Mosaic dental morphology in a terminal Pleistocene hominin from Dushan Cave in southern China


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Mosaic dental morphology in a terminal Pleistocene hominin from Dushan Cave in southern China

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Mosaic dental morphology in a terminal Pleistocene hominin from Dushan Cave in southern China
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Scientific Reports
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Liao, Wei
Xing, Song
Li, Dawei
Martinon-Torres, Maria
Wu, Xiujie et al
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Nature
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Biological Anthropology ( local )
Palaeontology ( local )
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Recent studies reveal high degrees of morphological diversity in Late Pleistocene humans from East Asia. This variability was interpreted as complex demographic patterns with several migrations and possible survival of archaic groups. However, lack of well-described, reliably classified and accurately dated sites has seriously limited understanding of human evolution in terminal Pleistocene. Here we report a 15,000 years-old H. sapiens (Dushan 1) in South China with unusual mosaic features, such as large dental dimensions, cingulum-like structures at the dentine level in the posterior dentition and expression of a “crown buccal vertical groove complex”, all of which are uncommon in modern humans and more typically found in Middle Pleistocene archaic humans. They could represent the late survival of one of the earliest modern humans to settle in an isolated region of southern China and, hence, the retention of primitive-like traits. They could also represent a particularity of this group and, hence, reflect a high degree of regional variation. Alternatively, these features may be the result of introgression from some late-surviving archaic population in the region. Our study demonstrates the extreme variability of terminal Pleistocene populations in China and the possibility of a complex demographic story in the region.
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Scientific Reports, Vol. 9, no. 2347 (2019-02-20).

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1SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš Mosaic dental morphology in a terminal þP Pleistocene hominin from Dushan Cave in southern ChinaWeiþ  Liaowáxᑐ‰þ  Xingyázკ‡‹þ  LiwáxეÀƒþ  Martinón-þT T orres{á|዗Œ‹‡þ  Wuyázá Christopheþ  þS Soligo{ár‘•±þ  ƒ”Àƒþ  Bermúd ez de Castro{á|ᇋþ  Wang} & Wuþ  LiuyázRecent studies reveal high degrees of morphological diversity in Late þPPleistocene humans from þEEast •‹ƒäŠ‹•˜ƒ”‹ƒ„‹Ž‹–›™ƒ•‹–‡”’”‡–‡†ƒ•…‘’Ž‡š†‡‘‰”ƒ’Š‹…’ƒ––‡”•™‹–Š•‡˜‡”ƒŽ‹‰”ƒ–‹‘•ƒ† ’‘••‹„Ž‡•—”˜‹˜ƒŽ‘ˆƒ”…Šƒ‹…‰”‘—’•ä‘™‡˜‡”ᎃ…‘ˆ™‡ŽŽæ†‡•…”‹„‡†á”‡Ž‹ƒ„Ž›…Žƒ••‹¤‡†ƒ†ƒ……—”ƒ–‡Ž› †ƒ–‡†•‹–‡•Šƒ••‡”‹‘—•Ž›Ž‹‹–‡†—†‡”•–ƒ†‹‰‘ˆŠ—ƒ‡˜‘Ž—–‹‘‹–‡”‹ƒŽŽ‡‹•–‘…‡‡ä‡”‡™‡ ”‡’‘”–ƒw{ávvv›‡ƒ”•æ‘Ž† H. sapiens —•Šƒwf‹‘—–ŠŠ‹ƒ™‹–Š——•—ƒŽ‘•ƒ‹…ˆ‡ƒ–—”‡•á•—…Š ƒ•Žƒ”‰‡†‡–ƒŽ†‹‡•‹‘•á…‹‰—Ž—æŽ‹‡•–”—…–—”‡•ƒ––Š‡†‡–‹‡Ž‡˜‡Ž‹–Š‡’‘•–‡”‹‘”†‡–‹–‹‘ ƒ†‡š’”‡••‹‘‘ˆƒò…”‘™„—……ƒŽ˜‡”–‹…ƒŽ‰”‘‘˜‡…‘’Ž‡šó჎Ž‘ˆ™Š‹…Šƒ”‡—…‘‘‹‘†‡” humans and more typically found in Middle þP Pleistocene archaic humans. þT They could represent the late •—”˜‹˜ƒŽ‘ˆ‘‡‘ˆ–Š‡‡ƒ”Ž‹‡•–‘†‡”Š—ƒ•–‘•‡––Ž‡‹ƒ‹•‘Žƒ–‡†”‡‰‹‘‘ˆ•‘—–Š‡”Š‹ƒƒ†á Š‡…‡á–Š‡”‡–‡–‹‘‘ˆ’”‹‹–‹˜‡æŽ‹‡–”ƒ‹–•äŠ‡›…‘—Ž†ƒŽ•‘”‡’”‡•‡–ƒ’ƒ”–‹…—Žƒ”‹–›‘ˆ–Š‹•‰”‘—’ ƒ†áŠ‡…‡á”‡ª‡…–ƒŠ‹‰Š†‡‰”‡‡‘ˆ”‡‰‹‘ƒŽ˜ƒ”‹ƒ–‹‘äŽ–‡”ƒ–‹˜‡Ž›á–Š‡•‡ˆ‡ƒ–—”‡•ƒ›„‡–Š‡”‡•—Ž– of introgression from some late-surviving archaic population in the region. þO Our study demonstrates –Š‡‡š–”‡‡˜ƒ”‹ƒ„‹Ž‹–›‘ˆ–‡”‹ƒŽŽ‡‹•–‘…‡‡’‘’—Žƒ–‹‘•‹Š‹ƒƒ†–Š‡’‘••‹„‹Ž‹–›‘ˆƒ…‘’Ž‡š demographic story in the region. As a key region of Late Pleistocene human evolution, East Asia has for the last ten years been the source of remarkable new insights into the origin, evolution and interaction of modern humans. A series of recent studies revealed that the morphology and evolutionary patterns of Late Pleistocene humans in East Asia are more complicated than previously documented1 – 3. e discovery and study of the Late Pleistocene hominin fossils of the Huanglong Cave, Zhirendong, Daoxian and other sites in Southern China indicate that modern humans appeared in the region as early as 120–80 ka4 – 7 increasing the time of possible overlap with other archaic hominins. e study of the hominin fossils recovered at Xujiayao and the new ndings from the Xuchang site reveal that in the transition from the late Middle to the early Late Pleistocene North China was inhabited by some primitive populations with unclear taxonomic assignment8 – 10. e fossil discoveries and related studies of H. oresiensis11, 12 at Liang Bua Cave on Flores, Indonesia, and the fossil and genetic data recovered at Denisova Cave in southern Siberia13,14 further support the late survival of some archaic populations that likely overlapped in time and geography with modern humans in East Asia. In addition, the genetic analysis of the Denisovans and the individual from the Tianyuan Cave revealed possible interbreeding of these hominins with Neanderthals, early modern humans or even archaic populations in or around Oceania14–16. In the general context of a taxonomically more diverse scenario, the morphology and evolution of hominins in the late part of the Late Pleistocene, in particular, have been the subject of increasing attention. Both morphological and genetic analyses of the populations that inhabited East Asia during this period reveal a high diversity that has been interpreted as evidence of a coexistence of more than one hominin lineage and/or the wState Key Laboratory of Geological Processes and Mineral Resources, School of þEEarth Sciences, þC China University of n‡‘•…‹‡…‡•á—Šƒázyvv}zኋƒäx–Š”‘’‘Ž‘‰›—•‡—‘ˆn—ƒ‰š‹áƒ‹‰á{yvvx~án—ƒ‰š‹áŠ‹ƒäyKey Laboratory of Vertebrate þEEvolution and Human Origins of þC Chinese Academy of Sciences, þII nstitute of Vertebrate ƒŽ‡‘–‘Ž‘‰›ƒ†ƒŽ‡‘ƒ–Š”‘’‘Ž‘‰›áŠ‹‡•‡…ƒ†‡›‘ˆ…‹‡…‡•á‡‹Œ‹‰áwvvvzzኋƒäzþCC AS þCCenter for bš…‡ŽŽ‡…‡‹‹ˆ‡ƒ†ƒŽ‡‘‡˜‹”‘‡–ᇋŒ‹‰áwvvvzzኋƒä{Department of Anthropology, University ‘ŽŽ‡‰‡‘†‘fáwzƒ˜‹–‘–”‡‡–ᑐ†‘áwváä|þNNational Research þC Center on Human þEEvolution bfbfვ‡‘‹‡””ƒ†‡–ƒ’—‡”…ƒ•á—”‰‘•ávvvxᒃ‹ä}þIInstitute of þC Cultural Heritage, Shandong University, }xr‹‘拐Šƒ‹‘ƒ†á‹‰†ƒ‘áx||xy}ኋƒä‘””‡•’‘†‡…‡ƒ†”‡“—‡•–•ˆ‘”ƒ–‡”‹ƒŽ••Š‘—Ž†„‡ƒ††”‡••‡†–‘ W.W. (email: wangw@sdu.edu.cn ) or W.L. (email: liuwu@ivpp.ac.cn ) Received: 18 January 2018 Accepted: 18 December 2018 Published: xx xx xxxxþOPEOPEN

