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Synthesis and characterization of interfaces between naturally derived and synthetic nanostructures for biomedical appli...

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Material Information

Title:
Synthesis and characterization of interfaces between naturally derived and synthetic nanostructures for biomedical applications
Physical Description:
Book
Language:
English
Creator:
Zekri, Souheil
Publisher:
University of South Florida
Place of Publication:
Tampa, Fla.
Publication Date:

Subjects

Subjects / Keywords:
Biosensor
Porous silicon
Orthopaedics
Tissue engineering
Collagen
Carbon nanotube
Dissertations, Academic -- Mechanical Engineering -- Doctoral -- USF   ( lcsh )
Genre:
bibliography   ( marcgt )
theses   ( marcgt )
non-fiction   ( marcgt )

Notes

Abstract:
ABSTRACT: The use of nanotechnology to develop methods for fabrication and characterization of organized hybrid nanostructures that include integrated polymeric, biological and inorganic compounds has increased exponentially during the last decade. Such bio-nano-composite materials could be used in solving current biomedical problems spanning from nanomedicine to tissue engineering and biosensing. In this dissertation, a systematic study has been carried out on the synthesis, characterization, of two interfaces between naturally derived and synthetic nanostructures. Carbon nanotubes and porous silicon represent the synthetic nanostructures that were developed for the purpose of interfacing with the naturally derived bovine type I collagen and respiratory syncytial virus DNA respectively. Firstly, the synthesis of collagen-carbon nanotubes by two different techniques: fibrillogenesis through slow wet fiber drawing (gelation process) and electrospinning has been highlighted. Characterization of the novel nanocomposite was conducted using electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, nanoindentation, and Raman spectroscopy. The collagen-carbon nanotube gelation process was found to have superior nanoscale surface mechanical properties that were more conducive to higher osteoblast specific protein expression such as osteocalcin. Applications of the developed nanofibers are detailed in the fields of orthopaedics and tissue engineering. Secondly, an overview of porous silicon synthesized by hydrofluoric acid is presented. A parametric study was performed to determine the optimal pore size was carried out. The use of porous silicon as a biosensor to detect RSV virus by DNA hybridization was then provided and the importance of the interface chemistry was highlighted.
Thesis:
Dissertation (Ph.D.)--University of South Florida, 2007.
Bibliography:
Includes bibliographical references.
System Details:
System requirements: World Wide Web browser and PDF reader.
System Details:
Mode of access: World Wide Web.
Statement of Responsibility:
by Souheil Zekri.
General Note:
Title from PDF of title page.
General Note:
Document formatted into pages; contains 142 pages.
General Note:
Includes vita.

Record Information

Source Institution:
University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 001921018
oclc - 190844556
usfldc doi - E14-SFE0002132
usfldc handle - e14.2132
System ID:
SFS0026450:00001


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Full Text

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Synthesis and Characterization of Interfaces Between Naturally Derived and Synthetic Nanostructures for Biomedical Applications by Souheil Zekri A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy Department of Mechanical Engineering College of Engineering University of South Florida Major Professor: Ashok Kumar, Ph.D. Rajiv Dubey, Ph.D. Muhammad M. Rahman, Ph.D. Thomas J. Koob, Ph.D. Date of Approval: May 25, 2007 Keywords: biosensor, porous silicon, orthopaedic s, tissue engineering, co llagen, carbon nanotube Copyright 2007, Souheil Zekri

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DEDICATION This dissertation is dedicated to my pa rent, brother, sister, wife and kids.

