Citation

Material Information

Title:
Genomic characterization of Severe Acute Respiratory Syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences
Series Title:
Journal of virology
Creator:
Drexler, Jan Felix
Gloza-Rausch, Florian
Glende, Jörg
Corman, Victor Max
Muth, Doreen
Goettsche, Matthias
Seebens, Antje
Niedrig, Matthias
Pfefferle, Susanne
Yordanov, Stoian
Zhelyazkov, Lyubomir
Hermanns, Uwe
Vallo, Peter
Lukashev, Alexander
Müller, Marcel Alexander
Deng, Hongkui
Herrler, Georg
Drosten, Christian
Publisher:
American Society for Microbiology
Publication Date:
Physical Description:
1 online resource

Subjects

Subjects / Keywords:
Bats -- Viruses ( lcsh )
Coronaviruses ( lcsh )
Genre:
serial ( sobekcm )
Location:
Europe

Notes

Abstract:
Bats may host emerging viruses, including coronaviruses (CoV). We conducted an evaluation of CoV in rhinolophid and vespertilionid bat species common in Europe. Rhinolophids carried severe acute respiratory syndrome (SARS)-related CoV at high frequencies and concentrations (26% of animals are positive; up to 2.4 × 108 copies per gram of feces), as well as two Alphacoronavirus clades, one novel and one related to the HKU2 clade. All three clades present in Miniopterus bats in China (HKU7, HKU8, and 1A related) were also present in European Miniopterus bats. An additional novel Alphacoronavirus clade (bat CoV [BtCoV]/BNM98-30) was detected in Nyctalus leisleri. A CoV grouping criterion was developed by comparing amino acid identities across an 816-bp fragment of the RNA-dependent RNA polymerases (RdRp) of all accepted mammalian CoV species (RdRp-based grouping units [RGU]). Criteria for defining separate RGU in mammalian CoV were a >4.8% amino acid distance for alphacoronaviruses and a >6.3% distance for betacoronaviruses. All the above-mentioned novel clades represented independent RGU. Strict associations between CoV RGU and host bat genera were confirmed for six independent RGU represented simultaneously in China and Europe. A SARS-related virus (BtCoV/BM48-31/Bulgaria/2008) from a Rhinolophus blasii (Rhi bla) bat was fully sequenced. It is predicted that proteins 3b and 6 were highly divergent from those proteins in all known SARS-related CoV. Open reading frame 8 (ORF8) was surprisingly absent. Surface expression of spike and staining with sera of SARS survivors suggested low antigenic overlap with SARS CoV. However, the receptor binding domain of SARS CoV showed higher similarity with that of BtCoV/BM48-31/Bulgaria/2008 than with that of any Chinese bat-borne CoV. Critical spike domains 472 and 487 were identical and similar, respectively. This study underlines the importance of assessments of the zoonotic potential of widely distributed bat-borne CoV.
Original Version:
Volume 84, Issue 21

Record Information

Source Institution:
University of South Florida
Holding Location:
University of South Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
K26-05409 ( USFLDC DOI )
k26.5409 ( USFLDC Handle )

USFLDC Membership

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Postcard Information

Format:
Serial