Field trial of a probiotic bacteria to protect bats from white-nose syndrome

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Field trial of a probiotic bacteria to protect bats from white-nose syndrome

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Field trial of a probiotic bacteria to protect bats from white-nose syndrome
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Scientific Reports
Hoyt, Joseph R.
Langwing, Kate E.
White, Paul
Kaarakka, Heather M.
Redell, Jennifer A. et al
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Conservation Biology ( local )
Ecological Epidemiology ( local )
Fungal Infection ( local )
serial ( sobekcm )


Tools for reducing wildlife disease impacts are needed to conserve biodiversity. White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans, has caused widespread declines in North American bat populations and threatens several species with extinction. Few tools exist for managers to reduce WNS impacts. We tested the efficacy of a probiotic bacterium, Pseudomonas fluorescens, to reduce impacts of WNS in two simultaneous experiments with caged and free-flying Myotis lucifugus bats at a mine in Wisconsin, USA. In the cage experiment there was no difference in survival between control and P. fluorescens-treated bats. However, body mass, not infection intensity, predicted mortality, suggesting that within-cage disturbance influenced the cage experiment. In the free-flying experiment, where bats were able to avoid conspecific disturbance, infection intensity predicted the date of emergence from the mine. In this experiment treatment with P. fluorescens increased apparent overwinter survival five-fold compared to the control group (from 8.4% to 46.2%) by delaying emergence of bats from the site by approximately 32 days. These results suggest that treatment of bats with P. fluorescens may substantially reduce WNS mortality, and, if used in combination with other interventions, could stop population declines.
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Scientific Reports, Vol. 9, no. 9158 (2019-06-24).

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1SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ Field trial of a probiotic bacteria to protect bats from white-nose syndromeJosephþ  R.þ  Hoytþ n þ n wáxზ‡þ  bäþ  Langwigþ n þ n xáräþ  ƒ—Žþ  Whiteyᇃ–Š‡”þ  äþ  ƒƒ”ƒƒyá Jenniferþ  A.þ  R edellyზ›þ  äþ þP Parisezá{ዐ‹ˆ”‡†þ  täþ  Fri …wá|ár‡¡”‡›þ  äþ  Fosterþ n þ n zá{ & äþ  ƒ”þ  ‹Ž’ƒ–”‹…w‘‘Ž•ˆ‘””‡†—…‹‰™‹Ž†Ž‹ˆ‡†‹•‡ƒ•‡‹’ƒ…–•ƒ”‡‡‡†‡†–‘…‘•‡”˜‡„‹‘†‹˜‡”•‹–›äŠ‹–‡æ‘•‡•›†”‘‡ fᅃ—•‡†„›–Š‡ˆ—‰—• Pseudogymnoascus destructans ኃ•…ƒ—•‡†™‹†‡•’”‡ƒ††‡…Ž‹‡•‹‘”–Š ‡”‹…ƒ„ƒ–’‘’—Žƒ–‹‘•ƒ†–Š”‡ƒ–‡••‡˜‡”ƒŽ•’‡…‹‡•™‹–Š‡š–‹…–‹‘ät‡™–‘‘Ž•‡š‹•–ˆ‘”ƒƒ‰‡”• –‘”‡†—…‡‹’ƒ…–•ä‡–‡•–‡†–Š‡‡•…ƒ…›‘ˆƒ’”‘„‹‘–‹…„ƒ…–‡”‹—á •‡—†‘‘ƒ•ª—‘”‡•…‡•á–‘ ”‡†—…‡‹’ƒ…–•‘ˆ‹–™‘•‹—Ž–ƒ‡‘—•‡š’‡”‹‡–•™‹–Š…ƒ‰‡†ƒ†ˆ”‡‡æª›‹‰ Myotis lucifugus „ƒ–•ƒ–ƒ‹‡‹‹•…‘•‹áäf–Š‡…ƒ‰‡‡š’‡”‹‡––Š‡”‡™ƒ•‘†‹¡‡”‡…‡‹•—”˜‹˜ƒŽ„‡–™‡‡ control and P. ª—‘”‡•…‡•æ–”‡ƒ–‡†„ƒ–•ä‘™‡˜‡”á„‘†›ƒ••á‘–‹ˆ‡…–‹‘‹–‡•‹–›á’”‡†‹…–‡† ‘”–ƒŽ‹–›á•—‰‰‡•–‹‰–Šƒ–™‹–Š‹æ…ƒ‰‡†‹•–—”„ƒ…‡‹ª—‡…‡†–Š‡…ƒ‰‡‡š’‡”‹‡–äf–Š‡ˆ”‡‡æª›‹‰ ‡š’‡”‹‡–ᙊ‡”‡„ƒ–•™‡”‡ƒ„Ž‡–‘ƒ˜‘‹†…‘•’‡…‹¤…†‹•–—”„ƒ…‡á‹ˆ‡…–‹‘‹–‡•‹–›’”‡†‹…–‡† –Š‡†ƒ–‡‘ˆ‡‡”‰‡…‡ˆ”‘–Š‡‹‡äf–Š‹•‡š’‡”‹‡––”‡ƒ–‡–™‹–Š P. ª—‘”‡•…‡• increased ƒ’’ƒ”‡–‘˜‡”™‹–‡”•—”˜‹˜ƒŽ¤˜‡æˆ‘Ž†…‘’ƒ”‡†–‘–Š‡…‘–”‘Ž‰”‘—’ˆ”‘~äz¬–‘z|äx¬f„›†‡Žƒ›‹‰ ‡‡”‰‡…‡‘ˆ„ƒ–•ˆ”‘–Š‡•‹–‡„›ƒ’’”‘š‹ƒ–‡Ž›yx†ƒ›•äŠ‡•‡”‡•—Ž–••—‰‰‡•––Šƒ––”‡ƒ–‡–‘ˆ„ƒ–• with P. ª—‘”‡•…‡•ƒ›•—„•–ƒ–‹ƒŽŽ›”‡†—…‡‘”–ƒŽ‹–›áƒ†á‹ˆ—•‡†‹…‘„‹ƒ–‹‘™‹–Š‘–Š‡” ‹–‡”˜‡–‹‘•á…‘—Ž†•–‘’’‘’—Žƒ–‹‘†‡…Ž‹‡•ä White-nose syndrome (WNS), caused by the introduced fungal pathogen, Pseudogymnoascus destructans, has caused widespread declines in bat populations throughout eastern and central North America and threatens several species with extinction1 – 6. ree species (Myotis lucifugus , Myotis sodalis , and Perimyotis subavus ) have declined by 70–90% across multiple states, and a fourth species, Myotis septentrionalis , has been extirpated from most sites within three years of WNS detection2,3,7, in part, due to highly connected bat communities8. Although a few populations of M . lucifugus appear to be persisting at 10–25% of pre-WNS colony sizes, most colonies of this species have declined by 90%7 , 9. Several previously common species of hibernating bats are now relatively rare across large regions of the northeast USA2 , 7 , 10. Management interventions to reduce the impact of WNS on bat populations are needed to prevent further declines and restore bat populations. Over the past seven years, several treatments for WNS have been explored and are in various stages of development. Potential treatments to enable bats to survive hibernation have included volatile