A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence

Citation

Material Information

Title:
A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence
Series Title:
Nature medicine
Creator:
Menachery, Vineet D
Yount Jr., Boyd L.
Debbink, Kari
Agnihothram, Sudhakar
Gralinski, Lisa E.
Plante, Jessica A.
Graham, Rachel L.
Scobey, Trevor
Ge, Xing-Yi
Donaldson, Eric F.
Randell, Scott H.
Lanzavecchia, Antonio
Marasco, Wayne A.
Shi, Zhengli-Li
Baric, Ralph S.
Publisher:
Springer Nature
Publication Date:
Physical Description:
1 online resource

Subjects

Subjects / Keywords:
Coronaviruses ( lcsh )
SARS (Disease) ( lcsh )
Bats -- Viruses ( lcsh )
Genre:
serial ( sobekcm )

Notes

Abstract:
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV underscores the threat of cross-species transmission events leading to outbreaks in humans. Here we examine the disease potential of a SARS-like virus, SHC014-CoV, which is currently circulating in Chinese horseshoe bat populations1. Using the SARS-CoV reverse genetics system2, we generated and characterized a chimeric virus expressing the spike of bat coronavirus SHC014 in a mouse-adapted SARS-CoV backbone. The results indicate that group 2b viruses encoding the SHC014 spike in a wild-type backbone can efficiently use multiple orthologs of the SARS receptor human angiotensin converting enzyme II (ACE2), replicate efficiently in primary human airway cells and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo experiments demonstrate replication of the chimeric virus in mouse lung with notable pathogenesis. Evaluation of available SARS-based immune-therapeutic and prophylactic modalities revealed poor efficacy; both monoclonal antibody and vaccine approaches failed to neutralize and protect from infection with CoVs using the novel spike protein. On the basis of these findings, we synthetically re-derived an infectious full-length SHC014 recombinant virus and demonstrate robust viral replication both in vitro and in vivo. Our work suggests a potential risk of SARS-CoV re-emergence from viruses currently circulating in bat populations.
Original Version:
Volume 21

Record Information

Source Institution:
University of South Florida
Holding Location:
University of South Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
K26-05436 ( USFLDC DOI )
k26.5436 ( USFLDC Handle )

USFLDC Membership

Aggregations:
University of South Florida
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serial

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