

E( $0& B,r$()5*4,D $(EC!"4 0 "<&"%//=! 0 "4$"%/ /)! <' 0 "< 0 &"4 < "$((C*B"$%//*8,>$%/ /)!" n r $ B,>$(E5!" "n# r ". $$ -"

PAGE 188

)/ 4 $0 0$$ $ B"$%//6*B ,8$%//6!" 4 ,D$(EC!"< "' 0 <'$ "n# nr &" $ ;" $%///!$$ "

PAGE 189

) 4-+ ,+$()$4$()=* B,<$()6!" B,8%//6! $$ &J -& -& nr "n# "@ $ B,8%//6! '"A $$ I"B & $ O"$ %//=!"$ $ & &

PAGE 190

)% 0 >"$(((* >"$%///!". 0 $ nr $0 $ & $ J0 C 0O "$%///*N,>$%//6!"G$ 0 $ ?"$%///* O"$%///*<"$ %//!" "n# +0 $ ">$ nr CI=I0

PAGE 191

)= Ar $8"."$r$<""$n$4">"$r $<"$<$""$A$4">"$ 8B$@"4"$$;"@", $4" 4"%//!" nr & 0)!n 6(5$%%-%C" $?"G"$4$B";"$,+$."<"( )C!"? #r r r n r !rrn1 6C$C=C-C6=" ?"G",+$."<"()C!"? !rrn1 6($=5)-=(C" $"4"$4$;"$F$<"$,4$G" %//)!". $ 'rnn!rrnn!rr 6C5$C5-EE" $"$<$>"$?$+"n"$,<$4 ">"%//6!" rnr =$C)C-C5C" B$8"+",>$+""(E5!"A 8 1nn)rn)n E$6%E-6="

PAGE 192

)6 B$>"r"$,8$r"D"(5!" # r %$665-6C%" B$>"r"$,<$<""(5C!" 'n2r3nrn!rr C6$5=-5E%" B$4"$4$F"$;$B"$>$+"n"$> $4"$r$>"$+$ 8"$F$r"$F$n"$,.$4"%//!" 4 A $r $-" 1nn n" E)$C6)/-C6)E" B$>""()%!"' 'rnrn!rrnn!rrn 6( %66-%C" B$>"",'$@"G"(E5!"< n!rrn )$%%=-%=C" B$4"4"()E!" 0 0 -" 'rn n!rrn )%$%65-%C6" B$"?"$ $n"4"$,8$>";"%// 6!"r rnr !" 'rnn"n!rrnnr CE$C-E/" B$.";",<$4"()6!"A $$ 1nn!rrnrn %6($C)==-C)=("

PAGE 193

)C <$D">"$@$+"n"$+$r" "$4 $4"D"$'$8""$, B$r"<"%//!"4 # !rr 6/$=/CE-=/E"<$+"n",+$."<"()C!"' $n !" !rrrnn!rrn' 6/$((-6" <$+"n"$,+$."<"(56!" "rn1nn!rr E$)-)6" <$H"$H$D""$,4$;"4"()%!". !rr $6%/-6=" <$";"$+$<"B"$B$r"<"$4$ 4"D"$,'$8""%///!" & ?" 1nnrnr ($C(-EE" <$r" "$'$8""$,<$+"+"(5! "N; !rrn %/$ %/6-%/"<&$D"4"$.$<"$<$<"$D$4"$DJ $"."$B$4"$>$4"D"$ B$<"$,?$r"A"%//=!"8 #0 !rr 6%$5E=-5)"

PAGE 194

)E <$"?"(56!"A rnnn$nr "@H# +";" <"<$D" "$r$<"$;$n"$>-r $>"$GJ$A"$n$"$4 ?$4",GJ$D""((C!""((6!"r# "A n nr 'n nr F"%=$ n "%6%!"@H#G&r" ?$4"$>$4"$+$"$,>$N "%///!" 1nn!rrnr %)C$%)/(6-%)/((" ?$r""$>$'"$'$?"$4$ @"$,B$<""%//!"< #-(C !rrr n!rrn' C6E$%(-%(5" ?$n"+"%///!" # rn!rrnnr %E$ -)"?$+"n"%//!"? 'n2r3nnr E=$%5(-=%C"