compounds released by bacteria, vaccination, chemical anti-fungals, and probiotic microbes (TableS1). However, the results of most lab and eld trial studies have not been published, and there are currently no published reports of eective treatments from eld trials (TableS1). us, at present, there are few tools for managers to reduce the impacts of WNS, and developing control options to reduce the severity of this disease among bats is a high priority11,12. Our goal was to determine the ecacy of a probiotic bacterial treatment, Pseudomonas uorescens, in reducing WNS mortality in a eld setting. Pseudomonas uorescens is a ubiquitous bacterial species complex that is used as a fungal biocontrol agent in agriculture, and has been tested as a treatment for chytridiomycosis in amphibians13– 15. A previous study on multiple isolates of P . uorescens isolated from dierent species of bats showed w‡’ƒ”–‡–b…‘Ž‘‰›ƒ†b˜‘Ž—–‹‘ƒ”›‹‘Ž‘‰›á‹˜‡”•‹–›‘ˆƒŽ‹ˆ‘”‹ƒáƒ–ƒ”—œá{v|záäxDepartment ‹‘Ž‘‰‹…ƒŽ…‹‡…‡•á‹”‰‹‹ƒ‡…ŠáŽƒ…•„—”‰ááxzv|wáäyWisconsin Department of þNNatural Resources, Bureau ‘ˆƒ–—”ƒŽ‡”‹–ƒ‰‡‘•‡”˜ƒ–‹‘áƒ†‹•‘áfá{y}v}áäzDepartment of Molecular, þC Cellular & Biomedical …‹‡…‡á‹˜‡”•‹–›‘ˆ‡™ƒ’•Š‹”‡á—”Šƒáávy~xzáä{Present address: þC C enter for Microbial Genetics ƒ†n‡‘‹…•á‘”–Š‡””‹œ‘ƒ‹˜‡”•‹–›átŽƒ‰•–ƒ¡áá~|vwwáä|ƒ–‘•‡”˜ƒ–‹‘f–‡”ƒ–‹‘ƒŽá—•–‹á ‡šƒ•á}~}w|á䑔”‡•’‘†‡…‡ƒ†”‡“—‡•–•ˆ‘”ƒ–‡”‹ƒŽ••Š‘—Ž†„‡ƒ††”‡••‡†–‘rää䇏ƒ‹Žã hoytjosephr@ f‘”ää䇏ƒ‹Žã ) Received: 1 August 2018 Accepted: 3 June 2019 Published: xx xx xxxxþOPEOPEN


2SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ a range of antiP . destructans properties in vitro16. One strain, isolated from a hibernating Eptesicus fuscus in Virginia, reduced the number of lesions and increased survival of little brown bats when applied at the time of infection in a laboratory in vivo trial17. We performed a eld trial with two simultaneous experiments to balance the strengths and weaknesses of each approach. In the free-ying experiment, we treated bats and attached a passive integrated transponder (PIT) tag to each bat to determine the date it emerged from the site. is experiment allowed bats to move freely throughout the site and roost wherever they chose. However, there was additional uncertainty in determining the survival of free ying bats. Bats might die at the site and be eaten by predators, escape from the site by an unknown exit and not be detected by the PIT tag receiver, or move to another site midwinter and survive at that site. To balance these unknowns, we also performed an experiment with bats in cages. Placing bats in cages, as has been done in most in vivo experiments to date1 , 17– 19, prevents bats from leaving the site and provides certainty about the survival of each bat. However, caging bats alters their behavior (e.g. bats cannot select their roosting location and microclimate, and bats in the same cage may be disturbed when other bats arouse from hibernation20), and placing all bats in a treatment within a single cage results in pseudo-replication.‡•—Ž–•On the day of treatment, P . destructans infection prevalence, fungal loads and weights of bats were very similar among control and treatment groups in both experiments, suggesting that randomization of bats among treatment groups was successful (Fig. 1 ). In addition, infection prevalence and fungal loads in November were very similar to loads observed on M . lucifugus at other sites where the fungus has been present for at least one previous winter21,22.t”‡‡æª›‹‰‡š’‡”‹‡–äþ Of the 44 bats we treated, only one bat (from the control group) appeared to have le the site due to the disturbance of being handled/treated (it was detected by the PIT tag reader on the day of treatment November 20, 2015 and never again). We detected 13 of 29 bats in the control and P . uo rescens treatment groups on the PIT tag reader between December 9, 2015 and April 17, 2016. Six of seven P . uorescens -treated bats, and one of six control bats le the site on or aer the assumed overwinter survival date of March 8, 2016; the rest emerged earlier and we assumed they died (Fig. 2 ). We also found two additional bat carcasses from the control group inside the site. e fraction of bats known to be alive and detected by the PIT tag reader aer March 8 (apparent overwinter survival) was 46.2% (6/13) for P . uorescens-treated bats, which was signicantly higher than 8.5% (1/12) for control bats (Fig. 3 ; the remaining 7 bats had lost their PIT tag; see Methods). e last date a bat was detected on the PIT tag reader was over a month later for P . uorescens-treated bats than control bats, and, overall, was earlier for bats with higher fungal loads in November (Fig. 4 ). We did not compare dierences in fungal loads or UV-uorescence among treatment groups in March for bats in the free-ying experiment because only three bats were found and recaptured when we visited the site on March 8. e remaining bats were likely in dicult-to-access portions of the mine.