Modulation of DNA Repair Systems in Blind Cavefish during Evolution in Constant Darkness
- Permanent Link:
- Modulation of DNA Repair Systems in Blind Cavefish during Evolution in Constant Darkness
- Series Title:
- Current Biology
- Zhao, Haiyu
Mauro, Giuseppe Di
Foulkes, Nicholas S.
- Publication Date:
- Subjects / Keywords:
- DNA Repair ( local )
Photoreactivation ( local )
Photolyase ( local )
Cavefish ( local )
Evolution ( local )
D-Box ( local )
Zebrafish ( local )
UV Damage ( local )
- serial ( sobekcm )
- How the environment shapes the function and evolution of DNA repair systems is poorly understood. In a comparative study using zebrafish and the Somalian blind cavefish, Phreatichthys andruzzii, we reveal that during evolution for millions of years in continuous darkness, photoreactivation DNA repair function has been lost in P. andruzzii. We demonstrate that this loss results in part from loss-of-function mutations in pivotal DNA-repair genes. Specifically, C-terminal truncations in P. andruzzii DASH and 6-4 photolyase render these proteins predominantly cytoplasmic, with consequent loss in their functionality. In addition, we reveal a general absence of light-, UV-, and ROS-induced expression of P. andruzzii DNA-repair genes. This results from a loss of function of the D-box enhancer element, which coordinates and enhances DNA repair in response to sunlight. Our results point to P. andruzzii being the only species described, apart from placental mammals, that lacks the highly evolutionary conserved photoreactivation function. We predict that in the DNA repair systems of P. andruzzii, we may be witnessing the first stages in a process that previously occurred in the ancestors of placental mammals during the Mesozoic era.
- Original Version:
- Current Biology, Vol. 28, no. 20 (2018-10-22).
- Source Institution:
- University of South Florida Library
- Holding Location:
- University of South Florida
- Rights Management:
- This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.
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