Phylogenetic Diversity of Bacteria in an Earth-Cave in Guizhou Province, Southwest of China

Citation

Material Information

Title:
Phylogenetic Diversity of Bacteria in an Earth-Cave in Guizhou Province, Southwest of China
Series Title:
The Journal of Microbiology
Creator:
Zhou, JunPei
Zou, Changsong
Gu, YingQi
Mo, MingHe
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
16S Rrna Gene ( local )
Karst ( local )
Cave ( local )
Bacterial Community ( local )
Microbial Ecology ( local )
Genre:
serial ( sobekcm )

Notes

Abstract:
The objective of this study was to analyze the phylogenetic composition of bacterial community in the soil of an earth-cave (Niu Cave) using a culture-independent molecular approach. 16S rRNA genes were amplified directly from soil DNA with universally conserved and Bacteria-specific rRNA gene primers and cloned. The clone library was screened by restriction fragment length polymorphism (RFLP), and representative rRNA gene sequences were determined. A total of 115 bacterial sequence types were found in 190 analyzed clones. Phylogenetic sequence analyses revealed novel 16S rRNA gene sequence types and a high diversity of putative bacterial community. Members of these bacteria included Proteobacteria (42.6%), Acidobacteria (18.6%), Planctomycetes (9.0%), Chloroflexi (Green nonsulfur bacteria, 7.5%), Bacteroidetes (2.1%), Gemmatimonadetes (2.7%), Nitrospirae (8.0%), Actinobacteria (High G+C Gram-positive bacteria, 6.4%) and candidate divisions (including the OP3, GN08, and SBR1093, 3.2%). Thirty-five clones were affiliated with bacteria that were related to nitrogen, sulfur, iron or manganese cycles. The comparison of the present data with the data obtained previously from caves based on 16S rRNA gene analysis revealed similarities in the bacterial community components, especially in the high abundance of Proteobacteria and Acidobacteria. Furthermore, this study provided the novel evidence for presence of Gemmatimonadetes, Nitrosomonadales, Oceanospirillales, and Rubrobacterales in a karstic hypogean environment.
Original Version:
The Journal of Microbiology, Vol. 45 (2007-05-01).

Record Information

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University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.

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