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2SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš interbreeding between hominin groups15– 18. Recent analyses of both mitochondrial and Y chromosome DNA revealed high levels of genetic diversity in the populations of southern China, especially in Southwest China, with persistence of some novel basal haplogroups that directly emanate from the Eurasian founder nodes. Subsequent analyses suggested that these newly identied basal lineages likely represent the genetic relics of modern humans initially peopling East Asia instead of being the results of gene ow from the neighboring regions. is diver sity was caused by either novel indigenous haplogroups or “foreign” lineages introduced by the ancient and/or recent migration event(s). Southwest China was likely the genetic reservoir of modern humans aer they entered East Asia19, 20. According to some researchers, the morphology of the terminal Pleistocene human fossils from Maludong and Longlin, both in Southwest China could be indicative of complex demographic patterns, with several human migrations and possible survival of archaic populations21– 23 although their relationship with other Late Pleistocene-Holocene populations from mainland Southeast Asia remains unclear24, 25. In addition, the identication of ancient mt-DNA and Y-DNA lineages in Southwest China has led to the denition of this region as a “hotspot of human diversity”19, 20, 23. Despite these advances, the detailed morphological pattern, diversity and evolutionary implications of the terminal Pleistocene hominins in Southern China need further investigation. e lack of a substantial number of well-described, reliably classied and accurately dated sites has seriously limited our understanding of human evolution in the Pleistocene-Holocene transition in southwest China. In the present study, we aim to contribute to this topic by reporting a recently discovered human dentition from Dushan Cave, southern China. Dushan Cave is located in Linfeng Town, Tiandong County, Guangxi Zhuang Autonomous Region in South China. Excavation at Dushan Cave in 2011 unearthed some human remains, a rich assemblage of stone tools and some faunal remains (see SI-1 for details). e human fossils found at Dushan Cave include a partial cranium, mandibular fragments, and complete upper and lower dentitions except for the le M3 (Fig. 1 , SI-Fig.3 and SI-Table2). All the fossils belong to the same individual (Dushan 1). AMS radio carbon dating plus geology and faunal analysis indicate that Dushan 1 lived in this area during the period from ~15,850 to ~12,765 years BP (see SI-1 for details). In the present study, the metrics and both the external (outer enamel surface, OES) and internal (enamel dentine junction, EDJ) morphology of Dushan 1 are collected and compared to a large sample of fossil and contemporary Homo sapiens as well as some archaic Homo populations from East Asia.Resultse analysis and comparisons of the metric and non-metric traits of Dushan 1 teeth with those of various fossil hominins and recent modern humans in the present study indicate that both the maxillary and mandibular dentitions of Dushan 1 exhibit a mosaic morphological pattern combining primitive and derived traits. Nearly all the primitive dental traits identied in Dushan 1 resemble those of Middle Pleistocene Homo erectus and are unusual and/or absent in H. sapiens. Apart from the preliminary evaluation of the skull and the mandible, Dushan 1 teeth also present some derived features that are typically found in H. sapiens26–28. Our study of the dental morphology of Dushan 1 suggests that it represents a Homo sapiens individual with an unusual retention of ancestral traits.Dental traits typical of H. sapiens ‹—•Šƒwäþ In the present study, each individual tooth of Dushan 1 was described and compared (SI-4, SI-5, SI-Table9) with particular attention to a selection of 69 metric and non-metric traits listed in SI-Table10. Below we further summarize the expression of the most relevant ones (see also Table 1) pointing to anities with modern humans. Figure 1.þ e maxillary and mandibular dentitions of Dushan 1. (A) le maxillary dentition; (B) right maxillary dentition; (C) right mandibular dentition; (D) le mandibular dentition. From le to right: I1 to M3. Le M3 was missing).

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3SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš In general, both upper and lower anterior teeth of Dushan 1 are gracile and simple in their morphology (Fig. 1 , SI-Figs3, 7, 8, 10 and 11) resembling those of H. sapiens . Except the upper central incisors, all other incisors of Dushan 1 (I2s, I1s and I2s) exhibit weakly developed double shoveling which is usually regarded as autapomorphic of H. sapiens26– 28. e interruption groove found in the I2 of Dushan 1 is also a typical modern human feature27 Dushan 1 East Asia archaic Homo East Asia EMH East Asia RMH Java H. erectus Neanderthal Global H. sapiens Trait comparisons (Frequency and specimen number) 3 I1 Labial convexity 1/2 (EP-2) 92.8% (13/14) 42.9% (3/7) 9.6% (8/83) 100% Nþ t þ t 21 30.8% Nþ t þ t 91 6 I2 Interruption groove present 1/2 (Hs) 0% (0/7) 0% (0/6) 43.6% (92/211) 10.4–65.0% Nþ t þ t 5693 9 P3 Transverse crest present 0/2 (Hs) 17.7% (3/17) 20% (1/5) 4.2% (3/71) 33.3% (3/9) 6.7% Nþ t þ t 15 2% (6/283) 10 P4 Transverse crest present 0/2 (Hs) 21.4% (3/14) 25% (1/4) 2.9% (2/70) 50% (4/8) 12.4% Nþ t þ t 16 2% (5/279) 13 P3 Bifurcated essential crest 2/2 (EP-2) 100% (15/15) 0% (0/4) 44.2% (23/52) 53.3% Nþ t þ t 15 11.1% Nþ t þ t 126 14 P4 Bifurcated essential crest 2/2 (EP-2) 100% (16/16) 55.3% (26/47) 78.6% Nþ t þ t 14 9.4% Nþ t þ t 106 17 P3 Mesial or distal accessory ridge 2/2 (Hs) 93.3% (14/15) 50% (2/4) 43.3% (26/60) 36.4% Nþ t þ t 11 23.4–57.0% Nþ t þ t 749 18 P4 Mesial or distal accessory ridge 2/2 (Hs) 93.8% (15/16) 67.6% (46/68) 41.7% Nþ t þ t 12 33.0–80.2% Nþ t þ t 749 19 P3 Mesial or distal accessory ridge 2/2 (Hs) 100% (10/10) 25% (1/4) 52.0% (40/77) 90% Nþ t þ t 20 11.9% Nþ t þ t 13521 20 P4 Mesial or distal accessory ridge 2/2 (Hs) 100% (8/8) 33.3% (1/3) 46.6% (34/73) 70% Nþ t þ t 20 23% Nþ t þ t 115 21 P3 Accessory marginal tubercle 2/2 (EP-2) 93.3% (14/15) 25% (1/4) 19.2% (10/52) 22 P4 Accessory marginal tubercle 2/2 (EP-2) 93.8% (15/16) 14.9% (7/47) 24 P4 Two-rooted premolars 2/2 (EP-2) 40% (2/5) 0% (0/6) 4.3% (2/47) 27 ree-rooted P32/2 (EP-1) 0% (0/9) 0% (0/5) 0% (0/46) 0% (0/599) 28 P3 Tomes’s Root 2/2 (EP-2) 64.8% (142/219) 19.9% Nþ t þ t 1371 39 M1 Carabelli’s trait (Dushan 1 has special expression) 2/2 (EP-1þ t EP-2) 45% (9/20) 21.4% (3/14) 9.7% (27/279) 100% (2/2) 50% Nþ t þ t 20 1.9–36.0% Nþ t þ t 2426 49 M1 protostylid (Dushan 1 has special expression) 2/2 (EP-1þ t EP-2) 53.9% (7/13) 18.2% (2/11) 18.3% (63/345) 3.2% Nþ t þ t 31 18.7% Nþ t þ t 2362 60 ree-rooted M12/2 (Hs) 7.7% (1/13) 0% (0/15) 24.2% (15/62) 0–31.1% 62 Complex occlusal morphology at EDJ of molars 5/5 (EP-2) 100% (16/16) 15.2% (5/33) 5.2% (8/153) 63 Buccal basal swelling C1M3C1M32/2 2/2 (EP-2) 54.6–63.6% Nþ t þ t 19 55.6–88.9% Nþ t þ t 22 14.6% Nþ t þ t 82 12.9% Nþ t þ t 70 0–25.7% Nþ t þ t 101 0–12.9% Nþ t þ t 126 65 Crown buccal vertical groove complex Present (Uniqueþ t þ t EP-1) Absent Absent Absent Nþ t þ t 5 Weakly expressed (La Quina H18) 66 Tooth size proportions I1-C1/P3-M30.32 (Hs) 0.40 0.37 0.37 0.42 0.36 67 Tooth size proportions I1-C1/P3-M30.26 (Hs) 0.27 0.25 0.26 0.31 0.26 68 Molar size % increase M1– M20.5/2.6 Nþ t þ t 2 (EP-2) Meanþ t þ t 1.4 11.5/6.3 Nþ t þ t 6 Meanþ t þ t 1.2 3.1/9.9 þ t Nþ t þ t 8 Meanþ t þ t 5.0 14.2/4.8 Nþ t þ t 174 4/9 Nþ t þ t 5 15/5 N þ t þ t 250 69 Molar size % increase M2– M31.3/0.9 Nþ t þ t 2 (EP-2) Meanþ t þ t 2.8 13.1/6.3 Nþ t þ t 5 Meanþ t þ t 3.7 6.4/4.7 Nþ t þ t 5 Meanþ t þ t 2.6 18.9/18.0 Nþ t þ t 124 10/0 Nþ t þ t 5 15/14 Nþ t þ t 211Table 1.þ e frequency and expressions of key dental traits in Dushan 1 and comparative samples. Morphological status of Dushan Cave in the parentheses. ‘EP-1’: a primitive condition shared with the Africa Austrapithecus and early Homo . ‘EP-2’: a primitive condition shared with the Eurasia archaic Homo . ‘Hs’: a derived condition shared with H. sapiens. ‘Hs-Eu’: a derived condition shared with European H. sapiens or Neanderthal. ‘unique’: a unique condition restricted to Dushan Cave.

