Longitudinal surveillance of SARS-like coronaviruses in bats by quantitative real-time PCR

Longitudinal surveillance of SARS-like coronaviruses in bats by quantitative real-time PCR

Material Information

Longitudinal surveillance of SARS-like coronaviruses in bats by quantitative real-time PCR
Series Title:
Virologica sinica
Wang, Mei-Niang
Zhang, Wei
Gao, Yu-Tao
Hu, Ben
Ge, Xing-Yi
Yang, Xing-Lou
Zhang, Yun-Zhi
Shi, Zheng-Li
Springer Nature
Publication Date:
Physical Description:
1 online resource


Subjects / Keywords:
Coronaviruses ( lcsh )
SARS (Disease) ( lcsh )
Bats -- Viruses ( lcsh )
Polymerase chain reaction ( lcsh )
serial ( sobekcm )
Asia -- China -- Yunnan


We conducted a longitudinal surveillance study of bat SL-CoVs using quantitative real-time PCR (qRT-PCR) targeting the nucleocapsid (N) and RNA-dependent RNA polymerase (RdRp) genes in one bat population in Yunnan, China. A total of 431 bat fecal samples were collected during 2011–2014.Fifty-seven of 431 bat fecal samples were positive for SL-CoVs by RT-PCR screening. The detection rate varied significantly among sampling dates (ANOVA,F=28.42, P= 0.03), from 3.1% to 48.7%. The N and RdRp assays revealed dynamic changes in SL-CoV concentrations in the longitudinal surveillance. Fecal pellets with high virus concentrations were typically collected in either September 2012 or July 2013. Additionally, the average virus concentrations in July, September, and October were over 5-fold higher than those observed in April and May. These results indicated that SL-CoV amplification was more efficient from the late summer to autumn. (Letter to the editor)
Original Version:
Volume 31, Issue 1

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Source Institution:
University of South Florida
Holding Location:
University of South Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
K26-05435 ( USFLDC DOI )
k26.5435 ( USFLDC Handle )

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University of South Florida
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