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ACKNOWLEDGEMENTS I would like to extend my gratitude and thanks to my research advisor, Dr. Ashok Kumar for his continuous support and guidance, which ha s helped and guided me thoughout my doctoral work. I would also like to thank my comm ittee members: Dr. Rajiv Dubey (USF) and Dr. Muhammad M. Rahman (USF). Your input a nd guidance during my research has been invaluable and grea tly appreciated. My specia l thanks go to Dr. Thomas J. Koob for his immense support and guidance throughout a large portion of my research. He has helped me understand the value of interdisciplinary research. Speci al acknowledge go to Douglas Pringle and Daniel Hernandez for their contributions to th e collagen based nanocomposites project. I am grateful to the GK-12 Fellowship for providing with the financial and professional support. My special thanks to Dr. Geoffrey O kogbaa, Dr. Tapas Das, Dr Griselle Centeno and Dr. Martin-Vega for their assistan ce and guidance in the fellowshi p. I would like to recognize Dr. Kumar’s financial assist ance through NSF GK-12 (Grant # 0139348), NSF CAREER (Grant # 9983535) and NSF NIRT grants (Grant # 0404137) I would like to acknowledge the great people at the ME department: Su e Britten and Shirley Trevor for their administrative he lp. I would also like to thank my co-workers and friends from the Advanced Materials lab for all their support. Finally, I would like to thank my friends and family, especially my wife Tara and kids Abdul-Hakim and Abdul-Malik for suppor ting me throughout this endeavor.

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,2 7!'-.AI#<&A#$:A#!//A-( %-A%!8A%!IA #''$!#!$&$43$#-$!"-33/ '%(F62?@,5,, 7,8&:5A$&-A(-:IA:$/$!)A)//(A &$""-! %#!' n-#=! :%#!4$":&/.#//'#-$!#!$& r!'-!/$#' !>>>F76?212,@0,6501> 71+# !-A$&-A/'4#!8A!!5!'&'"-# 4!#$!$" 4&/.#//#-$!!#!$&!#3$/(4-4#-C33 /'%(7F6?@775> 72$&-A$C:A+# !-n&=/! #!'$//# 3$"4'''#-$! #!$&%(#/.-7F7?7@0,7 70%#!4& %#-#:An#IA)8A$%+A A(&%(#/#!' %4#/%#-#-$"4&/.#//'#-$!!#!$ &"&!$!#/;'.% #4!$/#!#!'!"/&!$!%3-$3-$" !#&-#/-&-$43$ $43$!#!'%!$/$ (>>6F06?@7,5 7 76=$/#A:In-$!=$.=A)n-#'/(A $%4&!'A$/-A) 4%A4#//(#53%###/(-$.%$" /5+#//'#-$! #!$&"-$4#-$!:$!$C'%4%( F,, 77#A!!$n""$"%4#/"&!$! #/;#$!$!%4%#!#/ 3-$3-$"#-$!!#!$&%4#/%( -7F2?1@6,567 7%#'/-A#!!#-A#(#!:$#'-#! "-!#-$!!#!$&3$C( $43$33/'%(-7F6,?0@,71 5,711 >O#!A=(A!'-.A#!//$#' -#!"-#!''"$-4#$! 4%#!4!#-$!!#!$&53$/((-!$43$ 33/'%(>>>F60?>@707576> &;=$A#! A#/=$A-&=5I#.$-=#A -$;;%($/$ #/ $"#-$!!#!$&#-%(#$/= \&//-!% >>F?@2521 &!;=-A$/;:Ar#!$%!$/$ (!:'!4$! "-$4 %!%$%''%4#>>F202>520 ,#!%#4/$!<&#!&4.-#--#("#-# $!(/-$%4#/#!' %4#/'$/&$!$".#"-33/'%( ->F26?>@>105>17 1$!=/A%&/;I+/-$%4#/3 $"$-$&/$!$-4#$! I$&-!#/$"$-$&:#-#/>>>F6?@50