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4SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš and to our knowledge, it has not been found in any Middle Pleistocene or Late Pleistocene Homo from East Asia but is particularly frequent in modern humans in this region (Table 1). All the upper and lower premolars of Dushan 1 exhibit well-developed mesial or distal accessory ridges (Fig. 3 , Table 1 , SI-Table10). is feature is found in nearly all the Middle Pleistocene Homo specimens from East Asia and Neanderthals. In Late Pleistocene early modern humans, the frequencies of mesial or distal accessory ridges in both upper and lower premolars decrease to about 50%. Our observations indicate that 43–67% of upper and lower premolars of East Asian recent modern humans (RMH) have this feature. e frequencies of this trait in global RMH populations reported by Burnett and colleagues29 are 23.4–57.0% and 33.0–80.2% for P3 and P4, respectively. e frequencies they report for East Asian populations are even higher (50.7–80.2%). Considering both fossil hominins and recent modern humans have higher frequencies of the accessory ridge in premolars, we tentatively consider the occurrence of this trait in the premolars of Dushan 1 as resemblance to H. sapiens. In addition, there are other traits in the postcanine dentition of Dushan 1 that are more frequently observed in H. sapiens such as the lack of transverse crests in both upper and lower premolars30,31 or the three-rooted M1, a typical H. sapiens feature with higher frequencies in East Asian populations27,32.”…Šƒ‹…†‡–ƒŽ–”ƒ‹–•‹—•Šƒwäþ In addition to the modern dental traits mentioned above, Dushan 1 upper and lower dentitions display more archaic traits that are not usually found in modern humans (see Table 1 for details). Crown size.þ e crown sizes of Dushan 1 upper and lower teeth are very large compared to those of Middle Pleistocene archaic Homo , early modern humans and recent modern humans from East Asia. All the mesiodistal (MD) and buccolingual (BL) crown dimensions of Dushan 1 (except for the BL dimensions of I1 and I2) exceed the diameters of Late Pleistocene early modern humans and Holocene modern humans and, in some dimensions, they are close to or even exceed the values of Asian Middle Pleistocene archaic Homo (Fig. 2 and SI-Table10). Relative tooth size dierences between Dushan 1 individual and comparative samples including Neanderthals, early and recent modern humans from China, the Levant, North Africa and the Canary Islands, are further conrmed by Principal Component Analysis (PCA) of crown dimensions across dental classes in both upper and lower dentition (see SI-4 for details). Indices of tooth size proportions between anterior and posterior teeth also exhibit a similar trend (Table 1 and SI-Table10), and reveal the resemblance of Dushan 1 to modern humans in its anterior teeth18. Additional analysis of both bivariate plots and PCA of the bucco-lingual widths of upper and lower I2, P3, and M1 of Dushan 1 and some specimens that preserve the three dental classes also reveal the same trend (see SI-4 for details). Molar size proportions.þ Previous studies indicate a marked size reduction of the molar series during the course of evolution of the genus Homo , which caused the molar size sequence to change from a primitive pattern of ‘M1þ t þ t M2þ t þ t M3’ to a derived pattern of ‘M1þ t þ t M2þ t þ t M3’33, 34. In the present study, we compare the lower molar crown size proportions (square root of the calculated crown area [MDþ t þ t BL]) from M1 to M2 ([M2 M1]/M1), and from M2 to M3 ([M3 M2]/M2) in Dushan 1 and comparative samples following the approach of Kaifu31. As shown in Table 1 , there is only a slight size increase or decrease from M1 to M2 ( 0.5% toþ t 2.6%) and from M2 to M3 ( 1.3% to 0.9%) in Dushan 1. Compared to those of the fossil hominins and recent modern humans (Table 1 , SI-Table10), the molar size portions of Dushan 1 exhibit the primitive status relative to the Late Pleistocene early modern humans and resemble Middle Pleistocene archaic Homo . I1 Labial convexity.þ Moderate to pronounced labial convexity occurs more oen in earlier hominins, including Middle Pleistocene hominins and Neanderthals (Table 1 , SI-Table10)27, 30, 33. H. sapiens is the only group that Figure 2.þ Mesiodistal (MD) and buccolingual (BL) dimensions of Dushan 1 teeth and comparative samples from East Asian Pleistocene Homo and recent human populations.

