Title Expression of biological activity of draculin, the anticoagulant factor from vampire bat saliva, is strictly dependent on the appropriate glycosylation of the native molecule.
Abstract
Draculin, a glycoprotein isolated from vampire bat (Desmodus rotundus) saliva, is a natural anticoagulant which inhib... Publication Date
1998-10-23
Holding Location
University of South Florida
Language
English
Creator
Fernandez, Ana Z. Tablante, Alfonso Bartoli, Fulvia Beguin, Suzette Hemker, H.C. Apitz-Castro, Rafael Publisher
Elsevier Source Institution
University of South Florida Library
Subjects / Keywords
Draculin Glycosylation Natural Glycoform Anticoagulant Series Title
Biochimica et Biophysica Acta (BBA) - General Subjects Aggregation
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serial
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Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1425, no. 2 (1998-10-23).
This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.
Expression of biological activity of draculin, the anticoagulant factor from vampire bat saliva, is strictly dependent on the appropriate glycosylation of the native molecule.
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https://digital.lib.usf.edu/SFS0069814/00001
Material Information
Title:
Expression of biological activity of draculin, the anticoagulant factor from vampire bat saliva, is strictly dependent on the appropriate glycosylation of the native molecule. Series Title:
Biochimica et Biophysica Acta (BBA) - General Subjects
Creator:
Fernandez, Ana Z.
Tablante, Alfonso
Bartoli, Fulvia
Beguin, Suzette
Hemker, H.C.
Apitz-Castro, Rafael
Publisher:
Elsevier Publication Date:
1998-10-23 Language:
English
Subjects
Subjects / Keywords:
Draculin ( local )
Genre:
serial ( sobekcm )
Notes
Abstract:
Draculin, a glycoprotein isolated from vampire bat (Desmodus rotundus) saliva, is a natural anticoagulant which inhibits activated coagulation factors IX (IXa) and X (Xa). The observation that under captivity conditions, the anticoagulant activity present in vampire bat saliva is dependent upon the salivation protocol, led us to investigate the possible relationship between the expression of biological activity of native draculin and the post-translational glycosylation of the protein backbone. Daily salivation of vampire bats yields a saliva that progressively decreases in anticoagulant activity, without any significant change in overall protein content, or in the amount of protein specifically recognized by a polyclonal anti-draculin antibody. Anticoagulant activity of the saliva is restored after a 4-day period of rest. Besides the marked difference in anticoagulant activity, purified native draculin, obtained from high- and low-activity saliva, shows significant differences in: (a) composition of the carbohydrate moiety, and (b) Glycosylation pattern. Furthermore, controlled chemical deglycosylation of native draculin, under conditions that do not affect the polypeptide backbone, progressively leads to complete loss of the biological activity. Our present results implicate that correct glycosylation of draculin is a seminal event for the expression of the biological activity of this glycoprotein. Original Version:
Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1425, no. 2 (1998-10-23).
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University of South Florida Library Holding Location:
University of South Florida
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This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the bounds of Fair Use or other exemptions to copyright law must be obtained from the copyright holder.
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