ƒ‰‡‡š’‡”‹‡–äþ On March 8th, 2016, four of 15 (26%) bats in the P. uorescens cage and four of 15 (26%) bats in the control groupcage were still alive; the others were dead (Fig. 5 ). Survival to March 8 was higher for bats with higher November body mass (which did not dier between treatment groups; Fig. 1 ) (Fig. 6C). Unlike in the free-ying experiment, P . destructans fungal loads in November were not a signicant predictor of sur vival (Pþ t þ t 0.60). Most of the bats still alive in the cages were in very poor condition, and only one of the eight bats (from the control group) that survived to March 8 and was released was subsequently detected by the PIT tag reader (Fig. 5 ). Secondary measures of disease severity (Fig. 6A: fungal loads on bats in March and Fig. 6B: disease severity, as measured by UV-uorescence) from the cage experiment showed non-signicant dierences among groups.‹•…—••‹‘White-nose syndrome has caused widespread declines in multiple species of bats throughout eastern and central North America, with declines in M . lucifugus colonies in the rst year of WNS detection averaging 79%2 , 3. Survival of untreated bats in the free-ying experiment was similarly severe, with 91% (95% CI: 62, 99%) of control bats likely dying over the winter. In the free-ying experiment, treatment with the probiotic, P . uorescens, increased apparent overwinter survival more than ve-fold by extending the last date of detection by a month into early spring. Although over half of P. uorescens-treated bats still likely died from WNS over the winter, the eect of treatment was substantial. Our results suggest that P . uorescens could be a useful tool for reducing WNS impacts on bat populations, especially if treatment ecacy could be improved. One potentially important factor contributing to ecacy, based on work in other systems, is bacterial persistence and proliferation following treatment23. Increasing persistence and growth of P . uorescens on bats by altering the dosage, or treatment frequency, or adding components to P . uorescens solutions to encourage the formation of biolms could help increase treatment ecacy, assuming these alterations would not have deleterious side eects23. In addition, previous in vitro studies suggested that many dierent strains of P . uorescens have anti-P . destructans eects16. Future studies could examine alternate strains of P . uorescens or other bacteria isolated from dierent populations (e.g., persisting populations of M . lucifugus9) or other species of bats (the strain in this study was isolated from E. fuscus ;16). e eect of P . uorescens in reducing WNS impacts on other species has yet to be tested. e most impor tant species to protect from WNS is M . septentrionalis , which suers nearly 100% mortality, and is on a pathway toward extinction2. To date, no treatments have been developed for or tested on this species in either the lab or


3SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ eld. is is despite M . septentrionalis being the most heavily aected by WNS, with few hibernacula still containing this species in the US2,7,24. Our results oer potential insights for the experimental design of eld trials aimed at reducing WNS mortality in hibernating bats. Researchers oen have to choose between cage-artifacts and concerns about bats leaving the site following treatment and uncertainty in the survival outcome for free-ying bats. Our data suggest that the free-ying experiment was a better experimental design than the cage experiment, despite some challenges. Bats No ve mber 6 7 8 9 10 Mass (g) Tr eatmentExper iment P. fluorescenscage P. fluorescensfree flying Controlcage Controlfree flying A 0.00 0.25 0.50 0.75 Prev alenc e B 5 4 3 2 P. fluorescenscag eP . fluorescensfree flying Controlcage Controlfree flying T reatmen tlo g 10 Pd l o a d s C Figure 1.þ Bat body mass (A), P. destructans prevalence (B), and fungal loads (C) of M. lucifugus in November in two treatment groups (control (CO), and P. uorescens (PF). ere were 14–16 bats in each of the four treatment-experiment groups. ere were no signicant dierences among treatment groups in fungal loads, prevalence or body mass in November (likelihood ratio-tests for treatment eect in linear models of mass, generalized linear models with a binomial distribution for prevalence, and linear models for log-transformed fungal loads: all P-valuesþ t þ t 0.57).


4SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ in the free-ying experiment were able to roost and behave normally, and only one of 44 bats le the site on the day of the experiment, suggesting that the disturbance of handling and treatment are unlikely to compromise experiments if treatment can be done quickly (treatment, weighing and banding required ~65þ t minutes under ground in this study). In addition, apparent survival was higher for bats with lower November fungal loads (i.e., loads were positively correlated with emergence date), as would be expected if bats were dying from WNS24, suggesting that WNS was the dominant source of mortalityin the free-ying experiment. If bats were leaving the site due to disturbance, or simply to move between hibernacula, one would not expect a correlation between fungal loads and emergence date. e main challenge of the free-ying experiment was uncertainty associated with the fate of animals that were never detected by the PIT tag reader and not found dead within the site. However, as noted above, the extent of mortality in control bats inferred in the free-ying experiment was consistent with WNS declines observed in other populations of M . lucifugus . is supports the assumption that most bats that were not detected by the PIT tag reader aer March 8 did not survive the winter. One nal challenge with free-ying experiments is that treatment substances might be transferred among bats in dierent treatment 5 0 5 10Mean nightly temperature ( C)CO_1 CO_2 CO_3 CO_4 CO_5 CO_6 PF_7 PF_8 PF_9 PF_10 PF_11 PF_12 PF_13 No v Dec Ja nF eb MarA pr Last date detectedT reatment 5.5 5.0 4.5 4.0 3.5 3.0lo g10NovB at Loads Figure 2.þ External air temperature, emergence date and fungal loads of bats in the free-ying bat experiment. e top panel shows air temperature measured at the site entrance. In the lower panel, line color indicates the log10 P. destructans fungal load (in nanograms) measured on bats in November, with darker colors showing lower (more negative) fungal loads. Lines end on the last date that each bat was detected by the PIT tag reader, and dotted vertical lines show the mean emergence date for each treatment group, indicated on the Y-axis (Pf – P. uorescens, Co – Control). e gray vertical bar shows the date aer which emerging bats were assumed to have survived. 0.00 0.25 0.50 0.75 1.00 P. fluorescens ControlFraction Surviving Figure 3.þ Apparent overwinter survival of bats in the free-ying experiment. Columns show the fraction of bats in each treatment group that were known to be alive and detected by the PIT tag reader on or aer March 8 (with binomial 95% CI; sample sizes for each group, le to right, were 13, 12; the remaining 7 bats had lost their PIT tag; see Methods). Survival among treatments was signicantly dierent (P. uorescens – treated bats had higher apparent survival than the control group (Fisher’s exact test: one-tailed Pþ t þ t 0.046; logistic regression coef.þ t þ t 2.24þ t þ t 1.18, zþ t þ t 1.896, one-tailed Pþ t þ t 0.029).


5SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ groups (e.g., probiotic bacteria might be transferred from treated to control bats) and this could reduce the magnitude of treatment eects observed. e cage experiment suered from several shortcomings that, in hindsight, indicate this was a problematic design that should be modified if used in the future. In our experiment, we placed all the bats in each treatment into a single cage due to limited availability of space for mounting cages to natural substrate in a predator-protected room, and to allow social bats to roost in groups. is resulted in pseudo-replication in this experiment, as in most previous laboratory studies on WNS1 , 17– 19. is is particularly problematic for studies of WNS, because in small cages bats appear to disturb other bats when they arouse from hibernation20, and increased arousal frequency is thought to be a key mechanism of WNS mortality1 , 25, 26. e fact that survival in the cage experiment was correlated with initial body mass, but not fungal loads, suggests that disturbance from other bats, or an inability to move to other locations within hibernacula, was more important than WNS in determining survival in this experiment. Together, these results indicate that the ideal design for a eld trial (and for WNS challenge experiments more generally) is a free-ying experiment with mixed treatment groups in each site where bats have to pass through a PIT tag antenna to leave the site or are prevented from leaving the site (e.g. by sealing entrances, which may be very dicult). Sites where dead bats are relatively easy to nd and are not eaten by predators (e.g. mice, rats, raccoons, or skunks) would reduce uncertainty in survival outcomes. Alternatively, if the site entrance(s) cannot be adequately covered with a PIT tag antenna, one should use replicated cages (constructed of metal, to prevent mice from chewing into the cages) with a small number of bats in each cage (one for bats that usually roost alone; Ja n Fe b Mar Apr 5 4 3 November log10Pd loadsLast date detected PF CO Figure 4.þ November P. destructans fungal loads (in nanograms)and the last date of detection for individual bats in the two treatment groups (Pf – P. uorescens, Co – Control). P. uorescens-treated bats were detected later than Control bats, and the last date of detection decreased (was earlier) with higher November P. destructans loads (control vs. P. uorescens coefþ t þ t 1.05þ t þ t 0.58 months, tþ t þ t 1.91, one-tailed Pþ t þ t 0.050, early winter fungal loads: coefþ t þ t 0.66þ t þ t 0.34 months/ng P. destructans , tþ t þ t 1.93, one-tailed Pþ t þ t 0.041). CO_16 CO_17 CO_18 CO_19 CO_20 CO_21 CO_22 CO_23 CO_24 CO_25 CO_26 CO_27 CO_28 CO_29 CO_30 PF_31 PF_32 PF_33 PF_34 PF_35 PF_36 PF_37 PF_38 PF_39 PF_40 PF_41 PF_42 PF_43 PF_44 PF_45 Nov Dec Jan Feb Mar Apr DateTreatment 6789 10BatM ass Figure 5.þ Survival, subsequent detection, and body mass of bats in the caged bat experiment. Each horizontal line represents a single bat, with line color indicating the body mass of that bat in November. Bats surviving until release March 8 (gray vertical bar) are shown by darker lines, and the single bat that was subsequently detected by the PIT tag reader is extended to the last date it was detected. Treatment is indicated by the rst two letters of the bat identication number on the Y-axis (PF – P. uorescens, CO – Control).


6SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ a few for bats that roost in groups) to reduce cascading disturbances from infected bats. In addition, barriers to prevent larger predators (e.g. raccoons, skunks) from accessing the cages and eating the bats are an absolute necessity. Cages are not ideal in that they limit bats’ movement within sites, and individual bats within cages are pseudo-replicates (and thus need to be analyzed appropriately), but they oer higher certainty in terms of knowing the survival of each individual. In conclusion, our results suggest that P . uorescens could be a useful tool for reducing WNS impacts, and pairing P. uorescens treatment with other approaches could stop species declines due to WNS11. Potential strategies that could be combined with treatment include reducing the environmental reservoir of P . destructans3 , 27, protecting and facilitating growth of populations of M. lucifugus that are now persisting with WNS, and improving summer and fall habitat for bats to increase reproductive success and fat storage for hibernation that would enable bats to better tolerate infection21, 28. Increasing survival and reproduction could facilitate the evolution of resistance or tolerance which would eliminate the need for perpetual management action29. Finally, any strategy which slows or stops the very rapid local extirpations of M. septentrionalis colonies is urgently needed to prevent this species from extinction.‡–Š‘†•We performed the eld trial on M . lucifugus bats in the winter of 2015–16 at an inactive mine in southwest Wisconsin where P . destructans was detected the previous winter 2014–15. e mine has one large (~3þ t m tall by 5þ t m wide) entrance that was gated several years earlier, and a single smaller entrance that was sealed with a ne mesh metal screen the year of the gating. We selected a hibernacula where P . destructans had been detected the previous year because lab trials with P . uorescens indicated that treating bats at the time of infection was more benecial than treatment prior to infection17, and previous work suggests that most bats become infected early in the second year following P . destructans invasion, likely due to establishment of an environmental reservoir3. ere were 226þ t M . lucifugus at the site in November 2014, before P . destructans was detected, but the colony had declined to 82 bats by March 2015. e average temperature where M . lucifugus roosted at this site was 7.0þ t °Cþ t þ t 0.4þ t °C. We screened 55 samples for P . uorescens by PCR from bats collected in the winter of 2014–15 to conrm that P. uorescens naturally occurred on bats found in the site to address concerns regarding using a live bacterium as a treatment. We found DNA from P . uorescens or closely-related relatives present in 20% of samples and from all four species sampled (Little brown myotis (Myotis lucifugus ), Northern long-eared myotis (Myotis septentriona lis ), Tri-colored bat (Perimyotis subavus ), and Big brown bat (Eptesicus fuscus)). March 4 3 2 1 log 10 Pd loadsA 0.1 0.2 P. fluorescens Control T reatmentUV B No ve mber aliv ed ead 6 7 8 9 10 Tr eatment Mass (g) Tr eatment P. fluorescens Control C Figure 6.þ P. destructans (A) fungal loads, and (B) disease severity (UV) for bats in the cage experiment that survived to March 8, and (C) dierences in November mass between bats in the cage experiment that survived to March 8, or died. (A) March fungal loads did not dier among treatment groups (treatment eect in model of log-transformed fungal loads: Pþ t þ t 0.77). (B) Disease severity (ultraviolet uorescence) was not signicantly dierent for P. uorescens-treated bats than control bats (regression on arc-sin sqrt transformed data: control vs. P. uorescens: coefþ t þ t 0.017þ t þ t 0.073, zþ t þ t 0.234, Pþ t þ t 0.82). (C) Bats that were heavier in November had a higher probability of survival (logistic regression (reference group: control): Intercept: 20.1þ t þ t 7.0; Body mass: 1.22þ t þ t 0.44; Pþ t þ t 0.0054; P. uorescence coef. 0.40þ t þ t 0.97; Pþ t þ t 0.68).


7SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ In September 2015 we installed a PIT-tag reader (IS1001 and HPR reader, Biomark Inc., Boise, ID) at the site entrance (Fig.S1), and 3 metal screen cages (46þ t þ t 30þ t þ t 51þ t cm Fresh Air Screen Habitat, Zilla Products, Franklin, WI, USA) in a small chamber in the back of the mine where bats roosted in previous winters. We removed the top of each cage and mounted cages directly to the ceiling to allow bats direct access to the ceiling for roosting, and to allow for natural infection and reinfection. We also installed chicken wire with a hinged gate at the entrance of the cage room to prevent large predators (e.g. raccoons, skunks) from entering. We briey visited the site on Nov 16, 2015 (total time underground 14þ t minutes) to count the number of bats present and to assess the P . destructans -infection status of the bats at the site. We counted approximately 95þ t M . lucifugus and sampled six of them by dipping a sterile polyester swab in sterile water to moisten it and then rubbing the swab ve times across both the forearm and muzzle of a bat22. We tested these samples for P. destructans DNA using qPCR30, and all six samples tested positive. We returned to the site for the experimental treatment on Nov 20, 2015. We sealed o the entrance to the site (Fig.S1) using ne mesh cloth to prevent bats from leaving the site during the treatment. We collected all M . lucifugus we could nd at the site (89 bats; 23 females and 66 males) and placed them individually in paper bags and brought them to a processing station near the entrance of the site. We weighed bats to the nearest 0.1þ t g with an electronic scale. We did not take a length measurement (e.g. forearm) to minimize handling time and disturbance. Recent work has shown that body mass is equally accurate in predicting fat stores (as measured by quantitative magnetic resonance) as body condition indices31. We sampled bats for P . destructans as described above and banded each bat with an aluminum band (2.9þ t mm; Porzana Ltd., Icklesham, E. Sussex, U.K.), that had a PIT tag attached (see Supplemental Methods text). We randomly assigned bats to each of three treatment groups: control (Nþ t þ t 29 bats), P . uorescens (31 bats), and a third treatment that will be reported elsewhere (29 bats). We treated each bat by spraying ~2þ t ml of a solution containing P . uorescens (see Supplemental Methods text) on their wings and tail with a spray bottle (FantaSea, Blaine, WA). For control bats we replicated the handling disturbance but did not spray any liquid onto bats because our treatment could only be applied in liquid form and the goal of our study was to determine the eect of treatment compared to untreated bats. We split bats in each treatment group into the two experiments based on a power analysis (Fig.S2) – cage (15 for each treatment group in a single cage for each treatment; 5 females and 10 males per cage) and free-ying (16, and 14 bats in the P . uorescens and control groups, respectively). Aer treatment, bats were released into cages or into the site onto a recovery cloth ~75þ t m away from the processing station. We removed the mesh from the site entrance so bats could freely pass through the opening surrounded by the PIT tag antenna (Fig.S1). We blocked o the rest of the entrance with screening to discourage bats from attempting to leave the site without passing by the PIT tag antenna. e total time underground was 65þ t minutes. We returned to the site on March 8, 2016. We removed all the bats from the cages and captured all free-ying bats we could nd (some portions of the site are inaccessible). Each bat was swabbed as described above, and one wing was photographed under ultraviolet (UV) light to measure an index of disease severity32, 33. We then released all bats into the site. We downloaded data from the PIT tag reader on July 30, 2016 to determine the dates that bats with PIT tags were detected by the PIT tag reader. We note that detection by the PIT tag reader does not indicate the direction of travel when a bat is detected by the PIT tag reader (i.e. into or out of the mine). It only indicates that the bat was alive on that date and passed near (within ~15–20þ t cm) the PIT tag antennae. When processing bats from the cage experiment, we noted that ve of 16 (31%) of the PIT tags had become detached from the bands on the bats. As a result, we subsequently searched the site when no bats were present with a handheld PIT tag reader to determine whether free-ying had also lost their PIT tags. We found seven PIT tags that were not attached to bands, suggesting that known PIT tag loss in the free-ying group (seven of 24, or 29.2% of the bats that were never recorded on the PIT tag reader) was similar to that in the cage experiment (31%). ere may have