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,0 2#&#/A-!#!';IA#!%;&/#$! #!' -$.%$"3$-#!' 3%$$/&4!!!35(33$-$&/$!I$&-!#/ $"33/'%( >>F?@700570 0%4#!!*A$//$-4#$!:%#!4#!'-$3 -$"/-$%4#//( %'-!%!!5(3/$!I$&-!#/$"% /-$%4#/$( >F,6?@02,502 6n-&4%#'A#!%#4A=! :A&"$!I -#4-#!#/($"3$!&/#$!#!'3-$3# #$!!#!$''/$!/ -$%4##,F,7?5 ,@56 7#&#/AI-!]!';A]!%;A:# !$#A &//^5&'#-&&-#/ %#-#-$"35(3$-$&/$!#!'%/#$!$%&/#$!#!' -$.%$"$-I$&-!#/$"$-$&:#-#/>>F ?@26500 &=(/).$!A&%#!=#-%$%A-$.%# !'!&/#$!$"3$-!!5 (33$-$&/$!#!'-/#'3%$$/&4!! :#-#/!#!' !-! n>>,>,57 >>B#$ $-(9%#! /-$%4-($"/ $!#!'C'3-! -F >> >''$.:I!$''$/&$!$"35#!'!5(3 /$!)!&'($"% %4#/4%#!4I$&-!#/$"/-$#!#/(#/% 4-(>F7>?@65 >=-! A:In#/AI$!AI# -$!A-"3#/&'$" %-&&-$"3$-$&/$!"/4"$-4'!35 (3' !-##!'!$!5 !-#/$!I$&-!#/$"%($/'# %(71F6?,2@02,25 022 >,%4#!!*A$/r$-$&/$!"$-4#$! <&#!&4.-""33/' %(-F27?7@7205727 >1! /!%($%4#/3-$3-$"4#/ /4#/3#-/!$/&$! T4-$/-$'T-#$!A%4$-3$!A$43$ 4#/3#-/A#!'% #$45$54#/-#!$!I%(%4,F6? @21265216 >2%/3:$-#-(#!$-&&-'4#-#/I 3$-$!-$ -!%( >>F,?6@ >0nA%##!#$.43-#&-&-" #%4-(#!' !#!$-&&-&-"#!>>F2>>?5,@0750 22 >6n#/#-#A#%!n/$=$3$/(4-!!#! $%!$/$ (.I-(+$-/' !"&/%! F>>,

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Zekri, Souheil.
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Synthesis and characterization of interfaces between naturally derived and synthetic nanostructures for biomedical applications
h [electronic resource] /
by Souheil Zekri.
260
[Tampa, Fla.] :
b University of South Florida,
2007.
3 520
ABSTRACT: The use of nanotechnology to develop methods for fabrication and characterization of organized hybrid nanostructures that include integrated polymeric, biological and inorganic compounds has increased exponentially during the last decade. Such bio-nano-composite materials could be used in solving current biomedical problems spanning from nanomedicine to tissue engineering and biosensing. In this dissertation, a systematic study has been carried out on the synthesis, characterization, of two interfaces between naturally derived and synthetic nanostructures. Carbon nanotubes and porous silicon represent the synthetic nanostructures that were developed for the purpose of interfacing with the naturally derived bovine type I collagen and respiratory syncytial virus DNA respectively. Firstly, the synthesis of collagen-carbon nanotubes by two different techniques: fibrillogenesis through slow wet fiber drawing (gelation process) and electrospinning has been highlighted. Characterization of the novel nanocomposite was conducted using electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, nanoindentation, and Raman spectroscopy. The collagen-carbon nanotube gelation process was found to have superior nanoscale surface mechanical properties that were more conducive to higher osteoblast specific protein expression such as osteocalcin. Applications of the developed nanofibers are detailed in the fields of orthopaedics and tissue engineering. Secondly, an overview of porous silicon synthesized by hydrofluoric acid is presented. A parametric study was performed to determine the optimal pore size was carried out. The use of porous silicon as a biosensor to detect RSV virus by DNA hybridization was then provided and the importance of the interface chemistry was highlighted.
502
Dissertation (Ph.D.)--University of South Florida, 2007.
504
Includes bibliographical references.
516
Text (Electronic dissertation) in PDF format.
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System requirements: World Wide Web browser and PDF reader.
Mode of access: World Wide Web.
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Title from PDF of title page.
Document formatted into pages; contains 142 pages.
Includes vita.
590
Advisor: Ashok Kumar, Ph.D.
653
Biosensor.
Porous silicon.
Orthopaedics.
Tissue engineering.
Collagen.
Carbon nanotube.
690
Dissertations, Academic
z USF
x Mechanical Engineering
Doctoral.
773
t USF Electronic Theses and Dissertations.
4 856
u http://digital.lib.usf.edu/?e14.2132