PAGE 195

)) ?$+"n"$,'$>""((5!" !rrn!r (C$%E6-%)%"?$";"$4$D" ",n$;"(CE !"" n!rrn1n %= =C5-=E%" ?$;"4"$.$."4"$,'$@"G" (E)!" "A 1nn!rrnr %6%$%/6-%/(" $D"n"(5(!" -@. 6/C" 'rn!rrn 5%$=((-6/6" $D"n"$,F$<">"(C)!"8 -" !rrrnn!rrn' %C$ C)C-C)5" $B",+$."<"()=!"r G&" !rrn1 =C$%EC-%)E" $4"$'$4"$$r"$,+ $"((5!"> !rrn1 )C$/E-/%=" $n"4"$>$.">"$,8$>";" (()!"r$$ 8 n n !" 'rnn!rrnn!rr =6C$)=-)5"

PAGE 196

)5 -+$'"$>$"$+$"$,' $+"((E!"> 4 =5$=6-=6C" J$@"$$'"$,>$"%//C!" E%(= 0)! C)($E55-E(%" 8$D"<"$,>$<"%//)!"< 'n2r3nrn) C%$6=(-6E=" 8$'""$8$>";",>$."."(5E !"r %rnr rn!rrnnrn 5= E=-E%/" n 8$D"n"(5!" !rrn n2r $C(C-E/" 8$."?"$4$D" "$,?$";" (C)!" !rrn1n EC C=-E%" 8$4"$ -8$?"4"$+$"$? $;"4"$, $n" ((6!" # %%=$%/E$%E60 rnr =$/C5-/E5" 8$>";"(5=!"A # 1nn4rn 6 )%(-)=E"

PAGE 197

)( 8$>";",4$?"()(!"r "rn1n !rrn / =E)-=5" 8$>";",8-n$";"(5)!"A #' 1nn)n"r#rr $=/-=/(" 8$>";"$,>$.">"((5!"> # !rrrn !rrn' =5)$66)-6C=" 8$>"$8$O"$8$9"$<$B"$,+$S"%/ /=!"?&$$ rnr r )(rnnrrr %($%=/-%=6" ;$B"<"%//)!"?& # n !?"5!"A 50nrnr "N> .<" ;$B"<"$+-+$B"$,8$>";"% //)!"' # rn!rrnnr #/"/E3J""%//)"/="/E $-5" ;$r"",>$8"@"()=!"A rnn!rrn'r "=C5!"r$r#+"B"> <"

PAGE 198

5/ ;$"$n$."$,+$"%/ //!"+ g? !rrrnn!rrn n' 65/$=EC-=)=" ;$4"D"$n$n"<"$,4$">" ((C!".r # =r-@4n 4$2n!rr 5$)%-)5" D$;"$;$;"+"$,'$4"(E6!" ; !rrnn!rrn2n n E$CE-C%" '$r"$'$8"$r$"$,+$" (()!" 'n,n!r C$%)C-=%" '$>""$@$"$,$;""(C6!" r!"A 1n !rrnr %/($(-%/" '$n",4$n"((5!" & $ 'rnr %)4)n % =6=-=6(" '$>"$$'"$,@$4"%//6!"4?8=#A 0 !rrnr !rrr C$C/-E="

PAGE 199

5 '$A"$4$B";"$,+$."<" ()/!"r CI-" !rrn1 )$C=-C%=" $>"."$+$r" "$B$r"<"$4 $4"D"$'$8""$,$'"@" %//%!"%" nrr & .r-4%:@G=--&" !rr 6$=5E-=5E)"$D"n",F$n""()(!"?; 4%:'0 1nn!rrnr %C6$EC%5-EC=)" $N"'"$()/!"< 6" 4 %%)$E5/-E5C" $+">"$r$+"$+$4"$,B$.";" ()5!"> ;" n1nn!rrnr %C=$E%%E-E%=%" 4$F"+$+""())!"r 'rnn!rrnn!rr 56$/=-/" 4$""$@$?""$,'$>""(E %!"> #AAA"A 1nn!rrn nr %=)$C=C-C65"