been additional bats in the free-ying group that lost their PIT tags (making our survival calculations underestimates), but there is no evidence that PIT tag loss diered by treatment group (cage and free-ying groups combined: P . uorescens 4/31, control 4/29; Fisher’s exact test Pþ t þ t 0.93). We removed the bats who had lost their PIT tags (three P . uorescens, one control) from analyses for the free-ying group since we could not detect them on the PIT tag reader. We added a new band with a PIT tag to any bat from the cage trial that had lost its PIT tag or band. We determined the ecacy of the treatments by comparing apparent overwinter survival of bats in the treatments, with and without accounting for dierences in initial individual fungal loads and body mass. We assumed bats that were never detected by the PIT tag reader died in the site because our PIT tag reader antennae provided full coverage of the entrance and had sucient sensitivity to detect tags on ying bats. We assumed that any bats alive and detected by the PIT tag reader on or aer March 8, 2016 had survived the winter (which we term “appar ent overwinter survival”). We used March 8 as a cut-o for apparent overwinter survival because aer March 8, 2016 surface temperatures near the mine were consistently above 2þ t °C (Fig. 1 ). Bats detected by the PIT tag reader prior to March 8, and never again, were assumed to have died or permanently emigrated. We searched all known sites within 50þ t km for banded bats and did not nd any, suggesting that emigration to known sites was low. We compared the latest date a bat was detected by the PIT tag reader between treatments as a continuous response variable of the last date a bat was known to be alive, while controlling for fungal loads and mass measured in November. Finally, we examined dierences among treatment groups in UV uorescence among individuals surviving the cage experiment, as an indicator of disease severity. We hypothesized that bats treated with P . uorescens would have higher survival, have a later last detection by the PIT tag reader date, and lower UV uorescence than the control group. We further hypothesized that apparent overwinter survival would increase with initial body mass and lower initial fungal loads in both experiments. All research was performed as described under protocol Kilp1509 approved by the University of California, Santa Cruz’s IACUC committee. e work was exempt from FDA licensing, under regulation 21CFR 511.1.


8SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ ƒ–ƒ˜ƒ‹Žƒ„‹Ž‹–›e datasets generated during the current study are available from the corresponding authors on reasonable request.Referencesþt 1.þt Warnece, L. et al . Inoculation of bats with European Geomyces destructans supports the novel pathogen hypothesis for the origin of white-nose syndrome. Proc. Natl. Acad. Sci. USA 109, 6999–7003 (2012).þt 2.þt Langwig, . E. et al . Sociality, density-dependence and microclimates determine the persistence of populations suering from a novel fungal disease, white-nose syndrome. Ecol Lett 15, 1050–1057 (2012).þt 3.þt Langwig, . E. et al . Invasion dynamics of white-nose syndrome fungus, midwestern United States, 2012–2014. Emerging Infectious Disease 21, 1023–1026 (2015).þt 4.þt Hoyt, J. . et al . Widespread bat white-nose syndrome fungus, northeastern China. Emerg. Infect. Dis. 22, 140–142, https://doi. org/10.3201/eid2201.151314 (2016).þt 5.þt Hoyt, J. . et al . Host persistence or extinction from emerging infectious disease: insights from white-nose syndrome in endemic and invading regions. Proc. . Soc. B 283, 20152861, (2016).þt 6.þt Drees, . P. et al . Phylogenetics of a fungal invasion: Origins and widespread dispersal of white-nose syndrome. mBio 8, https://doi. org/10.1128/mBio.01941-17 (2017).þt 7.þt Fric, W. F. et al . Disease alters macroecological patterns of North American bats. Glob. Ecol. Biogeogr. 24, 741–749, https://doi. org/10.1111/geb.12290 (2015).þt 8.þt Hoyt, J. . et al . Cryptic connections illuminate pathogen transmission within community networs. Nature 563, 710–713, https:// (2018).þt 9.þt Langwig, . E. et al . esistance in persisting bat populations aer white-nose syndrome invasion. Philos. Trans. . Soc. B-Biol. Sci. 372, 20160044, (2017).þt 10.þt Fric, W. F. et al . An emerging disease causes regional population collapse of a common North American bat species. Science 329 , 679–682, (2010).þt 11.þt Langwig, . E. et al . Context dependent conservation responses to emerging wildlife diseases. Front. Ecol. Environ. 13, 195–202, (2015).þt 12.þt FWS, U. S. A National Plan for Assisting States , Federal Agencies, and Tribes in Managing White-Nose Syndrome in Bats, https://pubs. (2011).þt 13.