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5SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš displays completely at labial surfaces (ASUDAS grade 0), although early H. sapiens such as Qafzeh or Dolni Vestonice may show labial surfaces with more pronounced grades than contemporary humans. For East Asian Pleistocene hominins, the frequencies of the I1s labial convexity decreased from 92.8% (Middle Pleistocene archaic Homo ) to 42.9% (early modern humans) ( grade 2). Our observation shows the le and right I1s of Dushan 1 exhibit weak and trace labial convexity respectively (ASUDAS grades 2 and 1). But more pronounced labial convexities are also identied in the C1s of Dushan 1 (ASUDAS grade 4) (Fig. 1, SI-Figs3, 7 and 8). Bifurcated essential crest (P3and P4).þ e bifurcated essential crests for P3 and P4 are more frequently observed in earlier hominins30 , 35 , 36. Our data for East Asian Pleistocene hominins show that bifurcated essential crests of P3 and P4 only occurred in Middle Pleistocene archaic Homo . Bifurcated essential crests of upper premolars are also found in some Middle Pleistocene populations from Europe (like Atapuerca SH) and Neanderthals30. In our small sample of recent modern Chinese humans (52 and 47 for P3 and P4, respectively), bifurcated essential crest can be observed in 44.2% and 55.3% of the specimens (Table 1 , SI-Table10), while the frequencies of this feature in the P3 and P4 of global recent modern humans are only 11.1% and 9.4% respectively30. erefore, the presence of bifurcated essential crests in both P3 and P4 of Dushan 1 (Fig. 3) may represent a primitive condition or a feature particularly more frequent in China since early times. P3 and P4 accessory marginal tubercle.þ Over 90% of East Asian Middle Pleistocene Homo specimens exhibit accessory marginal tubercles in their upper premolars. e occurrence of this feature in P3s of early modern humans is only 25%. e frequencies of accessory marginal tubercles in upper premolars of recent modern humans are even lower (19.2% and 14.9% respectively). is feature can also be observed in Dmanisi and Middle Pleistocene European hominins. All the Dmanisi upper premolar (P3 of D3672 and two P4s of D2700 and D2282) have their central grooves bifurcating before reaching the marginal ridges resulting in mesial and distal accessory tubercles37. e accessory marginal tubercles are observed in 94.3% of the Atapuerca SH upper premolars30. us, the presence of accessory marginal tubercles in Dushan 1 P3 and P4 can be regarded as a primitive feature (Fig. 3, Table 1, SI-Table10). Upper molars Carabelli’s trait.þ All the ve upper molars of Dushan 1 exhibit Carabelli’s trait with more pronounced expressions at EDJ level (Fig. 4 ). Carabelli’s trait frequencies have high intraand inter-population Figure 3.þ Some morphological traits identied in Dushan 1 teeth. (A) buccal view of right P4. Arrow indicates the buccal vertical groove. (B) Distal view of right P3 showing the divergent roots and basal bulging. (C) Occlusal view of right P3. (D) Occlusal view of le P4. (E) Buccal view of le P3. Arrow indicates the buccal groove. ( F) Occlusal view of le C1. (G) Occlusal view of right P3. (H) Occlusal view of le P4. Note the pronounced crown buccal vertical grooves, basal bulging and complicated occlusal morphologies in all teeth. (All the EDJ images are not scaled).

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6SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš variability so the taxonomic utility of its prevalence is limited33. However, there are some important dierences in the pattern of expression of Carabelli’s trait38,39. In general, even in the most pronounced cases, Carabelli’s trait in modern humans still express as the shape of a cusp that can sometimes express a free apex. However, in earlier fossil hominins and in non-human primates, Carabelli’s trait tends to adopt the shape of a cle or cingulum-like feature that commonly exceeds the protocone surface30,36–40. It has been suggested that Carabelli’s trait is a cingulum remnant on hominin upper molars38, 39. e frequencies of occurrence of Carabelli’s trait in modern human populations are low, ranging between 1.9–36.0% with 9.7%27,33 in East Asian RMH (Table 1, SI-Table10). e Carabelli’s trait expression is one of the most remarkable features of Dushan 1 teeth. All the upper molars of Dushan 1 have a deep and pronounced groove running along the upper part of the lingual side, near the occlusal edge of the protocone and the hypocone. Carabelli’s trait is also found in Chinese Middle Pleistocene Homo erectus of Hexian, Zhoukoudian and Yiyuan. e Carabelli’s trait all express as pits, Y-shaped depressions or small cusps in these specimens (SI-Fig.13). ey are limited to the lingual surface of the protocone, and are not as pronounced or uniquely expressed as in Dushan 141, 42. In Javanese Homo erectus, Carabelli’s trait usually weakly express as narrow pits or Y-shaped depressions (grade 4 in Ortiz’s standard)43,44. It is noteworthy that the expression of Carabelli’s trait in Dushan 1 strongly resembles that of the late-Middle or early-Late Pleistocene archaic human from Xujiayao. e Xujiayao M1 and M2 (PA1480-5 and PA1480-6) also exhibit the horizontal groove-like or shelf-like Carabelli’s cusp complex involving both protocone and hypocone (SI-Fig.13)8. A recent study of the Denisovan M2 (Denisova 4) shows that this tooth exhibits a similar Carabelli’s trait expression with a massive horizontal groove with multiple apexes occupying the whole base of the protocone and crossing the mesio-lingual groove. is expression pattern was regarded as a manifestation of rudimentary derivatives of the cingulum45. Carabelli’s trait in Australopithecus is more oen expressed as a composite feature formed by the lingual cingulum and wrinkled enamel associated with one or more cuspules, which usually involves the lingual surface of both protocone and hypocone. e lingual surface of A. africanus upper molars generally exhibits a partial or complete shelf-like cingulum, whereas that of P. robustus more frequently possesses a deep but small pit and/or one or more shallow grooves38–40. e shelf-like Carabelli’s cusp extending across the lingual surface of both the protocone and the hypocone in Dushan 1 diers from that of most Asian Middle Pleistocene hominins but resembles that of the Chinese archaic hominin from Xujiayo and possibly Denisovans. We have not found a shelf-like Carabelli’s trait in any H. sapiens upper molars. However, the scarcity of EDJ data for a wide and geographically comprehensive H. sapiens sample obliges us to be cautious about its interpretation. Overall, we consider that the expression of a “shelf-like Carabelli’s trait” in Dushan 1 suggests retention of a primitive feature. Protostylid.þ Just as Carabelli’s trait in upper molars, the protostylid is also regarded as a cingular derivative33, 46– 48. All lower molars of Dushan 1 exhibit a pronounced protostylid, which is more pronounced still at the EDJ surface (Fig. 5 , SI-Fig.14). Early hominins usually have higher frequencies and more pronounced expressions of this trait. About 50% of the Australopithecus and early Homo members of Africa show this feature46– 48. East Asian Middle-Late Pleistocene archaic Homo also exhibit higher frequencies of a protostylid. e frequencies of this feature in early modern humans and recent modern humans are within 20% (Table 1, SI-Table10). Interestingly, the dierences among hominins are not only regarding the frequencies but also in their form. In Late Pleistocene and contemporary modern humans, the protostylid is usually associated with the buccal groove. But in earlier hominins such as Asian H. erectus or the Dmanisi hominins, the protostylid is more cingulum-like, expressed as a cle or badge that extends across most of the buccal surface of the molar37. is pattern of expression is even more pronounced at EDJ level47, 48. As with the Carabelli’s trait, the protostylid of Dushan 1 is very pronounced and cle-like involving nearly the whole upper buccal surface of protoconid and hypoconid. We consider that the morphology of the protostylid of Dushan1 represents a primitive feature shared with some Middle Pleistocene Homo in Eurasia. Figure 4.þ Lingual view of Dushan 1 right upper molars showing cingulum-like Carabelli’s cusps.

