Diversity of methanotroph communities in a basalt aquifer

Citation

Material Information

Title:
Diversity of methanotroph communities in a basalt aquifer
Series Title:
FEMS Microbiology Ecology
Creator:
Newby, D. T.
Reed, D. W.
Petzke, L. M.
Igoe, A. L..
Delwiche, M. E.
Roberto, F. F.
McKinley, J. P.
Whiticar, M. J.
Colwell, F. S.
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
Methanotrophs ( local )
Sequence Diversity ( local )
Methane Monooxygenase ( local )
Smmo ( local )
Pmmo ( local )
Genre:
serial ( sobekcm )

Notes

Abstract:
Methanotrophic bacteria play an important role in global cycling of carbon and co-metabolism of contaminants. Methanotrophs from pristine regions of the Snake River Plain Aquifer (SRPA; Idaho, USA) were studied in order to gain insight into the native groundwater communities' genetic potential to carry out TCE co-metabolism. Wells were selected that were proximal to a TCE plume believed to be undergoing natural attenuation. Methane concentrations ranged from 1 to >1000 nM. Carbon isotope ratios and diversity data together suggest that the SRPA contains active communities of methanotrophs that oxidize microbially produced methane. Microorganisms removed from groundwater by filtration were used as inocula for enrichments or frozen immediately and DNA was subsequently extracted for molecular characterization. Primers that specifically target methanotroph 16S rRNA genes or genes that code for subunits of soluble or particulate methane monooxygenase, mmoX and pmoA, respectively, were used to characterize the indigenous methanotrophs via PCR, cloning, RFLP analysis, and sequencing. Type I methanotroph clones aligned with Methylomonas, Methylocaldum, and Methylobacter sequences and a distinct 16S rRNA phylogenetic lineage grouped near Methylobacter. The majority of clone sequences in type II methanotroph 16S rRNA, pmoA, and mmoX gene libraries grouped closely with sequences in the Methylocystis genus. A subset of the type II methanotroph clones from the aquifer had sequences that aligned most closely to Methylosinus trichosporium OB3b and Methylocystis spp., known TCE-co-metabolizing methanotrophs.
Original Version:
FEMS Microbiology Ecology, Vol. 48, no. 3 (2004-06-01).

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University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.

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