Expression of biological activity of draculin, the anticoagulant factor from vampire bat saliva, is strictly dependent on the appropriate glycosylation of the native molecule.

Citation

Material Information

Title:
Expression of biological activity of draculin, the anticoagulant factor from vampire bat saliva, is strictly dependent on the appropriate glycosylation of the native molecule.
Series Title:
Biochimica et Biophysica Acta (BBA) - General Subjects
Creator:
Fernandez, Ana Z.
Tablante, Alfonso
Bartoli, Fulvia
Beguin, Suzette
Hemker, H.C.
Apitz-Castro, Rafael
Publisher:
Elsevier
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
Draculin ( local )
Glycosylation ( local )
Natural Glycoform ( local )
Anticoagulant ( local )
Genre:
serial ( sobekcm )

Notes

Abstract:
Draculin, a glycoprotein isolated from vampire bat (Desmodus rotundus) saliva, is a natural anticoagulant which inhibits activated coagulation factors IX (IXa) and X (Xa). The observation that under captivity conditions, the anticoagulant activity present in vampire bat saliva is dependent upon the salivation protocol, led us to investigate the possible relationship between the expression of biological activity of native draculin and the post-translational glycosylation of the protein backbone. Daily salivation of vampire bats yields a saliva that progressively decreases in anticoagulant activity, without any significant change in overall protein content, or in the amount of protein specifically recognized by a polyclonal anti-draculin antibody. Anticoagulant activity of the saliva is restored after a 4-day period of rest. Besides the marked difference in anticoagulant activity, purified native draculin, obtained from high- and low-activity saliva, shows significant differences in: (a) composition of the carbohydrate moiety, and (b) Glycosylation pattern. Furthermore, controlled chemical deglycosylation of native draculin, under conditions that do not affect the polypeptide backbone, progressively leads to complete loss of the biological activity. Our present results implicate that correct glycosylation of draculin is a seminal event for the expression of the biological activity of this glycoprotein.
Original Version:
Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1425, no. 2 (1998-10-23).

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