Identification of Diverse Alphacoronaviruses and Genomic Characterization of a Novel Severe Acute Respiratory Syndrome-Like Coronavirus from Bats in China

Citation

Material Information

Title:
Identification of Diverse Alphacoronaviruses and Genomic Characterization of a Novel Severe Acute Respiratory Syndrome-Like Coronavirus from Bats in China
Series Title:
Journal of Virology
Creator:
He, Biao
Zhang, Yuzhen
Xu, Lin
Yang, Weihong
Yang, Fanli
Feng, Yung
Xia, Lele
Zhou, Jihua
Zhen, Weibin
Feng, Ye
Guo, Huancheng
Zhang, Hailin
Tu, Changchun
Publisher:
American Society for Microbiology
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
Identification Of Coronaviruses ( local )
Diverse Coronaviruses ( local )
Alphacoronaviruses ( local )
Genomic Characterization ( local )
Novel Coronavirus ( local )
Sars ( local )
Bats ( local )
Bats In China ( local )
China ( local )
Genre:
serial ( sobekcm )

Notes

Abstract:
Although many severe acute respiratory syndrome-like coronaviruses (SARS-like CoVs) have been identified in bats in China, Europe, and Africa, most have a genetic organization significantly distinct from human/civet SARS CoVs in the receptor-binding domain (RBD), which mediates receptor binding and determines the host spectrum, resulting in their failure to cause human infections and making them unlikely progenitors of human/civet SARS CoVs. Here, a viral metagenomic analysis of 268 bat rectal swabs collected from four counties in Yunnan Province has identified hundreds of sequences relating to alpha- and betacoronaviruses. Phylogenetic analysis based on a conserved region of the RNA-dependent RNA polymerase gene revealed that alphacoronaviruses had diversities with some obvious differences from those reported previously. Full genomic analysis of a new SARS-like CoV from Baoshan (LYRa11) showed that it was 29,805 nucleotides (nt) in length with 13 open reading frames (ORFs), sharing 91% nucleotide identity with human/civet SARS CoVs and the most recently reported SARS-like CoV Rs3367, while sharing 89% with other bat SARS-like CoVs. Notably, it showed the highest sequence identity with the S gene of SARS CoVs and Rs3367, especially in the RBD region. Antigenic analysis showed that the S1 domain of LYRa11 could be efficiently recognized by SARS-convalescent human serum, indicating that LYRa11 is a novel virus antigenically close to SARS CoV. Recombination analyses indicate that LYRa11 is likely a recombinant descended from parental lineages that had evolved into a number of bat SARS-like CoVs.
Original Version:
Journal of Virology, Vol. 88, no. 12 (2014-05-21).

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This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.

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