Phylogenetic structure of unusual aquatic microbial formations in Nullarbor caves, Australia

Citation

Material Information

Title:
Phylogenetic structure of unusual aquatic microbial formations in Nullarbor caves, Australia
Series Title:
Environmental Microbiology
Creator:
Holmes, Andrew J.
Tujula, Niina A.
Holley, Marita
Contos, Annalisa
James, Julia M.
Rogers, Peter
Grillings, Michael R.
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
Phylogenetic Structure ( local )
Microbial Formations ( local )
Nullarbor Caves, Australia ( local )
Australia ( local )
Electron Microscopy ( local )
DNA Analysis ( local )
Dna ( local )
Genre:
serial ( sobekcm )

Notes

Abstract:
The nature of unusual aquatic microbial formations in flooded passages of cave systems in the Nullarbor region of Australia was investigated using electron microscopy and DNA analysis. The caves are located in a semiarid region but intersect the watertable at depths of approximately 100 m below the surface. Throughout submerged portions of the caves divers have noted the presence of unusual microbial formations. These ‘microbial mantles’ comprise sheets or tongues of mucoid material in which small crystals are embedded. Examination of the biomass revealed it to be primarily composed of densely packed, unbranched filaments, together with spherical‐, rod‐ and spiral‐shaped cells, and microcrystals of calcite in a mucoid matrix. Molecular phylogenetic analysis of the community structure revealed ∼12% of clones showed high similarity to autotrophic nitrite‐oxidizing bacteria (Nitrospira moscoviensis). The remainder of the clones exhibited a high proportion of phylogenetically novel sequence types. Chemical analysis of water samples revealed high levels of sulphate and nitrate together with significant nitrite. The community structure, the presence of nitrite in the water, and the apparent absence of aquatic macrofauna, suggest these microbial structures may represent biochemically novel, chemoautotrophic communities dependent on nitrite oxidation.
Original Version:
Environmental Microbiology, Vol. 3, no. 4 (2001-12-20).

Record Information

Source Institution:
University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.

USFLDC Membership

Aggregations:
University of South Florida
Karst Information Portal

Postcard Information

Format:
serial

printinsert_linkshareget_appmore_horiz

Download Options

close

No images or PDF downloads are available for this resource.


Cite this item close

APA

Cras ut cursus ante, a fringilla nunc. Mauris lorem nunc, cursus sit amet enim ac, vehicula vestibulum mi. Mauris viverra nisl vel enim faucibus porta. Praesent sit amet ornare diam, non finibus nulla.

MLA

Cras efficitur magna et sapien varius, luctus ullamcorper dolor convallis. Orci varius natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Fusce sit amet justo ut erat laoreet congue sed a ante.

CHICAGO

Phasellus ornare in augue eu imperdiet. Donec malesuada sapien ante, at vehicula orci tempor molestie. Proin vitae urna elit. Pellentesque vitae nisi et diam euismod malesuada aliquet non erat.

WIKIPEDIA

Nunc fringilla dolor ut dictum placerat. Proin ac neque rutrum, consectetur ligula id, laoreet ligula. Nulla lorem massa, consectetur vitae consequat in, lobortis at dolor. Nunc sed leo odio.