PAGE 200

5% 4$"8"%//=!"-"A )nrr6n'nn n ""%!"@D#D+>" 4$4"D",'$8""%//C!"n rrn2r3nrn!rrnn$n!r 6/$-%/" 4$<";",'$D";"(5C!" # !rr %6$))%-))5" 4$n""$>$;""$,'$4"D" (56!" 12" 'rn1nnrnnn nnr %6E$=EC-=))" 4-+$?"D"$,+$."<"()!"A CI-&# !rrn1 %%$ )%)-)6/"4$D" "()=!" "A n n) F"5$"6C)-65E!"@H#r" 4$D" "$8$."?"$,?$"4" (C)!"r !rrn1n EC 6=-C=" 4$D" "$GI>$D"$,G$"8" (E!"' !rrrnn!rrn' C%$5%-(E"

PAGE 201

5= 4$D" "$,D$?"(EC!" !rrn1 ()$ =)-C%"4$D" "$,<$+"+"(EE!"A & # 1nn!rrnrn %6$E)=-E5=" 4$D" "$,+$"(E)!"A& r#" )n1nn!rr =$ 6C-C%"4$>"4"$+$"$B$N"$,r $D"<"((E!">0 $nn n!rr ==$%6C-%E%" @$"$@$"$,4$4" "(E!"' 0 -" 1n !rrnr %=E$=%/%-=%/(" @$+"n"$+$r" "$4$4"D"$'$ 8""$,B$r"<"%//6!" AE(=%C !rr 6=$=)EE-=))6" Gh>$+"D"$F$n"$,B$>"(E(! "4 )n1 n!rrn 5 CE%-C)/"

PAGE 202

56 r$<""$$8"."$r$4"<"$A $"$<$4""$$ ;"@", $4"4"%///!" 1nn!rrnrn %)C 6(C-C/" r$<""$$8"."$A$>"$B$ ""$$;"@", $4"4" %//=!"> nr 1nn)rn$r#rn C/ =%-=6" r$""$'$n"$n$ ",$@" F"(E6!">hr# !rrrnn!rrn'n 5( %CC-%EC" n$<"$B$ ",B$n"((E!"< n)n1n !rrn %66 =6=-=C" n$"$,<$n"(55!"r -" )n1nn!rr =$EE)-E)6" n n$"$>$B"4"$,<$n"(5(!"r # ?&" )n1nn!rr 5E$(C-%/=" n$8";"$,<$4"()%!"> "? & &" 1nn n!rrnr %6)$=/)=-=/5"

PAGE 203

5C n$H"(E6!"B rn n!rrnnr %$=6)-=E)" n$H"()6!"r !r E$6(-CE" n$H"$'$<",$@"F"(E(!"> hr#$ E/$///" !rrrnn !rr 'n ) .n =C)-=C(" n$H"$B$H"$,$@"()(!"?& 0)!n E=$ )6-)5"n$H",8$H"(5/!"< $ 'rrn7r 'n !r rrr *r C$C)C-5/" n$>"B"(E)!" n n1nn#nnrrn$rr E($E(E-)/C" n$8""$.$.""$,n$8"+ "())!"$ $n(n89 1nn!rrnrn %C% =E)(-=E5=" n$r"n"$.$r"$'$8""$,4 $">"(5!"@ !rr %/$ECC-EE6"

PAGE 204

5E n$<"$'$n"$r$""$,$@" (E5!". r #" !rrrnn!rrn' E)$=%E-=%5" n$<"$r$""$'$n"$ $" $,$@"()/!" > r# !rrrnn n!rrn' %/E$=E(-=)(" >$N"$,>$N"%//6!"A $#rn)rr E$ %%/-%%5">$4"$,<$+"+"()=!"A 1nn!rrnr %65$565-56%=" >$D"."$,8$>";"(55!"r rnr rn!rrn r 5($)/-)/)" >$A";"()C!"B 0 "A )n+rr "%)=-%5=!"@H#+-A" >$?",4$D" "(E(!"' n n1nn!rrnrn %66 6%%6-6%=6"

PAGE 205

5) >$@"$<$+"$$"$F $r"$,B$<" %//E!" rnr J J )rnn nr 6$5)C-55/" >$'"$G$ "$;$"$;$ "$F$"$B$4"$ n$+"$B$"$D$<"$. $D"$$r"$J$n"$ D$r"$D$+"$r$."$,+$B"(( 5!"< $n !rr 56$5=-(6" >$'"B"())!"r< 0$ rnrr !rrnn !rrn )( C5-C%E" >$", $"((6!"? #r 1nn n$n!r $=C=-=C)" >$"$'$H"$, $"(()!"? A$.@0'nr !rrn1 =%5$=/-=/E"