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7SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš ree-rooted P3.þ Previous studies indicate that upper and lower premolars tend to reduce their root number throughout the evolution of the genus Homo . Nearly all the Australopithecus species have two-rooted P3s. 55.6% of African early Homo ( Homo habilis )49 and about 50% of Homo erectus specimens from Java have clearly bifur cated external roots of P3s32. So far, we have not identied any two-rooted P3s in the Chinese Middle Pleistocene Homo record although a few Sinanthropus P3s (nos. 21, 23, 82) from Zhoukoudian exhibit Tomes’ roots50. e P3s of European Early Pleistocene hominins are all two-rooted with either Tomes’ root (Sima del Elefante) or a clear bifurcation corresponding to a 2þ t R: MBþ t þ t DL pattern (Gran Dolina-TD6: H. antecessor ) following Wood’s standard (49 and authors’ observations of the ATD6-96 with micro-CT scanning). European Middle Pleistocene hominins and Neanderthals usually have single-rooted P3s30,51,52. e frequencies of two-rooted P3s in global modern humans are very low, ranging from 1.0% to 6.6% with average of 3.6%53,54. ree-rooted P3s are very rare in both fossil hominins and recent modern humans27, 30, 32, 33, 54. is feature was observed in some non-human primates like Pan , Gorilla, Pongo and Hylobates with frequencies ranging from 1–50%32. For fossil hominins, three-rooted P3s were only found in Australopithecus africanus , Australopithecus afarensis and Paranthropus robustus32, 55. e three-rooted P3s in modern human populations are even rare with reported occurrence in global modern human populations as 0.2% (1/584)54. e micro-CT observation of both Dushan P3s reveal that they are three-rooted with a 3-C-1 form as dened by Moore (ref.55; Fig. 6). us, the three-rooted P3s observed in Dushan 1 represent the retention of another primitive feature in this specimen. Two-rooted P4.þ As shown in Fig. 6 , both P3s and P4s of Dushan 1 are two-rooted, with a pronounced bifurcation. Most upper premolars of Middle Pleistocene hominins are two rooted. For example, 64.3% and 87.5% of the Atapuerca SH P3s and P4s are two-rooted, respectively30. However, two-rooted P4s are very infrequent in Late Pleistocene and contemporary modern human groups, ranging from 0% to 4.3% in our Late Pleistocene and Chinese modern sample, respectively33. erefore, a two-rooted P4 is an additional primitive trait in Dushan 1, more commonly found in Middle Pleistocene hominins. Complex occlusal morphology at EDJ of molars.þ Micro-CT scanning shows that the EDJ of all the post-canine teeth of Dushan 1 is characterized by complex patterns of ridges, furrows and crenulations. is is particularly pronounced in upper and lower molars (Fig. 7 , SI-Fig.15). Recent studies of East Asian Middle Pleistocene Homo populations (Xujiayao, Hexian, Yiyuan, Zhoukoudian) reveal that all these hominins exhibit complex EDJ mor phologies in their post-canine teeth coined as “dendrite-like” EDJ8 , 41, 42. is “dendrite-like” surface is absent in the Late Pleistocene early modern humans from East Asia such as Daoxian, Liujiang or Tianyuan Cave. In the present study, we analyzed the EDJ surface of 153 modern Chinese molars. Only 5.2% (8/153) showed a complex EDJ pattern and none reached the level of “dendrite-like” EDJ characteristic of the Asian H. erectus groups mentioned above (Table 1 , SI-Table7). In light of these comparisons, the complex EDJ surface morphologies of Dushan 1 molars should be considered a primitive character shared with Middle Pleistocene archaic Homo . Figure 5.þ Buccal EDJ views of right lower dentitions for Dushan 1 (A), Tianyuan Cave (only I2-M2 available) (B) and modern human. (C) e arrows indicate the pronounced buccal vertical groove, cingulum-like protostylid and complicated occlusal morphologies. (All the EDJ images are not scaled).

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8SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš Buccal basal swelling.þ e expression of a strong basal bulging at the buccal surface or premolars and molars is more frequently observed in earlier hominins (Australopithecus , early Homo and Middle Pleistocene Homo specimens from Asia) and is usually interpreted as a cingular derivative50, 56– 59. One of the most remarkable features of the upper and lower premolars and molars of Dushan 1 is the expression of a strong basal swelling of the buccal surface at the dentine level that in the case of the premolars is continuous with the marked marginal ridges (Figs 3 , 5 and 8 ) (see buccal vertical groove complex below) thereby forming a cingulum. e frequency of this feature in Middle Pleistocene Homo specimens from China is very high (50–90%) and decreases substantially in Late Pleistocene early modern humans (12.9–14.6%), with only more gracile expressions observed at the EDJ Figure 6.þ Roots and root canals of Dushan 1 premolars. I: right P3 and P4; II: right P3 and P4. DB: disto-buccal view; ML: mesio-lingual view (All the EDJ images are not scaled). Figure 7.þ Comparison of M1 occlusal morphologies at EDJ between Dushan 1 and comparative samples.

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9SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš level. is feature is very rare in contemporary modern humans (less than 10%) and can be regarded as a primi tive trait of Dushan 1 individual. Crown buccal vertical groove complex.þ Previous studies showed that Australopithecus and Homo habilis premo lars exhibit high frequencies of vertical grooves on the mesial and/or distal aspects of the buccal surfaces56, 59, 60. is feature was also identied in some Asian and European Early and Middle Pleistocene hominins with fre quencies ranging from 12% to 80%30, 31, 37, 50. In the present study, we found pronounced buccal grooves in the crown of all the upper and lower canines, and premolars of Dushan 1, and those were particularly pronounced at the EDJ surface. Similar grooves can also be seen in Dushan 1 lower molars although they constitute part of the protostylid. e premolars of some Chinese Middle Pleistocene Homo erectus exhibit a buccal vertical groove either at both the OES and EDJ (Hexian PA832 and HXUP, Zhoukoudian PA67, PA68, PA110) (ref.41, present study) or just at the EDJ (Yiyuan Sh.y. 003, Sh.y. 071)42. However, this feature is absent from nearly all the Late Pleistocene Chinese samples such as the Xujiayao, Liujiang and Tianyuan Cave sites. None of the recent modern human dentitions studied here revealed this feature. ere is a Neanderthal specimen (La Quina H18) that shows a well demarcated essential ridge and relatively pronounced vertical grooves at the EDJ, but these do not reach the degree of expression seen in Dushan 1, and they lack the pronounced angle between the basal part of the crown and the relatively depressed buccal slope of the buccal cusps. e involvement of canine, premolars, and molars is also unusual (Figs 3, 5 and 8). We name this composite feature as “crown buccal vertical groove complex”, and its expression in the upper and lower dentition of Dushan 1 can be considered a primitive trait. e result of the PCA based on eight non-metric traits for Dushan 1 and comparative samples show that Dushan 1 stays closer to the East Asian archaic Homo and early modern humans than to the recent modern humans with 79% variation represented by the rst two PCs (see SI-5 and SI-Fig.8 for detail). Figure 8.þ Buccal EDJ views of upper dentitions for Dushan 1 and comparative samples. (A) Dushan 1. (B) Liujiang. ( C) Neanderthal (La Quina H18). (E) Modern human.

