Analysis of methanotrophic bacteria in Movile Cave by stable isotope probing
- Permanent Link:
- Analysis of methanotrophic bacteria in Movile Cave by stable isotope probing
- Series Title:
- Environmental Microbiology
- Hutchens, Elena
Dumont, Marc G.
McDonald, Ian R.
Murrell, J. Collin
- Publication Date:
- Subjects / Keywords:
- Movile Cave ( local )
Groundwater ( local )
Groundwater Ecosystem ( local )
In Situ ( local )
Chemoautotrophic Production ( local )
Methane ( local )
Microbials ( local )
- serial ( sobekcm )
- Movile Cave is an unusual groundwater ecosystem that is supported by in situ chemoautotrophic production. The cave atmosphere contains 1â€“2% methane (CH4), although much higher concentrations are found in gas bubbles that keep microbial mats afloat on the water surface. As previous analyses of stable carbon isotope ratios have suggested that methane oxidation occurs in this environment, we hypothesized that aerobic methaneâ€oxidizing bacteria (methanotrophs) are active in Movile Cave. To identify the active methanotrophs in the water and mat material from Movile Cave, a microcosm was incubated with a 10%13CH4 headspace in a DNAâ€based stable isotope probing (DNAâ€SIP) experiment. Using improved centrifugation conditions, a 13Câ€labelled DNA fraction was collected and used as a template for polymerase chain reaction amplification. Analysis of genes encoding the smallâ€subunit rRNA and key enzymes in the methane oxidation pathway of methanotrophs identified that strains of Methylomonas, Methylococcus and Methylocystis/Methylosinus had assimilated the 13CH4, and that these methanotrophs contain genes encoding both known types of methane monooxygenase (MMO). Sequences of nonâ€methanotrophic bacteria and an alga provided evidence for turnover of CH4 due to possible crossâ€feeding on 13Câ€labelled metabolites or biomass. Our results suggest that aerobic methanotrophs actively convert CH4 into complex organic compounds in Movile Cave and thus help to sustain a diverse community of microorganisms in this closed ecosystem.
- Original Version:
- Environmental Microbiology, Vol. 6, no. 2 (2003-12-04).
- Source Institution:
- University of South Florida Library
- Holding Location:
- University of South Florida
- Rights Management:
- This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.
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