PAGE 206

55 >$"$'$H"$N$H"$@$H"$ >$"$'$4"$H$ H"$, $"((5!"8 0 rnrr !rrrnn!rrn' =55$%C=-%C(" >$"$'$4"$A0$@"$'$;" H$H"$ $"$, ?$+"n"(((!" # !rrn1 =6/$ E)-E)C">$"$A$@"$;$"$4$n"$ >$@"$H$'"$, $"%///!"8 n 'rn nr #A.@-0" rn n!rr nr %)$C/C-C%" >$"$H$H"$,N$4"%///!" rnr # 0 !rrn1 =C$C)(-C5C">$"$>$@"$$"$N$H"$ 4$"$;$4"$ H$'"$N$'", $"%//%!"nr #rnr #?& .E%8" n"rn1nn n!rrnn !r =6$%%-%%("

PAGE 207

5( $'"$>$A"$A$'"$H$> "$,>$"%//)!" rrn G!"4$ "n!rrnn n$n!r =)$==5-=6C" $D"."$8$"D"$r$ "$D $ "$,;$."8"(()!" $B"4"(5C!"4 # 6$=%C-==6" $n"4"$B$<"D"$,>$B"4"(5 )!"<" !rrn1 C%$ )C-5E"N$'"$,>$"%//6!"n 8> r ,r rn1 %=$C=-E6" N$'"$ J$@"$$H"$4$'"$ $'"$?$+"n"$,>$ "%//E!"? rn n!rrnnr $%/(-%5" N$4""$4$ "$,4$D" "(EE!" # "r 1nn!rrn nr $C6%5-C6=C"

PAGE 208

(/ F-r$D"$>$8"<"$,$."()% !".$ n!rrn1 %E$=%C-=6C" F$@"(E5!"A nnn#rrnr "((-%%(!" @H#8BF$@"$,n$H"(E(!"8 "A rn: F"C65$"E=-%/=!"@H#r" F$4"$@$'"$+$'"$4$4"$ ,'$D";"()(!"< "@ CI-" !rr 5$C/C/-C/CC" F$B"$n$H"$,$@"F"()/!"< 000 rn!rrnnr =%$ 6/-6/6" F$n"$+$."<"$,B$?"(EC!" CI-# !rrn1 (6$C=E-C66" +$"$+$4"$B$."$@$'"$ ,?$;"4"((%!" A$$ # 12 !rrn1 %5$%-6/"

PAGE 209

( +$"$.$4"$>$N"$?$4" $;$"$GI8$?"$ n$"$,B$."((5!"> <'!# $$ n!r0 5$%%(-%=6" +$r" "$4$4"D"$'$4"4"$$8"$ ,'$8""%//!" '<%5% !rr 6/$E(5-)/C" +$r" "$'$8""$,4$4"D"%//E! ";?"-& "5!$!nr C5$6%-6%5" +$."<"()!"? "A !rrn)rnnn n*rrnnr F"=(5$"C/-)=!"@H#8,B +$."<"()=!"";"%//E!"A #> !rrrnn!rrn' )E/$=E6-=)" +$4"$>$D"$D$r"$?$;" 4"$,+$"((/!" 4# $ 1nn!rrn r %EC$C(//-C(/5"

PAGE 210

(% +$4"$+$"$,'$D"((=!"> @--=$ =I$C----" !rrn1 %($6E=-6)%" +$4"$,'-.$n"%///!"< rn2r3 5/$/)-%=" +$4"$,>$"%//%!"; # g 4r %$%6=-%E/" H$4">"$<$>""$r$r"'"$? $+"n"$,<$4">"%//=!" A" rnr %$/=-" H$H"H"$'$>";"$r$D"+"$$A"H"$n $;"A",;$<">"%//!" <8 &" 'n'n'n"n 5E CC-CCE" O$D"$8$9"$O$"$$"$,+$S" %//E!" rnr !rr )$(5=-(55" O$8"$>$"$B$?"$r $8"$?$+"n"$, <$4">"((5!" # rnnn4rn'nn nr (C$566(-56C6"

PAGE 211

(= O$"$8$D"<"$>$>"4"$+$. "+"$+$4"8",>$<" %//=!"B 1nn)nn)n (E =E%-=EE"

PAGE 212

nrn nn nnn n! # #$%nn n&rn' 'n# n#(#!# "n nn!"n$ nn n#"#n")