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10SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš DiscussionIn the present study, we comprehensively analyzed the dental morphology of Dushan 1 individual, a terminal Pleistocene hominin from Southern China, by combining various metric and non-metric characters. rough comparisons with Middle Pleistocene Homo populations, Late Pleistocene early modern humans and recent modern humans as well as some reference to the variability of African early hominins, the morphological status of each dental character of Dushan 1 was determined. Although possible inter-character correlations and the exact taxonomic signicance of each trait are not fully understood, our comparisons are based on large samples of Pleistocene Homo and early and recent modern humans that can help us interpret the dental pattern of Dushan 1 more reliably. As shown in Table 1 , 15 out of 25 key traits in Dushan 1 are in common in either Eurasian Pleistocene archaic Homo (13 traits) or African early hominins (4 traits). Of those, only 10 traits are shared with modern humans. e mosaic combination of H. sapiens trait with an unusual number of primitive features and a few unique features makes Dushan 1 an “atypical H. sapiens” and raises questions about the evolutionary meaning of such a combination of dental characteristics in a terminal Pleistocene in Southern China. Among the “atypical” den tal traits of Dushan 1, we highlight: (1) an overall large dental size; (2) unusual dental size proportions that fall outside the range of variation of modern humans, including recent Chinese populations; (3) pronounced cingulum-like Carabelli’s trait and protostylid in molars; (4) the pronounced marginal ridges, and buccal vertical grooves on upper and lower premolars that merge with a basal bulge forming a cingulum-like structure; (5) the unusual high combination of ancestral features such as three-rooted P3, strongly and divergent bifurcated essential ridges of upper premolars, accessory marginal tubercles, strong development with conspicuous tip of the C5, expression of C6 and C7 (see SI-4);(5); the “dendrite-like” occlusal EDJ surface morphology in both upper and lower M1 and M2; (6) the rare “crown buccal vertical groove complex” from the canines to the third molars of both upper and lower dentitions. Due to the high variability that is characteristic of H. sapiens , some of these primitive or uncommon traits may not be taxonomically diagnostic per se, being occasionally present, albeit in very low percentages, in some recent modern humans27,28,30,33. Nevertheless, the combined expression of at least 13 characters listed in Table 1 (nos #3, #13, #14, #21, #22, #24, #27, #39, #49, #62, #63, #68, #69) for which Dushan 1 exhibits pronounced expression are more frequent and have more pronounced forms in archaic Homo and can easily dierentiate earlier hominins from modern human groups. In addition, the primitive dental traits of Dushan 1, such as the three-rooted P3 and the vertical grooves on the premolar crown buccal surface, are widely present in dierent Middle and Late Pleistocene hominins (Chinese H. erectus, European Middle Pleistocene hominins, Javanese H. erectus, and other later archaic Homo such as Xujiayao) or even more frequent in Australopithecus and early Homo specimens. Although the exact ontogenetic mechanism for multirooted P3s is not fully understood, and it seems to be controlled by either genetic or epigenetic factors, the available data indicate that multirooted mandibular premolars are early-evolved, highly conserved independent phenotypes that extends back to early primates. High occur rences of mandibular premolar multirooted variants are also observed in in our megadont hominin ancestors54. With all these in mind, the three-rooted P3s of Dushan 1 more possibly reect a primitive retention. In the later Pleistocene Homo species, the frequencies of the vertical grooves on the premolar crown buccal side decreased by a 30–40%. In Dushan individual, the grooves are present in upper and lower canines, premolars and molars and particularly pronounced at the EDJ surface, whereas none of our comparative samples of hominin fossil and recent modern humans with whole dentitions exhibits this composite character. Summarizing, our analyses reveal that Dushan 1 exhibits a special combination of primitive and unusual dental traits on the post-canine dentition together with some H. sapiens features. For the past ten years, several studies point to Southwest China and adjacent Southeast Asia as a key place to study the variability of Late Pleistocene hominins, including our own species11, 12, 19, 20, 24, 25. e analysis of Late Pleistocene samples like those from Maludong and Longlin have been interpreted as evidence of complex demographic patterns in Southwest China with several migrations and possible coexistence of H. sapiens with archaic hominins21– 23. In this context, nding a suite of primitive traits and a few unusual features in Dushan 1 provides additional information and fossil evidence for the variability and diversity of terminal Pleistocene human evolution in this region. ere are a number of alternative explanations for the mosaic dental pattern of Dushan 1. One alternative is that the primitive features expressed by Dushan are the result of introgressive hybridization with a late persisting archaic hominin. As mentioned above (Table 1), most of the primitive features identied in Dushan 1 are more oen found in Middle and early Late Pleistocene archaic Homo . Some special features, such as shelf-like Carabelli’s trait, are also found in the Late Pleistocene archaic hominins from Xujiayao. In addition, a series of mitochondrial and nuclear DNA analysis of a nger phalanx and two molars from the Denisovan remains revealed that the Denisovan genome has contributed 5% of the DNA to the genomes of present-day people in Oceania13, 14, 16. Recent studies show that the Late Pleistocene Xujiayao teeth share similarities to those of Denisovans including cingular remnant-like Carabelli’s cusps, strongly divergent roots and relatively large and complex crowns (refs8 , 45). All these studies suggest possible genetic contributions of late archaic hominins like Xujiayao and Denisovans to the ancestors of present-day populations across Asia and Oceania in addition to the Altai Mountains. e Denisovans may have lived in other parts of Asia61– 64. e relatively high level of Denisovan introgression in present-day Southeast Asian populations suggest that Denisovans had a wide geographical range, possibly including South China. In this context, the suite of primitive traits identied in Dushan 1 could be due to introgression from some late archaic hominins such as Denisovans into Dushan ancestors. However, the limited morphological data available for these late archaic hominins, including Denisovans, as well as the lack of genetic data from Dushan 1 prevents a proper testing of these hypotheses. A second alternative is that Dushan 1 represents one of the earliest H. sapiens populations inhabiting this region and, hence, the higher number of primitive traits retained by this group in comparison to later migrations.

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11SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš Recent studies of cranial, mandibular and dental morphologies of Middle and Late Pleistocene humans in Asia reveal high levels of variability. e combination of primitive and derived features does not follow a temporal gradient, and samples that are roughly contemporaneous may show a dierent combination of traits7 – 10, 41, 42. Based on these ndings, it was suggested that such a mosaic pattern reects an intermittent and/or discontinuous settlement of the continent. Fragmentation and isolation of the populations would have favored the retention of primitive features in certain groups and the development of new and/or more derived features in others65, 66. e present study shows that except for a group of primitive features, Dushan 1 upper and lower dentitions also exhibit some very rare or unique morphological features, including three-rooted P3 and a “crown buccal vertical groove complex”. Considering these, alternatively, we propose that Dushan 1 may represent a late survival of one of the earliest modern human groups to settle in an isolated region of Southern China and thus, that the unusual high retention of primitive traits in this population would be indicative of its early origin. In this case, Dushan 1 would be representing a particularity of the populations inhabiting this region.Materials and MethodsMaterials.þ Except the le M3, the whole upper and lower dentitions of Dushan 1 are available for study. Some teeth are attached to their corresponding maxillary or mandibular bone (le C1 to M2, right M1 to M3, right I2 to M1, and right M3) (Fig. 1, SI-Fig.3) whereas the rest are isolated (see SI-2 for details). Our study focuses on the comparison of Dushan 1 individual with fossil hominins and recent modern humans in the region. Considering its terminal Late Pleistocene age and its possible contribution to the understanding of the complex evolutionary scenario of East Asia in this period, Dushan 1 was compared with East Asian Middle Pleistocene archaic Homo , Late Pleistocene early modern humans and contemporary populations to assess its biological anities with them. Several recent modern human populations from archaeological collections in China are included, and some metric and non-metric data of global Homo sapiens samples from the literature are also used. e specimens of East Asian Middle Pleistocene archaic Homo comprised Chinese Homo erectus, and later archaic Homo members that were traditionally called “archaic Homo sapiens”. ese two groups have been pooled together to provide a broad frame of reference for the expression of primitive traits in continental East Asia and to dierentiate them from Homo sapiens sensu stricto (fossil and recent). To provide a broader view of Asian H. erectus , data from Javan Homo erectus are also included. Given their clear morphological dierences but also their contemporaneity with H. sapiens, the metric and non-metric data of Neanderthals were also included. Finally, when necessary, the expression of the relevant traits in Australopithecus , African Early and Middle Pleistocene Homo ( H. habilis and H. ergaster) and the European Early and Middle Pleistocene hominins was mentioned. Detailed information of the composition of the comparative samples is provided in SI-3 and SI-Table3. e EDJ and root morphology of Dushan 1 teeth were compared against a sample of Late Pleistocene and recent individuals preserving complete upper and lower dentitions (see SI-Table4 for details).Methods.þ Dushan 1 dentition was studied through the following analytical approaches: Traditional metric analyses.þ Mesiodistal (MD) and buccolingual (BL) dimensions of the crown were measured using a standard sliding caliper and recorded to the nearest 0.1þ t mm following34. Root length was measured from cervical line to the root tip on the buccal side. For the teeth embedded in the maxillary or mandibular bones, the measurement was made based on CT. With these metric data, crown sizes (MD and BL) of all the tooth types for both upper and lower dentitions were compared between Dushan 1 and comparative samples from East Asia. In order to compare dental size proportions, the crown size proportion between anterior teeth (I1 to C) and posterior teeth (P3 to M3), and molar size increase (%) were calculated18, 31. To further help interpret the crown size proportion of Dushan 1, we compared the BL widths of the upper and lower I2, P3, and M1 of Dushan 1 against those of several modern and fossil specimens including Neanderthals, and Late Pleistocene and recent modern human populations from China, the Levant, North Africa and the Canary Islands (SI-Table4). We ran three sets of analyses: one with all specimens that included the full set of maxillary dental measurements, one with all speci mens that included the full set of mandibular dental measurements, and one with all specimens that included the full set of both maxillary and mandibular dental measurements. In each case, we derived size-corrected metrics in the form of residual values from linear regressions of log-transformed BL width against the log-transformed geometric mean of all relevant dental measurements (maxillary, mandibular, or maxillary and mandibular). With each set, we ran a Principal Component Analysis (PCA) to visualize the shape of Dushan 1 in the context of shape variation in the entire sample. All analyses were conducted in R version 3.4.067. e results of this analysis are shown in SI-Figs 4–6. Non-metric trait comparison.þ Our study assessed both the external morphology of the original Dushan 1 teeth and the virtual reconstruction from micro-CT scanning of the EDJ and the root. Tooth wear stages were scored following the standards set by Molnar68. e terms employed for the non-metric description were taken from references27, 37, 49– 51, 60, 69. Some of the non-metric features were scored using the Arizona State University Dental Anthropology System (ASUDAS)27. Apart from the ASUDAS traits, in this study we have dened and recorded other traits we consider as relevant for the characterization of Dushan 1. To further explore the composite morphological information of the non-traits, we conduct PCA based on eight non-metric traits of I1 labial convexity, P3 bifurcated essential crest, P3 mesial and distal accessory ridge, P3 accessory marginal tubercle, M1 Carabelli’s trait, M1 protostylid, P3 crown buccal vertical groove and complicated occlusal morphology at EDJ for molars. e result of the analysis is provided in SI-5 and SI-Fig.8. Crown buccal groove of upper and lower postcanine dentitions.þ Previous studies showed that premolars of Australopithecus and early Homo frequently exhibit vertical grooves on the crown buccal side56, 59, 60. ese grooves

