Characterization of Microsatellites in Pseudogymnoascus destructans for White-nose Syndrome Genetic Analysis

Citation

Material Information

Title:
Characterization of Microsatellites in Pseudogymnoascus destructans for White-nose Syndrome Genetic Analysis
Series Title:
Journal of Wildlife Diseases
Creator:
Drees, Kevin P.
Parise, Katy L.
Rivas, Stephanie M.
Felton, Lindsey L.
Puechmaille, Sebastien J.
Keim, Paul
Foster, Jeffrey T.
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
P. Destructans ( local )
WNS: North America ( local )
Pathogenic Fungus ( local )
DNA Analysis ( local )
Genetic Diversity ( local )
Genre:
serial ( sobekcm )

Notes

Abstract:
Despite only emerging in the past decade, white-nose syndrome has become among the most devastating wildlife diseases known. The pathogenic fungus Pseudogymnoascus destructans infects hibernating bats and typically leads to high rates of mortality at hibernacula during winter in North America. We developed a set of genetic markers to better differentiate P. destructans isolates. We designed and successfully characterized these 23 microsatellite markers of P. destructans for use in disease ecology and epidemiology research. We validated these loci with DNA extracted from a collection of P. destructans isolates from the US and Canada, as well as from Europe (the likely introduction source based on currently available data). Genetic diversity calculated for each locus and for the multilocus panel as a whole indicates sufficient allelic diversity to differentiate among and between samples from both Europe and North America. Indices of genetic diversity indicate a loss of allelic diversity that is consistent with the recent introduction and rapid spread of an emerging pathogen.
Original Version:
Journal of Wildlife Diseases, Vol. 53, no. 4 (2017-10-01).

Record Information

Source Institution:
University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.

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