Microbial communities in karst groundwater and their potential use for biomonitoring

Citation

Material Information

Title:
Microbial communities in karst groundwater and their potential use for biomonitoring
Creator:
Pronk, Michiel
Goldscheider, Nico
Zopfi, Jakob
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
Subsurface Microbiology ( local )
Ecology ( local )
Groundwater Monitoring ( local )
Pcr-Dgge ( local )
Cloning/Sequencing ( local )
Switzerland ( local )
Genre:
serial ( sobekcm )

Notes

Abstract:
The structure, diversity and dynamics of microbial communities from a swallow hole draining agricultural land and two connected karst springs (Switzerland) were studied using molecular microbiological methods and related to hydrological and physicochemical parameters. Storm responses and an annual hydrological cycle were monitored to determine the short- and long-term variability, respectively, of bacterial communities. Statistical analysis of bacterial genetic fingerprints (16S rDNA PCR-DGGE) of spring water samples revealed several clusters that corresponded well with different levels of the allochthonous swallow hole contribution. Microbial communities in spring water samples highly affected by the swallow hole showed low similarities among them, reflecting the high temporal variability of the bacterial communities infiltrating at the swallow hole. Conversely, high similarities among samples with low allochthonous contribution provided evidence for a stable autochthonous endokarst microbial community. Three spring samples, representative for low, medium and high swallow hole contribution, were analysed by cloning/sequencing in order to identify the major bacterial groups in the communities. The autochthonous endokarst microbial community was mainly characterized of δ-Proteobacteria, Acidobacteria and Nitrospira species. A high percentage of unknown sequences suggested further that many karst aquifer bacteria are still undiscovered. Finally, the potential use of groundwater biomonitoring using microbial communities is discussed.
Original Version:
Vol. 17, no. 1 (2008-08-08).

Record Information

Source Institution:
University of South Florida Library
Holding Location:
University of South Florida
Rights Management:
This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.

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University of South Florida
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