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12SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš delimit thickened marginal ridges that are commonly merged with a pronounced basal bulge or band-like prominence at the buccal surface. Our observation of Dushan 1 maxillary and mandibular dentitions indicates that the vertical grooves are present in all the post-canine teeth of this individual. erefore, this trait has been also recorded in our comparative samples (SI-Table5). Following the approach by Wood and Uytterschaut60 and Kaifu31, either if one or both of the buccal grooves are expressed we record the trait as present. Micro-CT scanning and reconstructions of the enamel dentine junction (EDJ) surface and roots.þ All Dushan 1 teeth and some specimens of comparative samples were scanned by high resolution microcomputed tomography (micro-CT) in order to virtually reconstruct the enamel-dentine junction (EDJ) and roots which are embed in the jaw bones. A 225 kVCT scanner was used to scan the specimens under the following settings: 140þ t kV, 120uA, 0.5 angular increment one step, 360 degrees of rotation, and the average of 4 frames. e isometric voxel size is 36.1þ t m. A total of 720 transmission images were reconstructed in a 2,0482,048 matrix of 1,195 slices. e RAW volume was imported into Mimics 19.0 to complete the virtual reconstruction. All the key traits observed, measured and compared in the present study are listed in SI-Table10. In addition, detailed morphological descriptions and comparisons for each individual tooth are also conducted (see SI-4).Data AvailabilityAll data generated or analyzed during this study are included in this published article (and its Supplementary Information Files).Referencesþt 1.þt Armitage, S. J. et al . e Southern oute “Out of Africa”: evidence for an early expansion of modern humans into Arabia. Science 331, 453–456 (2011).þt 2.þt Hershovitz, I. et al . Levantine cranium from Manot Cave (Israel) foreshadows the rst European modern humans. Nature 520, 216–219 (2015).þt 3.þt Bae, C. J., Petraglia, M. D., Doua, . Multidisciplinary Perspectives on the Human Evolution in Late Pleistocene Asia: Introduction to Supplement X. Current Anthropology (2017).þt 4.þt Liu, W. et al . Huanglong Cave: a Late Pleistocene human fossil site in Hubei Province. China. Quartern. Int. 211, 29–41 (2010).þt 5.þt Liu, W. et al . Human remains from Zhirendong, South China, and modern human emergence in East Asia. Proceedings of the Natural Academy of Science USA 107(45), 19201–19206 (2010).þt 6.þt Bae, C. et al . Modern human teeth from Late Pleistocene Luna Cave (Guangxi, China). Quatern. Int. 354, 169–183 (2014).þt 7.þt Liu, W. et al . e earliest unequivocally modern humans in Southern China. Nature 526, 696–699 (2015).þt 8.þt Xing, S. et al . Hominin teeth from the early Late Pleistocene site of Xujiayao, Northern China. American Journal of Physical Anthropology 156, 224–240 (2015).þt 9.þt Wu, X.-J. & Trinaus, E. e Xujiayao 14 mandibular ramus and Pleistocene Homo mandibular variation. Comptes endus Palevol. 13, 333–341 (2014).þt 10.þt Li, Z.-Y. et al . Late Pleistocene archaic human crania from Xuchang, China. Science 355, 969–972 (2017).þt 11.þt Brown, P. et al . A new small-bodied hominin from the Late Pleistocene of Flores, Indonesia. Nature 431, 1055–1061 (2004).þt 12.þt Morwood, M. J. et al . Further evidence for small-bodied hominins from the Late Pleistocene of Flores, Indonesia. Nature 437, 1012–1017 (2005).þt 13.þt rause, J. et al . e complete mitochondrial DNA genome of an unnown hominin from southern Siberia. Nature 464, 894–897 (2010).þt 14.þt Meyer, M. et al . A High-coverage genome sequence from an archaic Denisovan individual. Science 338, 222–226 (2012).þt 15.þt Fu, Q. et al . DNA analysis of an early modern human from Tianyuan Cave, China. Proceedings of the Natural Academy of Science USA 110, 2223–2227 (2013).þt 16.þt eich, D. et al . Genetic history of an archaic hominin group from Denisova Cave in Siberia. Nature 468, 1053–1060 (2010).þt 17.þt Pagani, L. et al . Tracing the peopling of the world through genomics. Nature 538, 538–542 (2016).þt 18.þt Shang, H. et al . An early modern human from Tianyuan Cave, Zhououdian, China. Proceedings of the Natural Academy of Science USA 104, 6573–6578 (2007).þt 19.þt ong, Q.-P. et al . Large-scale mtDNA screening reveals a surprising matrilineal complexity in East Asia and its implications to the peopling of the region. Mol. Biol. Evol. 28, 513–522 (2011).þt 20.þt Zhong, H. et al . Extended Y Chromosome Investigation Suggests Postglacial Migrations of Modern Humans into East Asia via the Northern oute. Biol. Evol. 28, 717–727 (2011).þt 21.þt Curnoe, D. et al . Human remains from the Pleistocene-Holocene transition of Southwest China suggest a complex evolutionary history for East Asians. PLoS ONE 7, e31918 (2012).þt 22.þt Curnoe, D. et al . Possible signatures of hominin hybridization from the Early Holocene of Southwest China. Scientic eports 5 , 12408 (2015).þt 23.þt Curnoe, D. et al . A Hominin femur with archaic anities from the Late Pleistocene of Southwest China. PLoS ONE 10, e01433 (2015).þt 24.þt Demeter, F. et al . Anatomically modern human in Southeast Asia (Laos) by 46 a. Proceedings of the Natural Academy of Science USA 109, 14375–14380 (2012).þt 25.þt Demeter, F. et al . Early modern humans and morphological variation in Southeast Asia: fossil evidence from Tam Pa Ling, Laos. PLoS One e0121193 (2015).þt 26.þt Trinaus, E. Modern human versus Neandertal evolutionary distinctiveness. Current Anthropology 47, 597–620 (2006).þt 27.þt Scott, G. ., Turner, C. G. The anthropology of modern human teeth: dental morphology and its variation in recent human populations. Vol. 20 (Cambridge University Press, 1997).þt 28.þt Bailey, S. E., Hublin, J. J. What does it mean to be dentally “modern”? In: Scott, G. & Irish, J. D, editors. Anthropological Perspectives on Tooth Morphology: Genetics, Evolution, Variation. Cambridge: Cambridge Univ. Press, 222–249 (2013).þt 29.þt Burnett, S. E., Hawey, D. E. & Turner, C. G. Brief Communication: Population Variation in Human Maxillary Premolar Accessory idges (MxPA). American Journal of Physical Anthropology 141, 319–324 (2010).þt 30.þt Martinón-Torres, M. et al . Morphological description and comparison of the dental remains from Atapuerca-Sima de los Huesos site (Spain). Journal of Human Evolution 62, 7–58 (2012).þt 31.þt aifu, Y. et al . Unique dental morphology of Homo oresiensis and its evolutionary implications. PLoS ONE 10(11), e0141614 (2015).þt 32.þt Abbott, S. A. A comparative study of tooth root morphology in the great apes, modern man and early hominids. Ph.D. Dissertation. University of London (1984).þt 33.þt Scott, ., Irish, J. Human Tooth Crown and oot Morphology. The Arizona State University Dental Anthropology System. Cambridge University Press (2017).þt 34.þt Wolpo, M. Metric Trends in Hominid Dental Evolution. Cleveland: Case Western eserve University, (1971).