þt Myers, J. M. et al . Synergistic inhibition of the lethal fungal pathogen Batrachochytrium dendrobatidis: the combined eect of symbiotic bacterial metabolites and antimicrobial peptides of the frog ana muscosa . J Chem Ecol. 38, 958–965, https://doi. org/10.1007/s10886-012-0170-2 (2012).þt 14.þt Gram, L., Melchiorsen, J., Spanggaard, B., Huber, I. & Al, G. Inhibition of Vibrio anguillarum by Pseudomonas uorescens AH2, a possible probiotic treatment of sh. Appl Environental Microbiol 65, 969–973 (1999).þt 15.þt Bangera, M. & Thomashow, L. Identification and characterization of a gene cluster for synthesis of the polyetide antibiotic 2,4-Diacetylphloroglucinol from Pseudomonas uorescens Q2-87. J. Bacteriol. 181, 3155–3163 (1999).þt 16.þt Hoyt, J. . et al . Bacteria isolated from bats inhibit the growth of Pseudogymnoascus destructans, the causative agent of white-nose syndrome. PLoS One 10, e0121329, (2015).þt 17.þt Cheng, T. L. et al . Ecacy of a probiotic bacterium to treat bats aected by the disease white-nose syndrome. J. Appl. Ecol. 54, 701–708, (2017).þt 18.þt Lorch, J. M. et al . Experimental infection of bats with Geomyces destructans causes white-nose syndrome. Nature 480, 376–378, (2011).þt 19.þt Johnson, J. S. et al . Host, pathogen, and environmental characteristics predict white-nose syndrome mortality in captive little brown myotis ( Myotis lucifugus ). Plos One 9, e112502, (2014).þt 20.þt Turner, J. M. et al . Conspecic disturbance contributes to altered hibernation patterns in bats with white-nose syndrome. Physiol. Behav. 140, 71–78, (2015).þt 21.þt Fric, W. F. et al . Pathogen dynamics during invasion and establishment of white-nose syndrome explain mechanisms of host persistence. Ecology 98, 624–631, (2017).þt 22.þt Langwig, . E. et al . Host and pathogen ecology drive the seasonal dynamics of a fungal disease, white-nose syndrome. Proceedings of the oyal Society B: Biological Sciences 282, 20142335, (2015).þt 23.þt Bletz, M. C. et al . Mitigating amphibian chytridiomycosis with bioaugmentation: characteristics of eective probiotics and strategies for their selection and use. Ecol Lett 16, 807–820 (2013).þt 24.þt Langwig, . E. et al . Drivers of variation in species impacts for a multi-host fungal disease of bats. Philos. Trans. . Soc. B-Biol. Sci. 371, 20150456, (2016).þt 25.þt eeder, D. M. et al . Frequent arousal from hibernation lined to severity of infection and mortality in bats with white-nose syndrome. Plos One 7, (2012).þt 26.þt Warnece, L. et al . Pathophysiology of white-nose syndrome in bats: a mechanistic model lining wing damage to mortality. Biology Letters 9, 20130177, (2013).þt 27.þt Hoyt, J. . et al . Long-term persistence of Pseudogymnoascus destructans, the causative agent of white-nose syndrome, in the absence of bats. Ecohealth 12, 330–333, (2015).þt 28.þt Cheng, T. L. et al . Higher fat stores contribute to persistence of little brown bat populations with white-nose syndrome. J. Anim. Ecol. 88, 591–600 (2019).þt 29.þt ilpatric, A. M. Facilitating the evolution of resistance to avian malaria in Hawaiian birds. Biological Conservation 128 , 475–485 (2006).þt 30.þt Muller, L. . et al . Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructans. Mycologia 105, 253–259, (2013).þt 31.þt McGuire, L. P. et al . Common condition indices are no more eective than body mass for estimating fat stores in insectivorous bats. J. Mammal. 99, 1065–1071 (2018).þt 32.þt Turner, G. G. et al . Nonlethal screening of bat-wing sin with the use of ultraviolet uorescence to detect lesions indicative of whitenose syndrome. J. Wildl. Dis. 50, 566–573, (2014).þt 33.þt McGuire, L. P. et al . White-nose syndrome disease severity and a comparison of diagnostic methods. Ecohealth 13, 60–71 (2016).…‘™Ž‡†‰‡‡–•Funding was provided by grants from Bat Conservation International, e Nature Conservancy, US Fish and Wildlife Service, and NSF (DEB-1115895 and DEB-1336290). We thank Andrew Badje, Tyler Brandt, Brian Heeringa, Maarten Vonho and Tim Carter for assistance in the eld, Samantha Sambado for processing the UV photos, and the Wagners for permission to use the site and logistical help.


9SCIENTIFIC REPOþrRTS | (2019) 9:9158 | Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæz{z{yæœ —–Š‘”‘–”‹„—–‹‘•J.R.H., A.M.K., K.E.L., J.P.W. designed the study. J.R.H., A.M.K., K.E.L., J.P.W., H.M.K., J.A.R. performed eld research. K.L.P. tested samples under J.T.F.’s supervision. J.R.H., A.M.K., K.E.L. performed analyses and wrote manuscript. All authors reviewed and provided comments on the manuscript.††‹–‹‘ƒŽfˆ‘”ƒ–‹‘Supplementary information accompanies this paper at Competing Interests : e authors declare no competing interests. Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional aliations. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. e images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit © e Author(s) 2019


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