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13SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš þt 35.þt Bermúdez de Castro, J. M. & Nicolas, M. E. Posterior dental size reduction in hominids: the Atapuerca evidence. American Journal of Physical Anthropology 96, 335–356 (1995).þt 36.þt Martinón-Torres, M. et al . Dental evidence on the hominin dispersals during the Pleistocene. Proceedings of the Natural Academy of Science USA 104, 13279–13282 (2007).þt 37.þt Martinon-Torres, M. et al . Dental remains from Dmanisi (epublic of Georgia): Morphological analysis and comparative study. Journal of Human Evolution 56, 76–85 (2008).þt 38.þt Ortiz, A. et al . Carabelli’s trait revisited: An examination of mesiolingual features at the enamel dentine junction and enamel surface of Pan and Homo sapiens upper molars. Journal of Human Evolution 63, 586–596 (2012).þt 39.þt Van eenen, J. F., eid, C. eCarabelli trait in early South African hominids: a morphological study. In: Moggi-Cecchi, J. (Ed.), Aspects of Dental Biology: Paleontology, Anthropology and Evolution. International Institute for the Study of Man, Florence, 291–298 (1995).þt 40.þt Wood, B. A. & Engleman, C. A. Analysis of the dental morphology of Plio-Pleistocene hominids, V. Maxillary postcanine tooth morphology. J. Anat. 161, 1–35 (1988).þt 41.þt Xing, S. et al . Middle Pleistocene hominin teeth from Longtan Cave, Hexian, China. PLoS ONE 9, e114265 (2014).þt 42.þt Xing, S. et al . Hominin teeth from the Middle Pleistocene site of Yiyuan, Eastern China. Journal of Human Evolution 95, 33–54 (2016).þt 43.þt Zanolli, C. Additional evidence for morpho-dimensional tooth crown variation in a new Indonesian H. erectus sample from the Sangiran Dome (Central Java). PLoS One 8, e67233 (2013).þt 44.þt Zonolli, C. Brief Communication: Molar crown inner structural organization in Javanese Homo erectus. American Journal of Physical Anthropology 156, 148–157 (2015).þt 45.þt Zubova, A. V., Chiisheva, T. A. & Shunov, M. V. e Morphology of Permanent Molars from the Paleolithic Layers of Denisova Cave. Archaeology, Ethnology & Anthropology of Eurasia 45/1, 121–134 (2017).þt 46.þt Wood, B. A. & Abbott, S. A. Analysis of the dental morphology of Plio-Pleistocene hominids. I. Mandibular molars: crown area measurements and morphological traits. J. Anat. 136, 197–219 (1983).þt 47.þt Hluso, L. Protostylid variation in Australopithecus . Journal of Human Evolution 46, 579–594 (2002).þt 48.þt Sinner, M. M., Wood, B. A. & Hublin, J. J. Protostylid expression at the enamel-dentine junction and enamel surface of mandibular molars of Paranthropus robustus and Australopithecus africanus . Journal of Human Evolution 56, 76–85 (2009).þt 49.þt Wood, B. A., Abbott, S. A. & Uytterschaut, H. Analysis of the dental morphology of Plio-Pleistocene hominids. IV. Mandibular postcanine root morphology. J Anat. 156, 107–139 (1988).þt 50.þt Weidenreich, F. e dentition of Sinanthropus peinensis: a comparative odontography of the hominids. Vol. 1 1–180 (1937).þt 51.þt Bermúdez de Castro, J. M., osas, A. & Nicolás, M. A. Dental remains from Atapuerca-TD6 (Gran Dolina site, Burgos, Spain). Journal of Human Evolution 37, 523–566 (1999).þt 52.þt Prado-Simon, L. et al . A morphological study of the tooth roots of the Sima del Elefante mandible (Atapuerca, Spain): a new classication of the teeth — biological and methodological considerations. Anthropological Science 120, 61–72 (2012).þt 53.þt Selmer-Olsen, . An odontometrical study on the Norwegian Lapps. Det Norse Vidensaps Aademi, Oslo (1949).þt 54.þt Shields, E. D. Mandibular premolar and second molar root morphological variation in modern humans: what root number can tell us about tooth morphogenesis. American Journal of Physical Anthropology 128, 299–311 (2005).þt 55.þt Moore, N. C. et al . Premolar root and canal variation in South African Plio-Pleistocene specimens attributed to Australopithecus africanus and Paranthropus robustus . Journal of Human Evolution 93, 46–62 (2016).þt 56.þt obinson, J. T. e Dentition of the Australopithecinae. Pretoria: Transvaal Museum (1956).þt 57.þt Johanson, D. C., White, T. D. & Coppens, Y. Dental remains from the Hadar Formation, Ethiopia: 1974–1977 collections. American Journal of Physical Anthropology 57, 545–603 (1982).þt 58.þt Ward, C. V., Leaey, M. G. & Waler, A. Morphology of Australopithecus anamensis from anapoi and Allia Bay, enya. Journal of Human Evolution 41, 255–368 (2001).þt 59.þt Suwa, G. A comparative analysis of hominid dental remains from the Sungura and Usno Formations, Omo Valley, Ethiopia. PhD dissertation, University of California at Bereley (1990).þt 60.þt Wood, B. & Uytterschaut, H. Analysis of the dental morphology of Plio-Pleistocene hominids. III. Mandibular premolar crowns. J Anat. 154, 121–156 (1988).þt 61.þt eich, D. et al . Denisova admixture and the rst modern human dispersals into Southeast Asia and Oceania. Am J Hum Genet. 89, 516–528 (2011).þt 62.þt Prüfer, . et al . e complete genome sequence of a Neanderthal from the Altai Mountains. Nature 505, 43–49 (2014).þt 63.þt Sawyer, S. et al . Nuclear and mitochondrial DNA sequences from two Denisovan individuals. Proceedings of the Natural Academy of Science USA 112, 15696–15700 (2015).þt 64.þt ogersa, A., Bohlender, . & Hu, C. Early history of Neanderthals and Denisovans. Proceedings of the Natural Academy of Science USA 112, 9859–9863 (2017).þt 65.þt Martinón-Torres, M. et al . Homo sapiens in the Late Pleistocene: What Asia has to tell? Current Anthropology 58(S17), 434–448 (2017).þt 66.þt Martinón-Torres, M. et al . A “source and sin” model for East Asia? Preliminary approach through the dental evidence. Comptes endus Palevol. 17, 33–43 (2018).þt 67.þt Core Team. A language and environment for statistical computing. Foundation for Statistical Computing, Vienna, Austria , https://www.-project.org/ (2017).þt 68.þt Molnar, S. Human tooth wear, tooth function and cultural variability. American Journal of Physical Anthropology 34, 175–189 (1971).þt 69.þt Bermúdez de Castro, J. M. Dental remains from Atapuerca/Ibeas (Spain) II. Morphology. Journal of Human Evolution 17, 279–304 (1988).…‘™Ž‡†‰‡‡–•e authors would thank all the colleagues and institutions that have helped us in this research. We would express our gratitude to Drs. Yousuke Kaifu, Richard Scott, Joel Irish and Scott Burnett for providing references and helpful discussion. Thanks to Dr. Lei Pan for her help with the observation and references of roots of Australopithecus and African early Homo . is work has been supported by grants from Chinese Academy of Sciences (XDB26000000, 132311KYSB20160004), National Natural Science Foundation of China (41572023, 41630102, 41672020,41872030), BaGui Scholars Project of the Guangxi Zhuang Autonomous Region, Ministerio de Economía y Competitividad/FEDER (CGL2015-65387-C3-3-P), Biritish Academy (International Partnership and Mobility Scheme PM160019) and the Leakey Foundation through the personal support of Dub Crook (2014, 2015, 2016, 2018) and Gordon Getty (2012) to one of the authors (MMT).

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14SCIENTIFIC REPOþrRTS | (2019) 9:2347 Š––’•ã†‘‹ä‘”‰wväwvy~•zw{~ævwæy~~w~æš Author ContributionsW.W. designed Dushan Cave research project. Wei Liao, S.X., D.W.L., M.M.T., X.W., J.M.B.C., W.L. and W.W. contributed to the data collection. W.W. and D.W.L. carried out the excavation. Wei Liao analyzed stratigraphic context and conducted chronological samples. S.X., M.M.T., C.S., X.W. and W.L. analyzed dental data. Wei Liao, M.M.T., J.M.B.C., C.S. and W.L. wrote the paper. All authors reviewed the manuscript.Additional InformationSupplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-38818-x. Competing Interests : e authors declare no competing interests. Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional aliations. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. e images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © e Author(s) 2019


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